SC6A4_DROME
ID SC6A4_DROME Reviewed; 622 AA.
AC P51905; Q23969; Q9W177;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Sodium-dependent serotonin transporter;
DE AltName: Full=5HT transporter;
DE Short=5HTT;
DE AltName: Full=Cocaine-sensitive serotonin transporter;
DE AltName: Full=dSERT1;
GN Name=SerT; ORFNames=CG4545;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Head;
RX PubMed=8197200; DOI=10.1073/pnas.91.11.5158;
RA Demchyshyn L.L., Pristupa Z.B., Sugamori K.S., Barker E.L., Blakely R.D.,
RA Wolfgang W.J., Forte M.A., Niznik H.B.;
RT "Cloning, expression, and localization of a chloride-facilitated, cocaine-
RT sensitive serotonin transporter from Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:5158-5162(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-622, FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Head;
RX PubMed=8302852; DOI=10.1073/pnas.91.3.1188;
RA Corey J.L., Quick M.W., Davidson N., Lester H.A., Guastella J.;
RT "A cocaine-sensitive Drosophila serotonin transporter: cloning, expression,
RT and electrophysiological characterization.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:1188-1192(1994).
CC -!- FUNCTION: Terminates the action of serotonin by its high affinity
CC sodium-dependent reuptake into presynaptic terminals.
CC {ECO:0000269|PubMed:8197200, ECO:0000269|PubMed:8302852}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8197200,
CC ECO:0000269|PubMed:8302852}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q7K4Y6}.
CC -!- TISSUE SPECIFICITY: Expression is specific to cell bodies in the
CC ventral ganglion of the embryonic and larval nervous system.
CC {ECO:0000269|PubMed:8197200, ECO:0000269|PubMed:8302852}.
CC -!- MISCELLANEOUS: This protein is the target of psychomotor stimulants
CC such as amphetamines or cocaine.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10615.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U04809; AAA19430.1; -; mRNA.
DR EMBL; AE013599; AAF47200.1; -; Genomic_DNA.
DR EMBL; AY071023; AAL48645.1; -; mRNA.
DR EMBL; U02296; AAD10615.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001286839.1; NM_001299910.1.
DR RefSeq; NP_523846.2; NM_079122.3.
DR AlphaFoldDB; P51905; -.
DR SMR; P51905; -.
DR STRING; 7227.FBpp0072269; -.
DR TCDB; 2.A.22.1.9; the neurotransmitter:sodium symporter (nss) family.
DR GlyGen; P51905; 1 site.
DR PaxDb; P51905; -.
DR PRIDE; P51905; -.
DR DNASU; 37895; -.
DR EnsemblMetazoa; FBtr0072362; FBpp0072269; FBgn0010414.
DR EnsemblMetazoa; FBtr0343316; FBpp0309974; FBgn0010414.
DR GeneID; 37895; -.
DR KEGG; dme:Dmel_CG4545; -.
DR CTD; 37895; -.
DR FlyBase; FBgn0010414; SerT.
DR VEuPathDB; VectorBase:FBgn0010414; -.
DR eggNOG; KOG3659; Eukaryota.
DR GeneTree; ENSGT00940000169003; -.
DR HOGENOM; CLU_006855_9_0_1; -.
DR InParanoid; P51905; -.
DR OMA; CSSVMCI; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; P51905; -.
DR Reactome; R-DME-380615; Serotonin clearance from the synaptic cleft.
DR BioGRID-ORCS; 37895; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37895; -.
DR PRO; PR:P51905; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0010414; Expressed in neuron and 34 other tissues.
DR ExpressionAtlas; P51905; baseline and differential.
DR Genevisible; P51905; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0043679; C:axon terminus; IDA:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008504; F:monoamine transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0051378; F:serotonin binding; IBA:GO_Central.
DR GO; GO:0005335; F:serotonin:sodium symporter activity; IDA:FlyBase.
DR GO; GO:0015844; P:monoamine transport; IDA:FlyBase.
DR GO; GO:0006837; P:serotonin transport; IDA:FlyBase.
DR GO; GO:0051610; P:serotonin uptake; IDA:FlyBase.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Membrane; Metal-binding;
KW Neurotransmitter transport; Reference proteome; Sodium; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..622
FT /note="Sodium-dependent serotonin transporter"
FT /id="PRO_0000214761"
FT TOPO_DOM 1..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..130
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..244
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..263
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..289
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..342
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..375
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..427
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..473
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..549
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 568..586
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..622
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 89
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 91
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 92
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 96
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 328
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 360
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 425
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 428
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT BINDING 429
FT /ligand="Na(+)"
FT /ligand_id="ChEBI:CHEBI:29101"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 195..204
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
SQ SEQUENCE 622 AA; 69326 MW; 8E100BA90905A98D CRC64;
MDRSGSSDFA GAAATTGRSN PAPWSDDKES PNNEDDSNED DGDHTTPAKV TDPLAPKLAN
NERILVVSVT ERTRETWGQK AEFLLAVIGF AVDLGNVWRF PYICYQNGGG AFLVPYCLFL
IFGGLPLFYM ELALGQFHRC GCLSIWKRIC PALKGVGYAI CLIDIYMGMY YNTIIGWAVY
YLFASFTSKL PWTSCDNPWN TENCMQVTSE NFTELATSPA KEFFERKVLE SYKGNGLDFM
GPVKPTLALC VFGVFVLVYF SLWKGVRSAG KVVWVTALAP YVVLIILLVR GVSLPGADEG
IKYYLTPEWH KLKNSKVWID AASQIFFSLG PGFGTLLALS SYNKFNNNCY RDALITSSIN
CLTSFLAGFV IFSVLGYMAY VQKTSIDKVG LEGPGLVFIV YPEAIATMSG SVFWSIIFFL
MLITLGLDST FGGLEAMITA LCDEYPRVIG RRRELFVLLL LAFIFLCALP TMTYGGVVLV
NFLNVYGPGL AILFVVFVEA AGVFWFYGVD RFSSDVEQML GSKPGLFWRI CWTYISPVFL
LTIFIFSIMG YKEMLGEEYY YPDWSYQVGW AVTCSSVLCI PMYIIYKFFF ASKGGCRQRL
QESFQPEDNC GSVVPGQQGT SV
