SCARL_ARATH
ID SCARL_ARATH Reviewed; 2028 AA.
AC Q5XPK0; O49528;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Scar-like domain-containing protein WAVE 5;
GN Name=WAVE5; OrderedLocusNames=At4g18600; ORFNames=F28J12.260;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=15534215; DOI=10.1073/pnas.0407392101;
RA Frank M., Egile C., Dyachok J., Djakovic S., Nolasco M., Li R., Smith L.G.;
RT "Activation of Arp2/3 complex-dependent actin polymerization by plant
RT proteins distantly related to Scar/WAVE.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16379-16384(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP INTERACTION WITH ABI1 AND ABI2.
RX PubMed=17267444; DOI=10.1242/dev.02792;
RA Uhrig J.F., Mutondo M., Zimmermann I., Deeks M.J., Machesky L.M.,
RA Thomas P., Uhrig S., Rambke C., Hussey P.J., Huelskamp M.;
RT "The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell
RT morphogenesis.";
RL Development 134:967-977(2007).
CC -!- SUBUNIT: Interacts with ABI1 and ABI2. {ECO:0000269|PubMed:17267444}.
CC -!- TISSUE SPECIFICITY: Expressed in expanding cotyledons, expanding leaves
CC and expanding siliques containing developing embryos. Detected in
CC unopened flower buds. Reduced expression in mature leaves.
CC {ECO:0000269|PubMed:15534215}.
CC -!- DOMAIN: Contains the N-terminal Scar homology domain (SHD), but unlike
CC the genuine SCAR proteins, lacks the C-terminal VCA (verprolin
CC homology/cofilin homology/acidic) domain.
CC -!- SIMILARITY: Belongs to the SCAR/WAVE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16740.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78862.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY743923; AAU93848.1; -; mRNA.
DR EMBL; AL021710; CAA16740.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161549; CAB78862.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84067.1; -; Genomic_DNA.
DR PIR; T04556; T04556.
DR RefSeq; NP_193595.3; NM_117976.4.
DR AlphaFoldDB; Q5XPK0; -.
DR SMR; Q5XPK0; -.
DR BioGRID; 12887; 3.
DR IntAct; Q5XPK0; 3.
DR STRING; 3702.AT4G18600.1; -.
DR iPTMnet; Q5XPK0; -.
DR PaxDb; Q5XPK0; -.
DR PRIDE; Q5XPK0; -.
DR ProteomicsDB; 232870; -.
DR EnsemblPlants; AT4G18600.1; AT4G18600.1; AT4G18600.
DR GeneID; 827594; -.
DR Gramene; AT4G18600.1; AT4G18600.1; AT4G18600.
DR KEGG; ath:AT4G18600; -.
DR Araport; AT4G18600; -.
DR TAIR; locus:2124598; AT4G18600.
DR eggNOG; ENOG502QSPV; Eukaryota.
DR HOGENOM; CLU_230242_0_0_1; -.
DR InParanoid; Q5XPK0; -.
DR OrthoDB; 594491at2759; -.
DR PRO; PR:Q5XPK0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q5XPK0; baseline and differential.
DR Genevisible; Q5XPK0; AT.
DR GO; GO:0005856; C:cytoskeleton; IEA:InterPro.
DR GO; GO:0071933; F:Arp2/3 complex binding; IBA:GO_Central.
DR GO; GO:0034237; F:protein kinase A regulatory subunit binding; IBA:GO_Central.
DR GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:2000601; P:positive regulation of Arp2/3 complex-mediated actin nucleation; IBA:GO_Central.
DR InterPro; IPR028288; SCAR/WAVE_fam.
DR PANTHER; PTHR12902; PTHR12902; 6.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..2028
FT /note="Scar-like domain-containing protein WAVE 5"
FT /id="PRO_0000189008"
FT REGION 171..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 258..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..443
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 877..903
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1469..1491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1511..1536
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1639..1663
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1710..1754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1911..1931
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1977..2028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1472..1491
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1511..1525
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1980..2007
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 140
FT /note="R -> G (in Ref. 1; AAU93848)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="K -> R (in Ref. 1; AAU93848)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="L -> S (in Ref. 1; AAU93848)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="E -> G (in Ref. 1; AAU93848)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2028 AA; 222122 MW; 3353E9158616F0E2 CRC64;
MPLVRFKIRN ELSLGGPEIQ RSASVEDEEP KAILGAVEVA GLIGILRQLG DLAEFSAEVF
NGLQEEVTVT ASRCQKLTSR VRRIESALSP LEKAVLSQTS HIHFAYTAGS EWHPRIRNGH
SHFVQSDLPL CVMESYEQCR DPPPLHLLDR FAVGGPGSCL RKYSDPTFFR KELSNPSKTD
DIKVQRDQAH RKRKKKRLPQ RNICRSNAVS TSDETNGAHL SSFTDDRPTT SRSTSTVDMP
RSSNMQDLSD IVDQSYLQGQ SGAQEQSEAQ VQSDFQESSK ARDSITGSGY IEYVINQSPV
DKPEVKLVEG FLSGSLCPAD RIGSTVPEGC IEVVDDNILY SPSEDLLVPS ASNVCDEKKE
TLESMVEKSR KDDEPSELHE SKFGPVTPDR VRQNQRDFDR TYILFDEVDI VGEKQSKSQA
NNIDGTLGIE NEGEDKSEQE SEADEFVDAR NTIESESESD IDGVPKPKLE HYFGDISTYC
SEDANSDNND GSEDITYEEM AHDPRHENSE DESCSGSYLP EDSNVSSCLS DPVCEETLFH
DENSQKPWEF FTMCPSLLAE KAVPDVTILR EEPVMAHPLF AGDSANEKIS SEERIISCPS
LKDAIPAEKI LPEEHLVNYP SLEAIPHEKI LPGESIAKYP SFAEIIPQEK ILSEKSLEEA
VLDNMTPAGE PDAAHPSFPK AVQDKKISPE VLDSKIFSVP KAVSQEPISL EEFVRIHPCL
AEAVPDERFL IEEPPSTCLS LTKAMPTEIL LPEKTLESSH YLEELPEEDI LQEKSVDSTH
PSCAKAAAET NLSPEVLDST KLSVAEAVPQ EQVSLEEFVG INPCLVEAVP DERLLPEEPD
TTYLSLTKAV AIEKVLSEEL LETYPSLAEL PEEEFLQEET DDATHTSEAV SDEEISQEVS
DSTNLSLEEA LPLEHISHEE FVGIDQCLVE VAPEERFLPE EPVITCLSLT MEGVLSEKSP
ETYPSLSELP EEKILDEEAD DAMHPCLSEA VSDEQSSPNV LGSTNSPVAE AVPQAQISVE
EFVGIDPCLV EAVPDMRDLP EEHITSCIYL TNVVPIEEVL PEEPFEACAS LGKLPKDKIS
QEESDEATHT LSCAKAESDE HVSLEVLNST NLSAAEAIPN EQVTLDEFVG IDPCLGEVVL
DEEVLPEQLD TTCRSLTKAA PIMTIFPEEP PEVYPSLEEL PEEKIAQEEE VDDATHPYFS
EAVCDEKIPP LEDPGSTHPS LEESVPYEEA SHEELVGTNP FLESAFPNEI GFPDEPGVTY
RSSAEAVSKE KNLPEESLPT YPSWAEVVPD EKISREELDS TYPSSAEAVF DENISGSEAP
GSTTETGRQN KTFPEKTFAT ENSLNEAVFD EKIPGSEAST STTETGPHNK TFPEEPFAME
NFLNEAVFDE KIPGSEAPVS TTETGLHNET FTEEPVATDI SLNEAVFGEI IPGSEAPGSA
TETGLHNKTF PEKPFATDLS LKEAVFDEKI PGSEASSSTT ETSPHNKTFP RETITTDLSS
TEAVFDEKIT GSGVPSFTTE TGSHNKCFPE EPVPKENILP KEPAAAYLAL AEGIPDQKVF
LDDAALLLFA EAIFDQKFSP EVPDSTYPSL KEPEMHVAAP CVVTDLPAKN IKVKEGEVHN
EPYTASDVSM NQKSGLLEPE STERTFPSSG GTVTISPDTQ NSLPNGTSVE SISIWSNGGL
LGLAPLKPPV FAEPNSGSQH IKHEINEASV LSTRKQESSS RSVENAEKSS LPLIVSDPTS
QQQSNMSSLS PMQSTGTSFR VFGLSHRLLM AGFRGNSSST CKFESVPSSS YDTRVAAIED
RTQQSPGGSS FEEQLDYESS LFGSPTSSPP VEHMKISFNP IEASPVPKLK LRIPCQPRYN
GENADMFPSF QLVPEASNSD DGDDNSDTFC QSSPCVSDYC LSDSELWESD ESPRISVSSL
KQVEERSRHG DMGSFSGSFL DLPCYDAVDH QSTFSRLEQE QVPEYKPSVS EIIRAWPPNQ
PKSSPCNEAN VDANTVSKKT QDQSLGLVAT DDEGGDSVCL DEHKTKGI
