SCA_DROME
ID SCA_DROME Reviewed; 799 AA.
AC P21520; Q0E998; Q8MQI8; Q9V6G9;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2003, sequence version 3.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Protein scabrous;
DE Flags: Precursor;
GN Name=sca; ORFNames=CG17579;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=2175046; DOI=10.1126/science.2175046;
RA Baker N.E., Mlodzik M., Rubin G.M.;
RT "Spacing differentiation in the developing Drosophila eye: a fibrinogen-
RT related lateral inhibitor encoded by scabrous.";
RL Science 250:1370-1377(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12756167; DOI=10.1242/dev.00495;
RA Li Y., Fetchko M., Lai Z.C., Baker N.E.;
RT "Scabrous and Gp150 are endosomal proteins that regulate Notch activity.";
RL Development 130:2819-2827(2003).
CC -!- FUNCTION: Involved in regulation of neurogenesis. May encode a lateral
CC inhibitor of R8 differentiation. In conjunction with Gp150, promotes
CC Notch activation in response to Delta by regulating acquisition of
CC insensitivity to Delta in a subset of cells.
CC {ECO:0000269|PubMed:12756167, ECO:0000269|PubMed:2175046}.
CC -!- SUBCELLULAR LOCATION: Late endosome {ECO:0000269|PubMed:12756167,
CC ECO:0000269|PubMed:2175046}. Note=Colocalizes in late endosomes with
CC Gp150.
CC -!- PTM: Possesses five pairs of dibasic residues that may be the target of
CC proteolytic processing.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28880.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M60065; AAA28880.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE013599; AAF58455.2; -; Genomic_DNA.
DR EMBL; AY129456; AAM76198.1; -; mRNA.
DR PIR; A39832; A39832.
DR RefSeq; NP_476710.2; NM_057362.3.
DR RefSeq; NP_725230.2; NM_165951.2.
DR AlphaFoldDB; P21520; -.
DR SMR; P21520; -.
DR BioGRID; 62183; 14.
DR IntAct; P21520; 2.
DR MINT; P21520; -.
DR STRING; 7227.FBpp0086969; -.
DR GlyGen; P21520; 6 sites.
DR PaxDb; P21520; -.
DR DNASU; 36411; -.
DR EnsemblMetazoa; FBtr0087855; FBpp0086968; FBgn0003326.
DR EnsemblMetazoa; FBtr0087856; FBpp0086969; FBgn0003326.
DR GeneID; 36411; -.
DR KEGG; dme:Dmel_CG17579; -.
DR CTD; 36411; -.
DR FlyBase; FBgn0003326; sca.
DR VEuPathDB; VectorBase:FBgn0003326; -.
DR eggNOG; KOG2579; Eukaryota.
DR HOGENOM; CLU_021665_0_0_1; -.
DR InParanoid; P21520; -.
DR OMA; DADMRTE; -.
DR OrthoDB; 497548at2759; -.
DR PhylomeDB; P21520; -.
DR Reactome; R-DME-114608; Platelet degranulation.
DR Reactome; R-DME-1474228; Degradation of the extracellular matrix.
DR Reactome; R-DME-1474244; Extracellular matrix organization.
DR Reactome; R-DME-1566977; Fibronectin matrix formation.
DR Reactome; R-DME-202733; Cell surface interactions at the vascular wall.
DR Reactome; R-DME-210993; Tie2 Signaling.
DR Reactome; R-DME-216083; Integrin cell surface interactions.
DR Reactome; R-DME-3000170; Syndecan interactions.
DR Reactome; R-DME-3000178; ECM proteoglycans.
DR Reactome; R-DME-354192; Integrin signaling.
DR Reactome; R-DME-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-DME-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DME-5674135; MAP2K and MAPK activation.
DR Reactome; R-DME-8957275; Post-translational protein phosphorylation.
DR Reactome; R-DME-9634597; GPER1 signaling.
DR SignaLink; P21520; -.
DR BioGRID-ORCS; 36411; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 36411; -.
DR PRO; PR:P21520; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0003326; Expressed in eye disc (Drosophila) and 24 other tissues.
DR Genevisible; P21520; DM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:FlyBase.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0008587; P:imaginal disc-derived wing margin morphogenesis; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IGI:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0016318; P:ommatidial rotation; IMP:FlyBase.
DR GO; GO:0097305; P:response to alcohol; IMP:FlyBase.
DR CDD; cd00087; FReD; 1.
DR Gene3D; 3.90.215.10; -; 1.
DR InterPro; IPR036056; Fibrinogen-like_C.
DR InterPro; IPR014716; Fibrinogen_a/b/g_C_1.
DR InterPro; IPR002181; Fibrinogen_a/b/g_C_dom.
DR InterPro; IPR020837; Fibrinogen_CS.
DR Pfam; PF00147; Fibrinogen_C; 1.
DR SMART; SM00186; FBG; 1.
DR SUPFAM; SSF56496; SSF56496; 1.
DR PROSITE; PS00514; FIBRINOGEN_C_1; 1.
DR PROSITE; PS51406; FIBRINOGEN_C_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Disulfide bond; Endosome;
KW Glycoprotein; Neurogenesis; Notch signaling pathway; Reference proteome;
KW Signal.
FT SIGNAL 1..51
FT /evidence="ECO:0000255"
FT CHAIN 52..799
FT /note="Protein scabrous"
FT /id="PRO_0000009108"
FT DOMAIN 533..737
FT /note="Fibrinogen C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00739"
FT REGION 287..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 489..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 287..312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 587
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 618
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 660
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 744
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 787
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 542..568
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00739"
FT DISULFID 687..700
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00739"
FT CONFLICT 301
FT /note="G -> C (in Ref. 1; AAA28880)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 799 AA; 90119 MW; 87E1B4D60D4F5C29 CRC64;
MRDWQTFPDL QKKKVSRDHL NCPATMAGSN VLWPILLAVV LLQISVAFVS GAASGGVVLS
DVNNMLRDAK VVTSEKPVVH SKQETEAPES SVELLRFVDD DEDSEDISSI ERQDGRTMES
KKMADQVRLL TKQLNALMLR RREDYEMLEH NLRKSLRLTT NANSVDADMR SELNQLREEL
AALRSSQSGN KERLTVEWLQ QTISEIRKQL VDLQRTASNV AQDVQQRSST FEDLATIRSD
YQQLKLDLAA QRERQQQTEV YVQELREEML QQEQDFQHAL VKLQQRTRKD GSSASVEEES
GSQEANQEQT GLETTADHKR RHCRFQSEQI HQLQLAQRNL RRQVNGLRFH HIDERVRSIE
VEQHRIANAN FNLSSQIASL DKLHTSMLEL LEDVEGLQTK MDKSIPELRH EISKLEFANA
QITSEQSLIR EEGTNAARSL QAMAVSVSVL QEEREGMRKL SANVDQLRTN VDRLQSLVND
EMKNKLTHLN KPHKRPHHQN VQAQMPQDDS PIDSVLAETL VSELENVETQ YEAIINKLPH
DCSEVHTQTD GLHLIAPAGQ RHPLMTHCTA DGWTTVQRRF DGSADFNRSW ADYAQGFGAP
GGEFWIGNEQ LHHLTLDNCS RLQVQMQDIY DNVWVAEYKR FYISSRADGY RLHIAEYSGN
ASDALNYQQG MQFSAIDDDR DISQTHCAAN YEGGWWFSHC QHANLNGRYN LGLTWFDAAR
NEWIAVKSSR MLVKRLPAVE CQANASASGA FVSVSGSAAD AAPSSGATTT TTTATAAPAT
VTTPKTNNSV VQFVAAGQA
