SC5AB_MOUSE
ID SC5AB_MOUSE Reviewed; 673 AA.
AC Q8K0E3; Q6EZ51;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Sodium/myo-inositol cotransporter 2;
DE Short=Na(+)/myo-inositol cotransporter 2;
DE AltName: Full=Sodium-dependent glucose cotransporter;
DE AltName: Full=Sodium/glucose cotransporter KST1;
DE AltName: Full=Sodium/myo-inositol transporter 2;
DE Short=SMIT2;
DE AltName: Full=Solute carrier family 5 member 11;
GN Name=Slc5a11 {ECO:0000312|EMBL:AAH31742.1};
GN Synonyms=Kst1 {ECO:0000303|Ref.1}, Smit2 {ECO:0000250|UniProtKB:Q28728};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAQ08402.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAQ08402.1};
RA Mount D.B., Mercado A.;
RT "Sequence of the murine KST1 sodium-solute cotransporter, alternatively
RT spliced isoform.";
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAH31742.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N {ECO:0000312|EMBL:AAH31742.1};
RC TISSUE=Kidney {ECO:0000312|EMBL:AAH31742.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in the sodium-dependent cotransport of myo-inositol
CC (MI) with a Na(+):MI stoichiometry of 2:1. Exclusively responsible for
CC apical MI transport and absorption in intestine. Can also transport D-
CC chiro-inositol (DCI) but not L-fructose. Exhibits stereospecific
CC cotransport of both D-glucose and D-xylose. May induce apoptosis
CC through the TNF-alpha, PDCD1 pathway. May play a role in the regulation
CC of MI concentration in serum, involving reabsorption in at least the
CC proximal tubule of the kidney (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q28728}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:Q28728}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:15489334};
CC IsoId=Q8K0E3-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|Ref.1};
CC IsoId=Q8K0E3-2; Sequence=VSP_052788;
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ08402.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF518726; AAQ08402.1; ALT_FRAME; mRNA.
DR EMBL; BC031742; AAH31742.1; -; mRNA.
DR CCDS; CCDS21819.1; -. [Q8K0E3-1]
DR RefSeq; NP_666310.1; NM_146198.2. [Q8K0E3-1]
DR RefSeq; XP_006507775.1; XM_006507712.3. [Q8K0E3-1]
DR RefSeq; XP_011240068.1; XM_011241766.2. [Q8K0E3-1]
DR RefSeq; XP_011240069.1; XM_011241767.2. [Q8K0E3-1]
DR RefSeq; XP_011240070.1; XM_011241768.2. [Q8K0E3-1]
DR AlphaFoldDB; Q8K0E3; -.
DR SMR; Q8K0E3; -.
DR STRING; 10090.ENSMUSP00000033035; -.
DR PhosphoSitePlus; Q8K0E3; -.
DR SwissPalm; Q8K0E3; -.
DR jPOST; Q8K0E3; -.
DR PaxDb; Q8K0E3; -.
DR PRIDE; Q8K0E3; -.
DR ProteomicsDB; 255470; -. [Q8K0E3-1]
DR ProteomicsDB; 255471; -. [Q8K0E3-2]
DR Antibodypedia; 12746; 140 antibodies from 26 providers.
DR DNASU; 233836; -.
DR Ensembl; ENSMUST00000033035; ENSMUSP00000033035; ENSMUSG00000030769. [Q8K0E3-1]
DR Ensembl; ENSMUST00000167299; ENSMUSP00000127977; ENSMUSG00000030769. [Q8K0E3-1]
DR GeneID; 233836; -.
DR KEGG; mmu:233836; -.
DR UCSC; uc009jpg.1; mouse. [Q8K0E3-1]
DR CTD; 115584; -.
DR MGI; MGI:1919316; Slc5a11.
DR VEuPathDB; HostDB:ENSMUSG00000030769; -.
DR eggNOG; KOG2349; Eukaryota.
DR GeneTree; ENSGT00940000157690; -.
DR HOGENOM; CLU_018808_9_2_1; -.
DR InParanoid; Q8K0E3; -.
DR OMA; GWWGMRR; -.
DR OrthoDB; 243316at2759; -.
DR PhylomeDB; Q8K0E3; -.
DR TreeFam; TF352855; -.
DR Reactome; R-MMU-429593; Inositol transporters.
DR BioGRID-ORCS; 233836; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Slc5a11; mouse.
DR PRO; PR:Q8K0E3; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8K0E3; protein.
DR Bgee; ENSMUSG00000030769; Expressed in small intestine Peyer's patch and 51 other tissues.
DR ExpressionAtlas; Q8K0E3; baseline and differential.
DR Genevisible; Q8K0E3; MM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005412; F:glucose:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0032409; P:regulation of transporter activity; IDA:MGI.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..673
FT /note="Sodium/myo-inositol cotransporter 2"
FT /id="PRO_0000331569"
FT TOPO_DOM 1..27
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..102
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..180
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..272
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..308
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..375
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..418
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 440..446
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 468..479
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 501..521
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 543..652
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 653..673
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 403..626
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_052788"
FT CONFLICT 22
FT /note="K -> EG (in Ref. 1; AAQ08402)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="N -> S (in Ref. 1; AAQ08402)"
FT /evidence="ECO:0000305"
FT CONFLICT 359
FT /note="I -> V (in Ref. 1; AAQ08402)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 673 AA; 73798 MW; C00DA80F9F02140D CRC64;
MESATISPQP PQSDSLEAFP QKSMEPADIA VLVLYFLFVL AVGLWSTVRT KRDTVKGYFL
AGGDMVWWPV GASLFASNVG SGHFIGLAGS GAAVGISVAA YELNGLFSVL MLAWVFLPIY
IAGQVTTMPE YLRRRFGGNR ISITLAVLYL FIYIFTKISV DMYAGAIFIQ QSLHLDLYLA
IVGLLAITAL YTVAGGLAAV IYTDALQTVI MLIGAFILMG YSFAAVGGME GLKDQYFLAL
ASNRSENSSC GLPREDAFHI FRDPLTSDLP WPGILFGMSI PSLWYWCTDQ VIVQRSLAAK
NLSHAKGGSL MAAYLKVLPL FLMVFPGMVS RVLFPDQVAC AHPDICQRVC SNPSGCSDIA
YPKLVLELLP TGLRGLMMAV MVAALMSSLT SIFNSASTIF TMDLWNHIRP RASERELMIV
GRIFVFALVL VSILWIPIVQ ASQGGQLFIY IQSISSYLQP PVAMVFIMGC FWKRTNEKGA
FSGLILGLLL GLVRLILDFV YAQPRCDQPD DRPAVVKDVH YLYFSMILSF TTLITVVTVS
WFTETPSKEM VSRLTWFTRH EPVAQKDSAP PETPLSLTLS QNGTTEAPGT SIQLETVQES
TTKACGDGVS PRHSKVVRAI LWLCGMEKNK EEPPSKAEPV IVSLEENPLV KTLLDVNCIV
CISCAIFLWG YFA
