SC5AC_BOVIN
ID SC5AC_BOVIN Reviewed; 617 AA.
AC A7MBD8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Sodium-coupled monocarboxylate transporter 2;
DE AltName: Full=Electroneutral sodium monocarboxylate cotransporter;
DE AltName: Full=Low-affinity sodium-lactate cotransporter;
DE AltName: Full=Solute carrier family 5 member 12;
GN Name=SLC5A12; Synonyms=SMCT2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Kidney;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as an electroneutral and low-affinity sodium (Na(+))-
CC dependent sodium-coupled solute transporter. Catalyzes the transport
CC across the plasma membrane of many monocarboxylates such as lactate,
CC pyruvate, nicotinate, propionate, butyrate and beta-D-hydroxybutyrate.
CC May be responsible for the first step of reabsorption of
CC monocarboxylates from the lumen of the proximal tubule of the kidney
CC and the small intestine. May play also a role in monocarboxylates
CC transport in the retina. Mediates electroneutral uptake of lactate,
CC with a stoichiometry of 2 Na(+) for each lactate (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Detected at the brush border
CC membrane of the kidney. Colocalizes with viementin in Mueller cells (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; BC151502; AAI51503.1; -; mRNA.
DR RefSeq; NP_001094529.1; NM_001101059.2.
DR AlphaFoldDB; A7MBD8; -.
DR SMR; A7MBD8; -.
DR STRING; 9913.ENSBTAP00000029140; -.
DR PaxDb; A7MBD8; -.
DR Ensembl; ENSBTAT00000029140; ENSBTAP00000029140; ENSBTAG00000021858.
DR GeneID; 507621; -.
DR KEGG; bta:507621; -.
DR CTD; 159963; -.
DR VEuPathDB; HostDB:ENSBTAG00000021858; -.
DR VGNC; VGNC:34905; SLC5A12.
DR eggNOG; KOG2349; Eukaryota.
DR GeneTree; ENSGT00940000159545; -.
DR HOGENOM; CLU_018808_11_1_1; -.
DR InParanoid; A7MBD8; -.
DR OMA; GFFSHTM; -.
DR OrthoDB; 1180002at2759; -.
DR TreeFam; TF316728; -.
DR Reactome; R-BTA-427601; Multifunctional anion exchangers.
DR Proteomes; UP000009136; Chromosome 15.
DR Bgee; ENSBTAG00000021858; Expressed in metanephros cortex and 32 other tissues.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0015129; F:lactate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IBA:GO_Central.
DR CDD; cd11520; SLC5sbd_SMCT2; 1.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR042700; SMCT2_SLC5sbd.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..617
FT /note="Sodium-coupled monocarboxylate transporter 2"
FT /id="PRO_0000337679"
FT TOPO_DOM 1..5
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..180
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..235
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..334
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..385
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..411
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..507
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 529..617
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 219
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 480
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 617 AA; 67410 MW; 8FC60B8C1B3F795E CRC64;
MEVKNFAVWD YVVFAALFII SSGIGVFYAI KERKKATSRE FLVGGRQMSF GPVALSLTAS
FMSAVTVLGT PADVYRFGAS FVLFFITYGL VIILTSELFL PVFYRSGITS TYEYLQLRFN
KPVRYAATVI YIVQTILYTG VVVYAPALAL NQVTGFDLWG SVFATGIVCT FYCTLGGLKA
VVWTDAFQMV VMIVGFLTVL IQGSTYAGGL HNVLEQAENG SRLNIFDFDI DPLRRHTFWT
ISVGGTFTWL GIYGVNQSTI QRCISCKTEK HAKLALYFNL LGLWIILLCA VFSGLTMYAH
FKDCDPWTSG IISAPDQLMP YFVMELFSTM PGLPGLFVAC AFSGTLSTVA ASINALATVT
FEDFVKSCFP RLSDKLSTWI SKGLCLLFGV ICTSTAVAAS LMGGVIQAAL SIHGMCGGPM
LGLFSLGILF PFVNWKGALA GLLTGILLSF WVAIGAFIYP APASKTWPLP LSTDQCGLSN
VTESVPPVLS SRPAIAETWY ALSYLHYSTV GCLGCIAAGV IISFLTGLQK GKDIPPLLIR
PVCNLFCFWS KKYKTLCWCG VQHDSGTEQE NLENDSTWKQ GAESVLQNGL KQESLVPGYD
PKDKSYDNMA LEKITHF
