SC6A9_BOVIN
ID SC6A9_BOVIN Reviewed; 638 AA.
AC Q28039; Q28040; Q29420;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Sodium- and chloride-dependent glycine transporter 1;
DE Short=GlyT-1;
DE Short=GlyT1;
DE AltName: Full=Solute carrier family 6 member 9;
GN Name=SLC6A9;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
RA Jones E.M.C.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sodium- and chloride-dependent glycine transporter which is
CC essential for regulating glycine concentrations at inhibitory
CC glycinergic synapses. {ECO:0000250|UniProtKB:P28571}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + glycine(out) + 2 Na(+)(out) = chloride(in) +
CC glycine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:70691, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:57305;
CC Evidence={ECO:0000250|UniProtKB:P28571};
CC -!- SUBUNIT: Interacts with EXOC1; interaction increases the transporter
CC capacity of SLC6A9 probably by promoting its insertion into the cell
CC membrane (By similarity). Interacts with EXOC3 and EXOC4 (By
CC similarity). {ECO:0000250|UniProtKB:P28572}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P28572};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=GlyT-1B;
CC IsoId=Q28039-1; Sequence=Displayed;
CC Name=2; Synonyms=GlyT-1A;
CC IsoId=Q28039-2; Sequence=VSP_006268;
CC Name=3; Synonyms=GlyT-1F;
CC IsoId=Q28039-3; Sequence=VSP_006269;
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A9 subfamily. {ECO:0000305}.
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DR EMBL; U52687; AAB01158.1; -; mRNA.
DR EMBL; U52688; AAB01159.1; -; mRNA.
DR EMBL; U52689; AAB01160.1; -; mRNA.
DR EMBL; U52690; AAB01161.1; -; mRNA.
DR RefSeq; NP_001229271.1; NM_001242342.1.
DR RefSeq; NP_001229272.1; NM_001242343.1.
DR AlphaFoldDB; Q28039; -.
DR SMR; Q28039; -.
DR STRING; 9913.ENSBTAP00000007938; -.
DR BindingDB; Q28039; -.
DR ChEMBL; CHEMBL3638333; -.
DR PaxDb; Q28039; -.
DR PRIDE; Q28039; -.
DR GeneID; 282368; -.
DR KEGG; bta:282368; -.
DR CTD; 6536; -.
DR eggNOG; KOG3660; Eukaryota.
DR InParanoid; Q28039; -.
DR OrthoDB; 250396at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015375; F:glycine:sodium symporter activity; ISS:UniProtKB.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0060092; P:regulation of synaptic transmission, glycinergic; ISS:UniProtKB.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR003028; Na/ntran_symport_glycine_GLY1.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01204; GLY1TRNSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Cell membrane; Glycoprotein;
KW Membrane; Neurotransmitter transport; Phosphoprotein; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..638
FT /note="Sodium- and chloride-dependent glycine transporter
FT 1"
FT /id="PRO_0000214779"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..219
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..238
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..264
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..317
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..350
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..402
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..449
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..525
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 544..562
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 563..638
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 597..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 627..638
FT /note="Essential for interaction with EXOC1"
FT /evidence="ECO:0000250|UniProtKB:P28572"
FT COMPBIAS 9..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 624..638
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 603
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P28571"
FT MOD_RES 605
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P28571"
FT MOD_RES 630
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P48067"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 172
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 188
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..14
FT /note="MAAAQGPVAPSSLE -> MVGKGAKGM (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_006268"
FT VAR_SEQ 537..638
FT /note="YPSWRVRIGFLMALSSVICIPLYALFQFCRTDGDTLLHRLKNATKPSRDWGP
FT ALLEHRTRRYAPTTTPSPEDGLEVQPLHPDKAQIPMVGSNGSSRFQDSRI -> SSQTG
FT LPLFTCQIAPAHVPQPLSGARTPSPKPWSVRVSVLRAPLCSDSPGRAASNPL (in
FT isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_006269"
SQ SEQUENCE 638 AA; 71318 MW; 97ADDBF28EC2A443 CRC64;
MAAAQGPVAP SSLEQNGAVP SEATKKDQNL KRGNWGNQIE FVLTSVGYAV GLGNVWRFPY
LCYRNGGGAF MFPYFIMLIF CGIPLFFMEL SFGQFASQGC LGVWRISPMF KGVGYGMMVV
STYIGIYYNV VICIAFYYFF SSMTPVLPWT YCNNPWNTPD CMSVLDNPNI TNGSQPPALP
GNVSQALNQT LKRTSPSEEY WRLYVLKLSD DIGNFGEVRL PLLGCLGVSW VVVFLCLIRG
VKSSGKVVYF TATFPYVVLT ILFIRGVTLE GAFTGIMYYL TPQWDKILEA KVWGDAASQI
FYSLGCAWGG LVTMASYNKF HNNCYRDSVI ISITNCATSV YAGFVIFSIL GFMANHLGVD
VSRVADHGPG LAFVAYPEAL TLLPISPLWS LLFFFMLILL GLGTQFCLLE TLVTAIVDEV
GNEWILQKKT YVTLGVAVAG FLLGIPLTSQ AGIYWLLLMD NYAASFSLVI ISCIMCVSIM
YIYGHQNYFQ DIQMMLGFPP PLFFQICWRF VSPAIIFFIL IFSVIQYQPI TYNQYQYPSW
RVRIGFLMAL SSVICIPLYA LFQFCRTDGD TLLHRLKNAT KPSRDWGPAL LEHRTRRYAP
TTTPSPEDGL EVQPLHPDKA QIPMVGSNGS SRFQDSRI
