SC6A8_RAT
ID SC6A8_RAT Reviewed; 635 AA.
AC P28570;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Sodium- and chloride-dependent creatine transporter 1;
DE Short=CHOT1;
DE Short=CT1;
DE Short=Creatine transporter 1;
DE AltName: Full=Solute carrier family 6 member 8;
GN Name=Slc6a8;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Brain;
RX PubMed=1633856; DOI=10.1016/0014-5793(92)80649-2;
RA Mayser W., Schloss P., Betz H.;
RT "Primary structure and functional expression of a choline transporter
RT expressed in the rat nervous system.";
RL FEBS Lett. 305:31-36(1992).
CC -!- FUNCTION: Required for the uptake of creatine. Plays an important role
CC in supplying creatine to the brain via the blood-brain barrier (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in brain, cerebellum, spinal cord, to a
CC lesser extent, heart, but only very low expression in lung, kidney, and
CC muscle.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A8 subfamily. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a choline transporter.
CC {ECO:0000305|PubMed:1633856}.
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DR EMBL; X66494; CAA47119.1; -; mRNA.
DR PIR; S23431; S23431.
DR RefSeq; NP_059044.1; NM_017348.2.
DR AlphaFoldDB; P28570; -.
DR SMR; P28570; -.
DR STRING; 10116.ENSRNOP00000025702; -.
DR BindingDB; P28570; -.
DR ChEMBL; CHEMBL3814; -.
DR DrugCentral; P28570; -.
DR TCDB; 2.A.22.3.5; the neurotransmitter:sodium symporter (nss) family.
DR GlyGen; P28570; 3 sites.
DR iPTMnet; P28570; -.
DR PhosphoSitePlus; P28570; -.
DR PaxDb; P28570; -.
DR PRIDE; P28570; -.
DR GeneID; 50690; -.
DR KEGG; rno:50690; -.
DR CTD; 6535; -.
DR RGD; 619711; Slc6a8.
DR VEuPathDB; HostDB:ENSRNOG00000057620; -.
DR eggNOG; KOG3660; Eukaryota.
DR HOGENOM; CLU_006855_9_5_1; -.
DR InParanoid; P28570; -.
DR OMA; CVEIFRQ; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; P28570; -.
DR Reactome; R-RNO-71288; Creatine metabolism.
DR PRO; PR:P28570; -.
DR Proteomes; UP000002494; Chromosome X.
DR Bgee; ENSRNOG00000057620; Expressed in jejunum and 19 other tissues.
DR ExpressionAtlas; P28570; baseline and differential.
DR Genevisible; P28570; RN.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IDA:SynGO.
DR GO; GO:0005886; C:plasma membrane; TAS:RGD.
DR GO; GO:0015220; F:choline transmembrane transporter activity; IDA:RGD.
DR GO; GO:0005308; F:creatine transmembrane transporter activity; IDA:RGD.
DR GO; GO:0005309; F:creatine:sodium symporter activity; IDA:RGD.
DR GO; GO:0015881; P:creatine transmembrane transport; IDA:RGD.
DR GO; GO:1990403; P:embryonic brain development; IEP:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:InterPro.
DR GO; GO:0071705; P:nitrogen compound transport; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002984; Na/ntran_symport_creatine.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01199; CRTTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..635
FT /note="Sodium- and chloride-dependent creatine transporter
FT 1"
FT /id="PRO_0000214777"
FT TOPO_DOM 1..60
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..87
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..230
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..304
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..394
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..465
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 466..479
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 501..520
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 542..560
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 582..635
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 617
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P48029"
FT MOD_RES 620
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P48029"
FT MOD_RES 623
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VBW1"
FT CARBOHYD 192
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 548
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 635 AA; 70632 MW; 5D6A944035103787 CRC64;
MAKKSAENGI YSVSGDEKKG PLIVSGPDGA PSKGDGPAGL GAPSSRLAVP PRETWTRQMD
FIMSCVGFAV GLGNVWRFPY LCYKNGGGVF LIPYVLIALV GGIPIFFLEI SLGQFMKAGS
INVWNICPLF KGLGYASMVI VFYCNTYYIM VLAWGFYYLV KSFTTTLPWA TCGHTWNTPD
CVEIFRHEDC ANASLANLTC DQLADRRSPV IEFWENKVLR LSTGLEVPGA LNWEVTLCLL
ACWVLVYFCV WKGVKSTGKI VYFTATFPYV VLVVLLVRGV LLPGALDGII YYLKPDWSKL
GSPQVWIDAG TQIFFSYAIG LGALTALGSY NRFNNNCYKD AIILALINSG TSFFAGFVVF
SILGFMATEQ GVHISKVAES GPGLAFIAYP RAVTLMPVAP LWAALFFFML LLLGLDSQFV
GVEGFITGLL DLLPASYYFR FQREISVALC CALCFVIDLS MVTDGGMYVF QLFDYYSASG
TTLLWQAFWE CVVVAWVYGA DRFMDDIACM IGYRPCPWMK WCWSFFTPLV CMGIFIFNVV
YYKPLVYNNT YVYPWWGEAM GWAFALSSML CVPLHLLGCL LRAKGTMAER WQHLTQPIWG
LHHLEYRAQD ADVRGLTTLT PVSESSKVVV VESVM
