SC6A7_MOUSE
ID SC6A7_MOUSE Reviewed; 637 AA.
AC Q6PGE7; Q80UM1;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Sodium-dependent proline transporter;
DE AltName: Full=Solute carrier family 6 member 7;
GN Name=Slc6a7 {ECO:0000312|EMBL:AAH57070.1, ECO:0000312|MGI:MGI:2147363};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:AAH57070.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH57070.1}, and
RC ICR {ECO:0000312|EMBL:AAH50103.1};
RC TISSUE=Brain {ECO:0000312|EMBL:AAH57070.1}, and Trophoblast stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-22; SER-598 AND
RP SER-600, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Brain specific sodium (and chloride)-dependent proline
CC transporter. Terminates the action of proline by its high affinity
CC sodium-dependent reuptake into presynaptic terminals.
CC {ECO:0000250|UniProtKB:P28573}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + L-proline(out) + 2 Na(+)(out) = chloride(in) +
CC L-proline(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71263, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC Evidence={ECO:0000250|UniProtKB:P28573};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + L-pipecolate(out) + 2 Na(+)(out) =
CC chloride(in) + L-pipecolate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71267,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:61185;
CC Evidence={ECO:0000250|UniProtKB:P28573};
CC -!- SUBCELLULAR LOCATION: Synaptic cell membrane
CC {ECO:0000250|UniProtKB:Q99884}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A7 subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC050103; AAH50103.1; -; mRNA.
DR EMBL; BC057070; AAH57070.1; -; mRNA.
DR CCDS; CCDS29278.1; -.
DR RefSeq; NP_958741.1; NM_201353.1.
DR AlphaFoldDB; Q6PGE7; -.
DR SMR; Q6PGE7; -.
DR STRING; 10090.ENSMUSP00000025520; -.
DR GlyGen; Q6PGE7; 1 site.
DR iPTMnet; Q6PGE7; -.
DR PhosphoSitePlus; Q6PGE7; -.
DR PaxDb; Q6PGE7; -.
DR PeptideAtlas; Q6PGE7; -.
DR PRIDE; Q6PGE7; -.
DR ProteomicsDB; 256739; -.
DR Antibodypedia; 27933; 84 antibodies from 17 providers.
DR DNASU; 240332; -.
DR Ensembl; ENSMUST00000025520; ENSMUSP00000025520; ENSMUSG00000052026.
DR GeneID; 240332; -.
DR KEGG; mmu:240332; -.
DR UCSC; uc008fbi.1; mouse.
DR CTD; 6534; -.
DR MGI; MGI:2147363; Slc6a7.
DR VEuPathDB; HostDB:ENSMUSG00000052026; -.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00940000160823; -.
DR HOGENOM; CLU_006855_9_4_1; -.
DR InParanoid; Q6PGE7; -.
DR OMA; REEGTLW; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; Q6PGE7; -.
DR TreeFam; TF343812; -.
DR Reactome; R-MMU-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-MMU-71288; Creatine metabolism.
DR BioGRID-ORCS; 240332; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Slc6a7; mouse.
DR PRO; PR:Q6PGE7; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q6PGE7; protein.
DR Bgee; ENSMUSG00000052026; Expressed in subiculum and 101 other tissues.
DR Genevisible; Q6PGE7; MM.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099056; C:integral component of presynaptic membrane; ISO:MGI.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; ISO:MGI.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; ISO:MGI.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; IDA:MGI.
DR GO; GO:0005298; F:proline:sodium symporter activity; ISS:UniProtKB.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0035524; P:proline transmembrane transport; IDA:MGI.
DR GO; GO:0015824; P:proline transport; ISS:UniProtKB.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Glycoprotein; Membrane;
KW Neurotransmitter transport; Phosphoprotein; Reference proteome; Symport;
KW Synapse; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..637
FT /note="Sodium-dependent proline transporter"
FT /id="PRO_0000214771"
FT TOPO_DOM 1..45
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..93
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..214
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..233
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..259
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..312
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..345
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..397
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..443
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 500..519
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..556
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..637
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 20
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 573
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P28573"
FT MOD_RES 582
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P28573"
FT MOD_RES 588
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P28573"
FT MOD_RES 591
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P28573"
FT MOD_RES 598
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 600
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 21
FT /note="P -> S (in Ref. 1; AAH50103)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="Q -> R (in Ref. 1; AAH50103)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 637 AA; 71066 MW; A122D5A6ED074680 CRC64;
MKKLQEAHLR KPITPDLLMT PSDQGDVDLD VDFAADRGNW TGKLDFLLSC IGYCVGLGNV
WRFPYRAYTN GGGAFLVPYF LMLAICGIPL FFLELSLGQF SSLGPLAVWK ISPLFKGAGA
AMLLIVGLVA IYYNMIIAYV LFYLFASLTS NLPWEHCGNW WNTELCLEHR GPKSGNGVLP
LNLSSTVSPS EEYWSRYVLH IQGSQGIGRP GEIRWNLCLC LLLAWVIVFL CILKGVKSSG
KVVYFTATFP YLILLMLLVR GVTLPGAWKG IQFYLTPQFH HLLSSKVWIE AALQIFYSLG
VGFGGLLTFA SYNTFHQNIY RDTFIVTLGN AITSILAGFA IFSVLGYMSQ ELGVPVDQVA
KAGPGLAFVV YPQAMTMLPL SPFWSFLFFF MLLTLGLDSQ FAFLETIVTA VTDEFPYYLR
PKKAVFSGLI CVAMYLMGLI LTTDGGMYWL VLLDDYSASF GLMVVVITTC LAVTRVYGIQ
RFCRDIHMML GFKPGLYFRA CWLFLSPATL LALLVYSIVK YQPSEYGSYR FPAWAELLGI
LMGLLSCLMI PAGMLVAVLR EEGSLWERLQ QASRPAMDWG PSLEENRTGM YVATLAGSQS
PKPLMVHMRK YGGITSFENT AIEVDREIAE EEEESMM
