ID   SCAF_BPT4               Reviewed;         269 AA.
AC   P04534; P03721; P03722; Q38389;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   29-SEP-2021, entry version 84.
DE   RecName: Full=Capsid assembly scaffolding protein;
DE   AltName: Full=Gene product 22;
DE            Short=gp22;
DE   AltName: Full=Head morphogenesis protein {ECO:0000305};
DE   AltName: Full=Scaffold protein;
DE   Contains:
DE     RecName: Full=Internal peptide VII;
GN   Name=22;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=D;
RX   PubMed=2587226; DOI=10.1093/nar/17.21.8865;
RA   Marusich E.I., Mesyanzhinov V.V.;
RT   "Nucleotide and deduced amino acid sequences of bacteriophage T4 gene 22.";
RL   Nucleic Acids Res. 17:8865-8865(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 86-269.
RA   Christensen A.C.;
RL   Submitted (AUG-1981) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183.
RA   Noguchi T., Takahashi H.;
RL   Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 221-238.
RX   PubMed=6287296; DOI=10.1038/299369a0;
RA   Christensen A.C., Young E.T.;
RT   "T4 late transcripts are initiated near a conserved DNA sequence.";
RL   Nature 299:369-371(1982).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-239.
RX   PubMed=6692468; DOI=10.1016/0092-8674(84)90091-6;
RA   Elliott T., Geiduschek E.P.;
RT   "Defining a bacteriophage T4 late promoter: absence of a '-35' region.";
RL   Cell 36:211-219(1984).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 197-269.
RX   PubMed=6335532; DOI=10.1016/0022-2836(84)90019-6;
RA   Parker M.L., Christensen A.C., Boosman A., Stockard J., Young E.T.,
RA   Doermann A.H.;
RT   "Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid
RT   sequence of its product.";
RL   J. Mol. Biol. 180:399-416(1984).
RN   [8]
RP   PROTEIN SEQUENCE OF 62-84.
RX   PubMed=604510; DOI=10.1016/0022-2836(77)90141-3;
RA   van Eerd J.P., Champe S.P., Yager L., Kubota I., Tsugita A.;
RT   "Primary structure of internal peptide VII of T-even bacteriophages.";
RL   J. Mol. Biol. 117:521-524(1977).
RN   [9]
RP   FUNCTION (INTERNAL PEPTIDE VII), AND FUNCTION (CAPSID ASSEMBLY SCAFFOLDING
RP   PROTEIN).
RX   PubMed=6382791; DOI=10.1016/0042-6822(84)90012-6;
RA   Eiserling F.A., Corso J., Feng S., Epstein R.H.;
RT   "Intracellular morphogenesis of bacteriophage T4. II. Head morphogenesis.";
RL   Virology 137:95-101(1984).
CC   -!- FUNCTION: [Capsid assembly scaffolding protein]: Scaffolding protein
CC       involved in the icosahedric procapsid assembly. Coassembles with the
CC       capsid proteins to form the procapsid, in which the scaffolding protein
CC       is found within the external shell of icosahedrally arranged capsid
CC       protein subunits. In a subsequent step the scaffolding protein
CC       molecules are cleaved by the viral protease and released, except for
CC       the internal peptide VII. {ECO:0000305|PubMed:6382791}.
CC   -!- FUNCTION: [Internal peptide VII]: Cleavage product of Gp22 that is
CC       incorporated into the mature phage head. {ECO:0000269|PubMed:6382791}.
CC   -!- SUBCELLULAR LOCATION: [Internal peptide VII]: Virion.
CC   -!- SIMILARITY: Belongs to the T4likevirus capsid assembly scaffolding
CC       protein family. {ECO:0000305}.
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DR   EMBL; X16492; CAA34509.1; -; Genomic_DNA.
DR   EMBL; K01765; AAA32502.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42427.1; -; Genomic_DNA.
DR   EMBL; D10978; BAA01752.1; -; Genomic_DNA.
DR   PIR; S06066; Z2BPT4.
DR   RefSeq; NP_049786.1; NC_000866.4.
DR   SMR; P04534; -.
DR   GeneID; 1258775; -.
DR   KEGG; vg:1258775; -.
DR   Proteomes; UP000009087; Genome.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Viral capsid assembly;
KW   Viral release from host cell; Virion.
FT   CHAIN           1..269
FT                   /note="Capsid assembly scaffolding protein"
FT                   /id="PRO_0000045381"
FT   PEPTIDE         62..84
FT                   /note="Internal peptide VII"
FT                   /id="PRO_0000003335"
FT   CONFLICT        89
FT                   /note="F -> S (in Ref. 3; AAA32502)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   269 AA;  29905 MW;  2374D3D8789CB9A2 CRC64;
     MLKEQLIAEA QKIDASVALD SIFESVNISP EAKETFGTVF EATVKQHAVK LAESHIAKIA
     EKAEEEVEKN KEEAEEKAEK KIAEQASKFI DHLAKEWLAE NKLAVDKGIK AELFESMLGG
     LKELFVEHNV VVPEESVDVV AEMEEELQEH KEESPRLFEE LNMRDAYINY VQREVALSES
     TKDLTESQKE KVSALVEGMD YSDAFSSKLS AIVEMVKKSN KDESTITESI NTPDTEAAGL
     NFVTEAVEDK AAQGAEDIVS VYAKVASRF