SCAM4_HUMAN
ID SCAM4_HUMAN Reviewed; 229 AA.
AC Q969E2; Q8N2N1; Q8NAV0;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Secretory carrier-associated membrane protein 4;
DE Short=Secretory carrier membrane protein 4;
GN Name=SCAMP4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-194, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Probably involved in membrane protein trafficking.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q969E2; Q9BV23: ABHD6; NbExp=3; IntAct=EBI-4403649, EBI-3916106;
CC Q969E2; Q13520: AQP6; NbExp=3; IntAct=EBI-4403649, EBI-13059134;
CC Q969E2; Q13323: BIK; NbExp=3; IntAct=EBI-4403649, EBI-700794;
CC Q969E2; Q99675: CGRRF1; NbExp=3; IntAct=EBI-4403649, EBI-2130213;
CC Q969E2; O95471: CLDN7; NbExp=3; IntAct=EBI-4403649, EBI-740744;
CC Q969E2; Q15125: EBP; NbExp=3; IntAct=EBI-4403649, EBI-3915253;
CC Q969E2; P34910-2: EVI2B; NbExp=3; IntAct=EBI-4403649, EBI-17640610;
CC Q969E2; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-4403649, EBI-13345167;
CC Q969E2; O15354: GPR37; NbExp=3; IntAct=EBI-4403649, EBI-15639515;
CC Q969E2; P28799: GRN; NbExp=3; IntAct=EBI-4403649, EBI-747754;
CC Q969E2; P28799-2: GRN; NbExp=3; IntAct=EBI-4403649, EBI-25860013;
CC Q969E2; P48051: KCNJ6; NbExp=3; IntAct=EBI-4403649, EBI-12017638;
CC Q969E2; Q99750: MDFI; NbExp=3; IntAct=EBI-4403649, EBI-724076;
CC Q969E2; Q14973: SLC10A1; NbExp=3; IntAct=EBI-4403649, EBI-3923031;
CC Q969E2; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-4403649, EBI-18159983;
CC Q969E2; Q9NPE6: SPAG4; NbExp=3; IntAct=EBI-4403649, EBI-10819434;
CC Q969E2; Q9BVK8: TMEM147; NbExp=3; IntAct=EBI-4403649, EBI-348587;
CC Q969E2; O76024: WFS1; NbExp=3; IntAct=EBI-4403649, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q969E2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q969E2-2; Sequence=VSP_026446;
CC Name=3;
CC IsoId=Q969E2-3; Sequence=VSP_026447;
CC -!- SIMILARITY: Belongs to the SCAMP family. {ECO:0000305}.
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DR EMBL; AK074586; BAC11075.1; -; mRNA.
DR EMBL; AK074967; BAC11322.1; -; mRNA.
DR EMBL; AK092056; BAC03797.1; -; mRNA.
DR EMBL; BC011747; AAH11747.1; -; mRNA.
DR EMBL; BC016509; AAH16509.1; -; mRNA.
DR EMBL; BC062598; AAH62598.1; -; mRNA.
DR CCDS; CCDS45903.1; -. [Q969E2-1]
DR CCDS; CCDS86689.1; -. [Q969E2-2]
DR RefSeq; NP_001316468.1; NM_001329539.1.
DR RefSeq; NP_001316469.1; NM_001329540.1. [Q969E2-2]
DR RefSeq; NP_524558.1; NM_079834.3. [Q969E2-1]
DR AlphaFoldDB; Q969E2; -.
DR BioGRID; 125230; 49.
DR IntAct; Q969E2; 30.
DR MINT; Q969E2; -.
DR STRING; 9606.ENSP00000316007; -.
DR TCDB; 8.A.103.1.4; the secretory carrier-associated membrane protein (scamp) family.
DR GlyGen; Q969E2; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q969E2; -.
DR PhosphoSitePlus; Q969E2; -.
DR SwissPalm; Q969E2; -.
DR BioMuta; SCAMP4; -.
DR DMDM; 47117277; -.
DR EPD; Q969E2; -.
DR jPOST; Q969E2; -.
DR MassIVE; Q969E2; -.
DR MaxQB; Q969E2; -.
DR PaxDb; Q969E2; -.
DR PeptideAtlas; Q969E2; -.
DR PRIDE; Q969E2; -.
DR ProteomicsDB; 75740; -. [Q969E2-1]
DR ProteomicsDB; 75741; -. [Q969E2-2]
DR ProteomicsDB; 75742; -. [Q969E2-3]
DR Antibodypedia; 22844; 93 antibodies from 23 providers.
DR DNASU; 113178; -.
DR Ensembl; ENST00000316097.13; ENSP00000316007.7; ENSG00000227500.10. [Q969E2-1]
DR Ensembl; ENST00000409472.5; ENSP00000386865.1; ENSG00000227500.10. [Q969E2-2]
DR Ensembl; ENST00000621748.1; ENSP00000479083.1; ENSG00000227500.10. [Q969E2-1]
DR GeneID; 113178; -.
DR KEGG; hsa:113178; -.
DR MANE-Select; ENST00000316097.13; ENSP00000316007.7; NM_079834.4; NP_524558.1.
DR UCSC; uc002luj.5; human. [Q969E2-1]
DR CTD; 113178; -.
DR DisGeNET; 113178; -.
DR GeneCards; SCAMP4; -.
DR HGNC; HGNC:30385; SCAMP4.
DR HPA; ENSG00000227500; Low tissue specificity.
DR MalaCards; SCAMP4; -.
DR MIM; 613764; gene.
DR neXtProt; NX_Q969E2; -.
DR OpenTargets; ENSG00000227500; -.
DR PharmGKB; PA134983341; -.
DR VEuPathDB; HostDB:ENSG00000227500; -.
DR eggNOG; KOG3088; Eukaryota.
DR GeneTree; ENSGT00940000162150; -.
DR HOGENOM; CLU_066546_1_0_1; -.
DR InParanoid; Q969E2; -.
DR OMA; MMAIAII; -.
DR PhylomeDB; Q969E2; -.
DR TreeFam; TF313797; -.
DR PathwayCommons; Q969E2; -.
DR SignaLink; Q969E2; -.
DR BioGRID-ORCS; 113178; 20 hits in 1069 CRISPR screens.
DR ChiTaRS; SCAMP4; human.
DR GenomeRNAi; 113178; -.
DR Pharos; Q969E2; Tdark.
DR PRO; PR:Q969E2; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q969E2; protein.
DR Bgee; ENSG00000227500; Expressed in right uterine tube and 139 other tissues.
DR ExpressionAtlas; Q969E2; baseline and differential.
DR Genevisible; Q969E2; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0055038; C:recycling endosome membrane; IBA:GO_Central.
DR GO; GO:0032588; C:trans-Golgi network membrane; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR InterPro; IPR007273; SCAMP.
DR PANTHER; PTHR10687; PTHR10687; 1.
DR Pfam; PF04144; SCAMP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..229
FT /note="Secretory carrier-associated membrane protein 4"
FT /id="PRO_0000191259"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 208..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 194
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 99..132
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_026446"
FT VAR_SEQ 132..229
FT /note="CGWLSAIGFFQYSPGAAVVMLLPAIMFSVSAAMMAIAIMKVHRIYRGAGGSF
FT QKAQTEWNTGTWRNPPSREAQYNNFSGNSLPEYPTVPSYPGSGQWP -> G (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_026447"
FT VARIANT 49
FT /note="A -> T (in dbSNP:rs45562539)"
FT /id="VAR_061783"
SQ SEQUENCE 229 AA; 25728 MW; FA3EB084B3970334 CRC64;
MSEKENNFPP LPKFIPVKPC FYQNFSDEIP VEHQVLVKRI YRLWMFYCAT LGVNLIACLA
WWIGGGSGTN FGLAFVWLLL FTPCGYVCWF RPVYKAFRAD SSFNFMAFFF IFGAQFVLTV
IQAIGFSGWG ACGWLSAIGF FQYSPGAAVV MLLPAIMFSV SAAMMAIAIM KVHRIYRGAG
GSFQKAQTEW NTGTWRNPPS REAQYNNFSG NSLPEYPTVP SYPGSGQWP
