SC5AC_DANRE
ID SC5AC_DANRE Reviewed; 623 AA.
AC Q7T384;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Sodium-coupled monocarboxylate transporter 2;
DE AltName: Full=Electroneutral sodium monocarboxylate cotransporter;
DE Short=zSMCTn;
DE AltName: Full=Low-affinity sodium-lactate cotransporter;
DE AltName: Full=Solute carrier family 5 member 12;
DE Short=zSLC5A12;
GN Name=slc5a12; Synonyms=smctn;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=17255103; DOI=10.1074/jbc.m609313200;
RA Plata C., Sussman C.R., Sindic A., Liang J.O., Mount D.B., Josephs Z.M.,
RA Chang M.-H., Romero M.F.;
RT "Zebrafish Slc5a12 encodes an electroneutral sodium monocarboxylate
RT transporter (SMCTn). A comparison with the electrogenic SMCT
RT (SMCTe/Slc5a8).";
RL J. Biol. Chem. 282:11996-12009(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as an electroneutral and low-affinity sodium (Na(+))-
CC dependent sodium-coupled solute transporter. Catalyzes the transport
CC across the plasma membrane of many monocarboxylates such as lactate,
CC pyruvate, nicotinate, propionate, butyrate and beta-D-hydroxybutyrate.
CC {ECO:0000269|PubMed:17255103}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.81 mM for lactate {ECO:0000269|PubMed:17255103};
CC KM=2.03 mM for pyruvate {ECO:0000269|PubMed:17255103};
CC KM=23.68 mM for nicotinate {ECO:0000269|PubMed:17255103};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed in brain and eye 24, 72 and 120 hours
CC post-fertilization (hpf). At 72 hpf expressed in swim bladder and in
CC pronephric tube. At 120 hpf expressed in brain, trabecular bar, eyes,
CC otic capsule, stomach, gall bladder, gut and pancreas.
CC {ECO:0000269|PubMed:17255103}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY727860; AAW55812.1; -; mRNA.
DR EMBL; BC053215; AAH53215.1; -; mRNA.
DR RefSeq; NP_956662.1; NM_200368.1.
DR AlphaFoldDB; Q7T384; -.
DR SMR; Q7T384; -.
DR STRING; 7955.ENSDARP00000128187; -.
DR TCDB; 2.A.21.5.4; the solute:sodium symporter (sss) family.
DR PaxDb; Q7T384; -.
DR GeneID; 393339; -.
DR KEGG; dre:393339; -.
DR CTD; 159963; -.
DR ZFIN; ZDB-GENE-040426-1343; slc5a12.
DR eggNOG; KOG2349; Eukaryota.
DR InParanoid; Q7T384; -.
DR OrthoDB; 1180002at2759; -.
DR PhylomeDB; Q7T384; -.
DR Reactome; R-DRE-427601; Multifunctional anion exchangers.
DR PRO; PR:Q7T384; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015355; F:secondary active monocarboxylate transmembrane transporter activity; IDA:ZFIN.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IBA:GO_Central.
DR CDD; cd11520; SLC5sbd_SMCT2; 1.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR042700; SMCT2_SLC5sbd.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..623
FT /note="Sodium-coupled monocarboxylate transporter 2"
FT /id="PRO_0000337682"
FT TOPO_DOM 1..10
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..50
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..158
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..181
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..236
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..322
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..386
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 408..412
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 434..438
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 532..623
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 465
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 478
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 623 AA; 67320 MW; BEADCE6A51CE3E1B CRC64;
METVGRFQAG DYVVFACLFV VSSGIGVFFA IKERNKAPSK EFLVGGRQMS CGPVALSLTA
SFMSAVTVIG APADVYRFGA SYVIFGVAYT FVVFFTAELF LPVFYRSGIT STYEYLELRF
CKLVRVAATL IYIIQTILYT GVVVYAPALA LNQVTGFDLW GSIFATGIVC TFYCTLGGLK
AVVWTDAFQM VVMVVGFLTV LIQGSSRAGG IENVWSTSRT GGRLQVFDFD VSPLRRHTFW
TLSVGGTFTW LGIYGVNQST IQRCISCKTE GHARWALYLN LLGLWIILFC AVVSGLIMYS
YYSHCDPWSS GLISAPDQLM PYFVMEILGA FPGLPGLFVA CAFSGTLSTV AASINALATV
MYEDFVSQCF PDLSNRAASW ISKALCVAFG VACTTMAVAA SYMGGIVQAA LSIHGMCGGP
VLGLFSLGIL FPFTNLKGAV GGLIVGISLS FWVGVGAFIY PAPSNNTHAL ELNTAGCNIT
AAAFEPTSAT VTQLTSDRNW LADSWYSMSY LYYSAVGFIG TVAAGLLITL LTGPMDPKLL
KPGMTRSVKE VMCFCTEKFT EADLGEGKED VGDFGKAWEK HPDQGCTLRM DEKFRCSCDN
QQENGNTNAG FDHNETSIVQ KKL
