SC6A8_MOUSE
ID SC6A8_MOUSE Reviewed; 640 AA.
AC Q8VBW1; A2ALM4; Q80YC9; Q8K4R3; Q8R1L0;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Sodium- and chloride-dependent creatine transporter 1;
DE Short=CT1;
DE Short=Creatine transporter 1;
DE AltName: Full=Solute carrier family 6 member 8;
GN Name=Slc6a8; Synonyms=Crt;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=12439290; DOI=10.1097/01.wcb.0000033966.83623.7d;
RA Ohtsuki S., Tachikawa M., Takanaga H., Shimizu H., Watanabe M., Hosoya K.,
RA Terasaki T.;
RT "The blood-brain barrier creatine transporter is a major pathway for
RT supplying creatine to the brain.";
RL J. Cereb. Blood Flow Metab. 22:1327-1335(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1).
RA Liu Q.-R., Li Q.-F.;
RT "Cloning and expression of mouse creatine transporter gene.";
RL Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-640 (ISOFORMS 2 AND 3).
RC STRAIN=FVB/N; TISSUE=Embryo, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-625 AND SER-628, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for the uptake of creatine. Plays an important role
CC in supplying creatine to the brain via the blood-brain barrier.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8VBW1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VBW1-2; Sequence=VSP_012133;
CC Name=3;
CC IsoId=Q8VBW1-3; Sequence=VSP_012132;
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A8 subfamily. {ECO:0000305}.
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DR EMBL; AB077327; BAC11857.1; -; mRNA.
DR EMBL; AF459435; AAL66354.1; -; mRNA.
DR EMBL; AF459436; AAL66355.1; -; Genomic_DNA.
DR EMBL; AL805924; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC024444; AAH24444.1; -; mRNA.
DR EMBL; BC049801; AAH49801.1; -; mRNA.
DR CCDS; CCDS30208.1; -. [Q8VBW1-1]
DR CCDS; CCDS53098.1; -. [Q8VBW1-2]
DR RefSeq; NP_001136281.1; NM_001142809.1. [Q8VBW1-2]
DR RefSeq; NP_001136282.1; NM_001142810.1. [Q8VBW1-3]
DR RefSeq; NP_598748.1; NM_133987.2. [Q8VBW1-1]
DR AlphaFoldDB; Q8VBW1; -.
DR SMR; Q8VBW1; -.
DR BioGRID; 221964; 6.
DR IntAct; Q8VBW1; 6.
DR STRING; 10090.ENSMUSP00000033752; -.
DR GlyGen; Q8VBW1; 3 sites.
DR iPTMnet; Q8VBW1; -.
DR PhosphoSitePlus; Q8VBW1; -.
DR jPOST; Q8VBW1; -.
DR MaxQB; Q8VBW1; -.
DR PaxDb; Q8VBW1; -.
DR PRIDE; Q8VBW1; -.
DR ProteomicsDB; 255475; -. [Q8VBW1-1]
DR ProteomicsDB; 255476; -. [Q8VBW1-2]
DR ProteomicsDB; 255477; -. [Q8VBW1-3]
DR DNASU; 102857; -.
DR Ensembl; ENSMUST00000033752; ENSMUSP00000033752; ENSMUSG00000019558. [Q8VBW1-1]
DR Ensembl; ENSMUST00000114465; ENSMUSP00000110109; ENSMUSG00000019558. [Q8VBW1-2]
DR Ensembl; ENSMUST00000114467; ENSMUSP00000110111; ENSMUSG00000019558. [Q8VBW1-2]
DR GeneID; 102857; -.
DR KEGG; mmu:102857; -.
DR UCSC; uc009tmf.2; mouse. [Q8VBW1-1]
DR UCSC; uc009tmg.2; mouse. [Q8VBW1-3]
DR CTD; 6535; -.
DR MGI; MGI:2147834; Slc6a8.
DR VEuPathDB; HostDB:ENSMUSG00000019558; -.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00940000155869; -.
DR InParanoid; Q8VBW1; -.
DR OMA; CVEIFRQ; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; Q8VBW1; -.
DR TreeFam; TF343812; -.
DR Reactome; R-MMU-71288; Creatine metabolism.
DR BioGRID-ORCS; 102857; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Slc6a8; mouse.
DR PRO; PR:Q8VBW1; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8VBW1; protein.
DR Bgee; ENSMUSG00000019558; Expressed in small intestine Peyer's patch and 255 other tissues.
DR ExpressionAtlas; Q8VBW1; baseline and differential.
DR Genevisible; Q8VBW1; MM.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; ISO:MGI.
DR GO; GO:0015220; F:choline transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005308; F:creatine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005309; F:creatine:sodium symporter activity; ISO:MGI.
DR GO; GO:0015881; P:creatine transmembrane transport; ISS:UniProtKB.
DR GO; GO:1990403; P:embryonic brain development; IEA:Ensembl.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:InterPro.
DR GO; GO:0071705; P:nitrogen compound transport; ISO:MGI.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002984; Na/ntran_symport_creatine.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01199; CRTTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Ion transport; Membrane;
KW Phosphoprotein; Reference proteome; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..640
FT /note="Sodium- and chloride-dependent creatine transporter
FT 1"
FT /id="PRO_0000214775"
FT TOPO_DOM 1..60
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..87
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..304
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..394
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..484
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 506..525
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 547..565
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 587..640
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 622
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P48029"
FT MOD_RES 625
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 628
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 192
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 553
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 462..469
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_012132"
FT VAR_SEQ 465..469
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12439290,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_012133"
SQ SEQUENCE 640 AA; 70999 MW; 67D3AAAC591EEA0B CRC64;
MAKKSAENGI YSVSGDEKKG PLIVSGPDGA PAKGDGPAGL GAPGGRLAVP PRETWTRQMD
FIMSCVGFAV GLGNVWRFPY LCYKNGGGVF LIPYVLIALV GGIPIFFLEI SLGQFMKAGS
INVWNICPLF KGLGYASMVI VFYCNTYYIM VLAWGFYYLV KSFTTTLPWA TCGHTWNTPD
CVEIFRHEDC ANASLANLTC DQLADRRSPV IEFWENKVLR LSTGLEVPGA LNWEVTLCLL
ACWVLVYFCV WKGVKSTGKI VYFTATFPYV VLVVLLVRGV LLPGALDGII YYLKPDWSKL
GSPQVWIDAG TQIFFSYAIG LGALTALGSY NRFNNNCYKD AIILALINSG TSFFAGFVVF
SILGFMATEQ GVHISKVAES GPGLAFIAYP RAVTLMPVAP LWAALFFFML LLLGLDSQFV
GVEGFITGLL DLLPASYYFR FQREISVALC CALCFVIDLS MVTDVSGGKG GMYVFQLFDY
YSASGTTLLW QAFWECVVVA WVYGADRFMD DIACMIGYRP CPWMKWCWSF FTPLVCMGIF
IFNIVYYEPL VYNNTYVYPW WGEAMGWAFA LSSMLCVPLH LLGCLLRAKG TMAERWQHLT
QPIWGLHHLE YRAQDADVRG LTTLTPVSES SKVVVVESVM
