ID   SC61A_WICAO             Reviewed;         479 AA.
AC   Q96TW8;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Protein transport protein SEC61 subunit alpha;
GN   Name=SEC61;
OS   Wickerhamomyces anomalus (Yeast) (Hansenula anomala).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX   NCBI_TaxID=4927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 36904 / CBS 1982 / NCYC 435 / NRRL Y-2153-4;
RX   PubMed=11561286; DOI=10.1002/yea.766;
RA   Ruiz T., Sanchez M., de la Rosa J.M., Rodriguez L., Dominguez A.;
RT   "The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61
RT   and FBP1 genes and five new open reading frames.";
RL   Yeast 18:1187-1195(2001).
CC   -!- FUNCTION: Appears to play a crucial role in the insertion of secretory
CC       and membrane polypeptides into the ER. It is required for assembly of
CC       membrane and secretory proteins and is essential for cell growth. It
CC       interacts with other membrane proteins required for protein
CC       translocation. Upon binding to SEC62/63 complex, secretory precursor
CC       polypeptides may engage SEC61 to begin membrane penetration event. A
CC       cycle of assembly and disassembly of SEC62/63 from SEC61 may govern the
CC       activity of the translocase (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and
CC       SEC61-gamma. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the SecY/SEC61-alpha family. {ECO:0000305}.
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DR   EMBL; AJ306295; CAC69141.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q96TW8; -.
DR   SMR; Q96TW8; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3370.10; -; 1.
DR   InterPro; IPR002208; SecY/SEC61-alpha.
DR   InterPro; IPR030659; SecY_CS.
DR   InterPro; IPR023201; SecY_dom_sf.
DR   InterPro; IPR019561; Translocon_Sec61/SecY_plug_dom.
DR   PANTHER; PTHR10906; PTHR10906; 1.
DR   Pfam; PF10559; Plug_translocon; 1.
DR   Pfam; PF00344; SecY; 1.
DR   PIRSF; PIRSF004557; SecY; 1.
DR   SUPFAM; SSF103491; SSF103491; 1.
DR   TIGRFAMs; TIGR00967; 3a0501s007; 1.
DR   PROSITE; PS00755; SECY_1; 1.
DR   PROSITE; PS00756; SECY_2; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Membrane; Protein transport; Translocation;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..479
FT                   /note="Protein transport protein SEC61 subunit alpha"
FT                   /id="PRO_0000131784"
FT   TOPO_DOM        1..33
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        55..76
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        98..119
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        141..146
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        168..246
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        268..361
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        383..415
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..434
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        435..440
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        441..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        459..479
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   479 AA;  52972 MW;  9B086AA0E89E46BE CRC64;
     MSSFRVLDLV KPLTGYLPEV IAPERKVPFN QKLMWTGVTL LIFLVMSEIP LYGIVSSESS
     DPLFWLRMML ASNRGTLMEL GISPIVSSGM VFQLLQGTQL LDVNLESKTD RETFQTAQKL
     FAILLSIGQA TVYVLTGIYG RPSDLGVGVC LLLILQLVFA GIIVILLDEL LQKGYGLGSG
     ISLFMATNIC EQIFWKAFAP TTVNNGRGDE FEGAVVALFH LLSVRKDKRR ALVEAFYRQN
     LPNIFQLLAT FIVFFLVVYL QGFRYEIPVR STRQRGQNGL YPIKLFYTSN TPIMLQSALT
     SNFFIISQML FQRFPLNPVV RLFGVWDARP GSAQLFASNG LAYYIQPPLS LTEALLDPIK
     TVIYVSFVLS VCALFSKTWI EISGTAPRDV AKQFKDQGLV IAGRRETSVY KELKRIIPTA
     AAFGGASIGA LSVACDLLGT LGSGTSILLA VTTIYSYYEI AAKEGGFQKH IVEGFSEAF