ID   SCAM5_DANRE             Reviewed;         230 AA.
AC   Q6P0C7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Secretory carrier-associated membrane protein 5;
DE            Short=Secretory carrier membrane protein 5;
GN   Name=scamp5;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the calcium-dependent exocytosis of signal
CC       sequence-containing cytokines. Probably acts in cooperation with the
CC       SNARE machinery (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Multi-
CC       pass membrane protein {ECO:0000250}. Golgi apparatus, trans-Golgi
CC       network membrane {ECO:0000250}; Multi-pass membrane protein
CC       {ECO:0000250}. Recycling endosome membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle,
CC       synaptic vesicle membrane {ECO:0000250}; Multi-pass membrane protein
CC       {ECO:0000250}. Note=Mainly localizes in Golgi apparatus membrane. Upon
CC       calcium-triggered exocytosis, it translocates to the cell membrane.
CC       Highly enriched in synaptic vesicles (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SCAMP family. SCAMP5 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC065670; AAH65670.1; -; mRNA.
DR   RefSeq; NP_991110.1; NM_205547.1.
DR   AlphaFoldDB; Q6P0C7; -.
DR   SMR; Q6P0C7; -.
DR   STRING; 7955.ENSDARP00000031172; -.
DR   PaxDb; Q6P0C7; -.
DR   Ensembl; ENSDART00000028338; ENSDARP00000031172; ENSDARG00000018743.
DR   GeneID; 324467; -.
DR   KEGG; dre:324467; -.
DR   CTD; 324467; -.
DR   ZFIN; ZDB-GENE-030131-3188; scamp5a.
DR   eggNOG; KOG3088; Eukaryota.
DR   GeneTree; ENSGT00940000157577; -.
DR   HOGENOM; CLU_066546_1_0_1; -.
DR   InParanoid; Q6P0C7; -.
DR   OMA; KAQQEWT; -.
DR   OrthoDB; 995882at2759; -.
DR   PhylomeDB; Q6P0C7; -.
DR   TreeFam; TF313797; -.
DR   PRO; PR:Q6P0C7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 25.
DR   Bgee; ENSDARG00000018743; Expressed in brain and 21 other tissues.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0055038; C:recycling endosome membrane; IBA:GO_Central.
DR   GO; GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0032588; C:trans-Golgi network membrane; IBA:GO_Central.
DR   GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR   GO; GO:0045956; P:positive regulation of calcium ion-dependent exocytosis; ISS:UniProtKB.
DR   GO; GO:0001819; P:positive regulation of cytokine production; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR   InterPro; IPR007273; SCAMP.
DR   PANTHER; PTHR10687; PTHR10687; 1.
DR   Pfam; PF04144; SCAMP; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasmic vesicle; Endosome; Exocytosis; Golgi apparatus;
KW   Membrane; Protein transport; Reference proteome; Synapse; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..230
FT                   /note="Secretory carrier-associated membrane protein 5"
FT                   /id="PRO_0000370554"
FT   TOPO_DOM        1..36
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        58..64
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        86..99
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        123..145
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        167..230
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   230 AA;  25568 MW;  C8080603A099B786 CRC64;
     MAENNFPPLP RFIPLKPCFY QDFNEIPDQH RTMCKRLYYL WILNSATLAV NLIGCLAWMC
     GGGGATNFGM AILWLILFTP CSYVCWFRPI YKAFKSDSSF NFMAFFFVFM AQVVISIIQT
     VGIPGWGVCG WLATITFFST NIGSAVVMLI PTIMFTAVAV LSFIALTKVH NFYRGSGGSM
     SKAQEEWTSG AWKNPHVQQA AQQAAMGAAQ GAMQGQQYSA APTYNYDDPM