ID   SC6A9_XENLA             Reviewed;         633 AA.
AC   A7Y2W8;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Sodium- and chloride-dependent glycine transporter 1;
DE            Short=GlyT-1;
DE            Short=GlyT1;
DE            Short=xGlyT1;
DE   AltName: Full=Solute carrier family 6 member 9;
GN   Name=slc6a9 {ECO:0000250|UniProtKB:P48067};
GN   Synonyms=glyt1 {ECO:0000312|EMBL:ABV03172.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ABV03172.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Tadpole {ECO:0000269|PubMed:18262473};
RX   PubMed=18262473; DOI=10.1016/j.gep.2007.12.005;
RA   Wester M.R., Teasley D.C., Byers S.L., Saha M.S.;
RT   "Expression patterns of glycine transporters (xGlyT1, xGlyT2, and xVIAAT)
RT   in Xenopus laevis during early development.";
RL   Gene Expr. Patterns 8:261-270(2008).
CC   -!- FUNCTION: Sodium- and chloride-dependent glycine transporter which is
CC       essential for regulating glycine concentrations at inhibitory
CC       glycinergic synapses. {ECO:0000250|UniProtKB:P28571}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chloride(out) + glycine(out) + 2 Na(+)(out) = chloride(in) +
CC         glycine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:70691, ChEBI:CHEBI:17996,
CC         ChEBI:CHEBI:29101, ChEBI:CHEBI:57305;
CC         Evidence={ECO:0000250|UniProtKB:P28571};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P28572};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: First expressed in early tailbud stage embryos in
CC       the midbrain and anterior spinal cord, and weakly in the hindbrain. By
CC       late tailbud stages, expression extends posteriorly in the spinal cord
CC       to appear in between somites. Expressed in the forebrain, retina,
CC       between the somites and in the blood islands by the swimming tadpole
CC       stages. {ECO:0000269|PubMed:18262473}.
CC   -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC       2.A.22) family. SLC6A9 subfamily. {ECO:0000305}.
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DR   EMBL; EU117185; ABV03172.1; -; mRNA.
DR   RefSeq; NP_001104228.1; NM_001110758.1.
DR   AlphaFoldDB; A7Y2W8; -.
DR   SMR; A7Y2W8; -.
DR   GeneID; 108714278; -.
DR   CTD; 108714278; -.
DR   Xenbase; XB-GENE-17338334; slc6a9.L.
DR   Proteomes; UP000186698; Genome assembly.
DR   Bgee; 108714278; Expressed in egg cell and 15 other tissues.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0015375; F:glycine:sodium symporter activity; ISS:UniProtKB.
DR   GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR   GO; GO:0060092; P:regulation of synaptic transmission, glycinergic; ISS:UniProtKB.
DR   InterPro; IPR000175; Na/ntran_symport.
DR   InterPro; IPR003028; Na/ntran_symport_glycine_GLY1.
DR   InterPro; IPR037272; SNS_sf.
DR   PANTHER; PTHR11616; PTHR11616; 1.
DR   Pfam; PF00209; SNF; 1.
DR   PRINTS; PR01204; GLY1TRNSPORT.
DR   PRINTS; PR00176; NANEUSMPORT.
DR   SUPFAM; SSF161070; SSF161070; 1.
DR   PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR   PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid transport; Cell membrane; Glycoprotein; Membrane;
KW   Neurotransmitter transport; Reference proteome; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..633
FT                   /note="Sodium- and chloride-dependent glycine transporter
FT                   1"
FT                   /id="PRO_0000341534"
FT   TOPO_DOM        1..30
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        134..208
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        283..303
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical; Name=8"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        429..449
FT                   /note="Helical; Name=9"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        453..473
FT                   /note="Helical; Name=10"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        493..513
FT                   /note="Helical; Name=11"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        533..553
FT                   /note="Helical; Name=12"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        554..633
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          588..633
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        588..604
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        616..633
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        158
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        164
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        173
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        179
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   633 AA;  71022 MW;  9B78B51B4B1E3A71 CRC64;
     MGLCVNGAVP SEATKKDENL KRGNWGNQIE FVLTSVGYAV GLGNVWRFPY LCYRNGGGAF
     MFPYFIMLIF CGIPLFFMEL SFGQFASQGC LGVWRVSPIF KGVGYGMMVV STYIGIYYNV
     VICIAFYYFF ASMNRVLPWT YCNNLWNTNN CAGVLSPNSS ASFNLSSQQN LLNLTLGLNQ
     TLKRTSPSEE YWRRHVLKIS EDIGDFGEVQ LPLLGCLGVS WLVVFLCLIR GVKSSGKVVY
     FTATFPYVVL TILFIRGITL EGAINGILYY LTPQWDKILH AMVWGDAASQ IFYSLGCAWG
     GLITMASYNK FHNNCYRDSI IISITNCATS VYAGFVIFSI LGFMATHLGV DVSEVADHGP
     GLAFVAYPEA LTLLPISPLW SILFFFMLIL LGLGTQFCLL ETLVTAVVDE IGNDWIIRWK
     TLVTLGVAII GFLLGIPLTT QAGIYWLLLM DNYAASFSLV IISCIMCIAV MYIYGHRKYF
     KDIEMMLGFP PPLFFQICWR FISPGIIFFI LIFTVIQYRP IQYNDYLYPD WAITIGFLMA
     LSSVICIPLY AIFKIWCSEG DTFLQRLKNA VKPSKDWGPA LQEHRTGRYA QMSSTRSESN
     PEAQPLNPEK MKEDLSLTIQ GSNGQAHTQD SKV