SC6B2_YEAST
ID SC6B2_YEAST Reviewed; 88 AA.
AC P52871; D3DLR8; Q53X22;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Protein transport protein SBH2;
DE AltName: Full=Ssh1 complex subunit SBH2;
DE AltName: Full=Ssh1 complex subunit beta;
GN Name=SBH2; Synonyms=SEB2; OrderedLocusNames=YER019C-A; ORFNames=YER019BC;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8740416;
RX DOI=10.1002/(sici)1097-0061(199604)12:5<425::aid-yea924>3.0.co;2-b;
RA Toikkanen J., Gatti E., Takei K., Saloheimo M., Olkkonen V.M.,
RA Soederlund H., de Camilli P., Keraenen S.;
RT "Yeast protein translocation complex: isolation of two genes SEB1 and SEB2
RT encoding proteins homologous to the Sec61 beta subunit.";
RL Yeast 12:425-438(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Finke K., Panzner S., Sommer T., Hartmann E., Rapoport T.A.;
RT "Yeast Sec61 beta subunit.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP PROTEIN SEQUENCE OF 16-32.
RX PubMed=8612571; DOI=10.1002/j.1460-2075.1996.tb00492.x;
RA Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E.,
RA Sommer T.;
RT "A second trimeric complex containing homologs of the Sec61p complex
RT functions in protein transport across the ER membrane of S. cerevisiae.";
RL EMBO J. 15:1482-1494(1996).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Part of the Ssh1 complex, which probably is the major
CC component of a channel-forming translocon complex that may function
CC exclusively in the cotranslational pathway of protein endoplasmic
CC reticulum (ER) import.
CC -!- SUBUNIT: Component of the heterotrimeric Ssh1 complex, which is
CC composed of SSH1, SBH2 and SSS1.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC membrane protein.
CC -!- MISCELLANEOUS: Present with 414 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SEC61-beta family. {ECO:0000305}.
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DR EMBL; Z50012; CAA90305.1; -; mRNA.
DR EMBL; L38891; AAT01101.1; -; mRNA.
DR EMBL; U18778; AAB64567.1; -; Genomic_DNA.
DR EMBL; AY693199; AAT93218.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07672.1; -; Genomic_DNA.
DR PIR; S68163; S68163.
DR RefSeq; NP_010936.1; NM_001180031.1.
DR PDB; 2WW9; EM; 8.60 A; C=1-87.
DR PDB; 2WWA; EM; 8.90 A; C=1-87.
DR PDBsum; 2WW9; -.
DR PDBsum; 2WWA; -.
DR AlphaFoldDB; P52871; -.
DR SMR; P52871; -.
DR BioGRID; 36753; 74.
DR ComplexPortal; CPX-1834; SSH1 translocon complex.
DR DIP; DIP-1708N; -.
DR IntAct; P52871; 6.
DR MINT; P52871; -.
DR STRING; 4932.YER019C-A; -.
DR TCDB; 3.A.5.8.1; the general secretory pathway (sec) family.
DR MaxQB; P52871; -.
DR PaxDb; P52871; -.
DR PRIDE; P52871; -.
DR TopDownProteomics; P52871; -.
DR EnsemblFungi; YER019C-A_mRNA; YER019C-A; YER019C-A.
DR GeneID; 856740; -.
DR KEGG; sce:YER019C-A; -.
DR SGD; S000002127; SBH2.
DR VEuPathDB; FungiDB:YER019C-A; -.
DR eggNOG; KOG3457; Eukaryota.
DR GeneTree; ENSGT00940000176828; -.
DR HOGENOM; CLU_133423_1_1_1; -.
DR InParanoid; P52871; -.
DR OMA; FITCIMA; -.
DR BioCyc; YEAST:G3O-30348-MON; -.
DR Reactome; R-SCE-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR EvolutionaryTrace; P52871; -.
DR PRO; PR:P52871; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P52871; protein.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IC:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005637; C:nuclear inner membrane; IDA:SGD.
DR GO; GO:0005784; C:Sec61 translocon complex; IEA:InterPro.
DR GO; GO:0071261; C:Ssh1 translocon complex; IDA:SGD.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD.
DR GO; GO:0031204; P:post-translational protein targeting to membrane, translocation; IBA:GO_Central.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IGI:SGD.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR InterPro; IPR030671; Sec61-beta/Sbh.
DR InterPro; IPR016482; SecG/Sec61-beta/Sbh.
DR PANTHER; PTHR13509; PTHR13509; 1.
DR Pfam; PF03911; Sec61_beta; 1.
DR PIRSF; PIRSF006398; Sec61_beta_euk; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Endoplasmic reticulum; Membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..88
FT /note="Protein transport protein SBH2"
FT /id="PRO_0000157262"
FT TOPO_DOM 1..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 88 AA; 9606 MW; 5A4F5B9184202E88 CRC64;
MAASVPPGGQ RILQKRRQAQ SIKEKQAKQT PTSTRQAGYG GSSSSILKLY TDEANGFRVD
SLVVLFLSVG FIFSVIALHL LTKFTHII
