SCC2_ARATH
ID SCC2_ARATH Reviewed; 1846 AA.
AC A5HEI1; A7UDC4; F4K9U3; Q9LF28;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Sister chromatid cohesion protein SCC2 {ECO:0000303|PubMed:19228337};
DE AltName: Full=Protein EMBRYO DEFECTIVE 2773 {ECO:0000303|PubMed:15266054};
DE AltName: Full=Protein SISTER-CHROMATID COHESION 2 {ECO:0000303|PubMed:19228337};
DE Short=AtSCC2 {ECO:0000303|PubMed:19228337};
GN Name=SCC2 {ECO:0000303|PubMed:19228337};
GN Synonyms=EMB2773 {ECO:0000303|PubMed:15266054};
GN OrderedLocusNames=At5g15540 {ECO:0000312|Araport:AT5G15540};
GN ORFNames=T20K14.150 {ECO:0000312|EMBL:CAC01753.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), FUNCTION, DISRUPTION
RP PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=19228337; DOI=10.1111/j.1365-313x.2009.03845.x;
RA Sebastian J., Ravi M., Andreuzza S., Panoli A.P., Marimuthu M.P.A.,
RA Siddiqi I.;
RT "The plant adherin AtSCC2 is required for embryogenesis and sister-
RT chromatid cohesion during meiosis in Arabidopsis.";
RL Plant J. 59:1-13(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION [LARGE SCALE ANALYSIS], AND DISRUPTION PHENOTYPE [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=19533160; DOI=10.1007/s00412-009-0220-x;
RA Schubert V., Weissleder A., Ali H., Fuchs J., Lermontova I., Meister A.,
RA Schubert I.;
RT "Cohesin gene defects may impair sister chromatid alignment and genome
RT stability in Arabidopsis thaliana.";
RL Chromosoma 118:591-605(2009).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH SCC4.
RC STRAIN=cv. Columbia;
RX PubMed=28137757; DOI=10.1242/jcs.196865;
RA Minina E.A., Reza S.H., Gutierrez-Beltran E., Elander P.H., Bozhkov P.V.,
RA Moschou P.N.;
RT "The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis.";
RL J. Cell Sci. 130:1051-1063(2017).
CC -!- FUNCTION: Essential protein required for cell fate determination during
CC embryogenesis (PubMed:19228337, PubMed:15266054, PubMed:28137757).
CC Involved in sister chromatid cohesion during meiosis and mitosis
CC (PubMed:19228337, PubMed:19533160). Forms a complex with SCC4, which is
CC required for the association of the cohesin complex with chromosomes
CC (PubMed:28137757). Plays a structural role in chromatin, especially in
CC centromere organization, chromosomal axis formation, and distribution
CC of the cohesin subunit SCC3 on chromosomes (PubMed:19228337).
CC {ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:19228337,
CC ECO:0000269|PubMed:19533160, ECO:0000269|PubMed:28137757}.
CC -!- SUBUNIT: Interacts with SCC4 to form the cohesin loading complex.
CC {ECO:0000269|PubMed:28137757}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q04002}.
CC Chromosome, centromere {ECO:0000250|UniProtKB:Q04002}. Note=Associates
CC with chromatin. {ECO:0000250|UniProtKB:Q04002}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A5HEI1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A5HEI1-2; Sequence=VSP_058980;
CC Name=3;
CC IsoId=A5HEI1-3; Sequence=VSP_058981;
CC -!- TISSUE SPECIFICITY: Expressed in leaves, inflorescence and siliques.
CC {ECO:0000269|PubMed:19228337}.
CC -!- DEVELOPMENTAL STAGE: Detected throughout the embryo, covering all
CC stages of development from pre-globular to torpedo stages. Also
CC detected in the suspensor and endosperm. {ECO:0000269|PubMed:19228337}.
CC -!- DISRUPTION PHENOTYPE: Defective embryo arrested at preglobular/early
CC globular stage with the formation of giant endosperm nuclei
CC (PubMed:19228337, PubMed:15266054, PubMed:28137757). Suspensor
CC overproliferation phenotype preceded by ectopic auxin maxima
CC distribution (PubMed:28137757). Reduced sister chromatid cohesion
CC (PubMed:19533160). In conditional RNAi plants, sterility, arising from
CC several defects in meiotic chromosome organization (e.g. failure of
CC homologous pairing, loss of sister-chromatid cohesion, mixed
CC segregation of chromosomes and chromosome fragmentation) and leading to
CC shrunken and inviable pollen grains, and degeneration of the embryo
CC sac. In the meiocytes, aberrant distribution of the cohesin subunit
CC SCC3 on chromosomes, and defects in chromosomal axis formation
CC (PubMed:19228337). {ECO:0000269|PubMed:15266054,
CC ECO:0000269|PubMed:19228337, ECO:0000269|PubMed:19533160,
CC ECO:0000269|PubMed:28137757}.
CC -!- SIMILARITY: Belongs to the SCC2/Nipped-B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC01753.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Seed defective Arabidopsis mutants;
CC URL="http://seedgenes.org/MutantList";
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DR EMBL; EF520004; ABQ12620.1; -; mRNA.
DR EMBL; EU046617; ABS88747.1; -; mRNA.
DR EMBL; AL391143; CAC01753.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92174.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92175.1; -; Genomic_DNA.
DR PIR; T51532; T51532.
DR RefSeq; NP_001190317.1; NM_001203388.1. [A5HEI1-2]
DR RefSeq; NP_197058.2; NM_121558.4. [A5HEI1-1]
DR AlphaFoldDB; A5HEI1; -.
DR SMR; A5HEI1; -.
DR STRING; 3702.AT5G15540.1; -.
DR iPTMnet; A5HEI1; -.
DR PaxDb; A5HEI1; -.
DR PRIDE; A5HEI1; -.
DR ProteomicsDB; 226672; -. [A5HEI1-1]
DR EnsemblPlants; AT5G15540.1; AT5G15540.1; AT5G15540. [A5HEI1-1]
DR EnsemblPlants; AT5G15540.2; AT5G15540.2; AT5G15540. [A5HEI1-2]
DR GeneID; 831407; -.
DR Gramene; AT5G15540.1; AT5G15540.1; AT5G15540. [A5HEI1-1]
DR Gramene; AT5G15540.2; AT5G15540.2; AT5G15540. [A5HEI1-2]
DR KEGG; ath:AT5G15540; -.
DR Araport; AT5G15540; -.
DR TAIR; locus:2180942; AT5G15540.
DR eggNOG; KOG1020; Eukaryota.
DR HOGENOM; CLU_000763_2_1_1; -.
DR InParanoid; A5HEI1; -.
DR OMA; VVVIYRC; -.
DR OrthoDB; 608077at2759; -.
DR PhylomeDB; A5HEI1; -.
DR PRO; PR:A5HEI1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; A5HEI1; baseline and differential.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0090694; C:Scc2-Scc4 cohesin loading complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0034508; P:centromere complex assembly; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0034087; P:establishment of mitotic sister chromatid cohesion; IBA:GO_Central.
DR GO; GO:0071169; P:establishment of protein localization to chromatin; IBA:GO_Central.
DR GO; GO:0051177; P:meiotic sister chromatid cohesion; IMP:TAIR.
DR GO; GO:0061780; P:mitotic cohesin loading; IEA:InterPro.
DR GO; GO:0010468; P:regulation of gene expression; IEA:InterPro.
DR GO; GO:1990414; P:replication-born double-strand break repair via sister chromatid exchange; IBA:GO_Central.
DR GO; GO:0007062; P:sister chromatid cohesion; IMP:TAIR.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR026003; Cohesin_HEAT.
DR InterPro; IPR024986; Nipped-B_C.
DR InterPro; IPR033031; Scc2/Nipped-B.
DR InterPro; IPR019786; Zinc_finger_PHD-type_CS.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR001965; Znf_PHD.
DR InterPro; IPR019787; Znf_PHD-finger.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR21704; PTHR21704; 1.
DR Pfam; PF12765; Cohesin_HEAT; 1.
DR Pfam; PF12830; Nipped-B_C; 1.
DR SMART; SM00249; PHD; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS01359; ZF_PHD_1; 1.
DR PROSITE; PS50016; ZF_PHD_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Meiosis; Metal-binding; Mitosis; Nucleus;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..1846
FT /note="Sister chromatid cohesion protein SCC2"
FT /id="PRO_0000440650"
FT REPEAT 243..282
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 298..335
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 438..460
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 461..499
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 532..572
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 613..631
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 632..672
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 781..818
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 869..906
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 945..982
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 984..1021
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 1053..1097
FT /note="HEAT 12"
FT /evidence="ECO:0000255"
FT REPEAT 1113..1152
FT /note="HEAT 13"
FT /evidence="ECO:0000255"
FT REPEAT 1174..1213
FT /note="HEAT 14"
FT /evidence="ECO:0000255"
FT REPEAT 1227..1267
FT /note="HEAT 15"
FT /evidence="ECO:0000255"
FT REPEAT 1277..1314
FT /note="HEAT 16"
FT /evidence="ECO:0000255"
FT REPEAT 1427..1464
FT /note="HEAT 17"
FT /evidence="ECO:0000255"
FT REPEAT 1475..1512
FT /note="HEAT 18"
FT /evidence="ECO:0000255"
FT REPEAT 1533..1575
FT /note="HEAT 19"
FT /evidence="ECO:0000255"
FT ZN_FING 699..748
FT /note="PHD-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00146"
FT REGION 175..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1790..1846
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..212
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 122..126
FT /note="EYVTP -> DLS (in isoform 2)"
FT /id="VSP_058980"
FT VAR_SEQ 393..1692
FT /note="Missing (in isoform 3)"
FT /id="VSP_058981"
SQ SEQUENCE 1846 AA; 206759 MW; E810C627DB8776A7 CRC64;
MSNPSSSGLG SSSGLTHFGI GLANTVQSEV TPYLPLPSLP IFCGAAEPGE FKLFDEVGQG
SGYRSLDRSE ILAQSSRIAN MLHETDVSYL DLRNEARAPD CNSGEHFQLY DLVLRCNPGA
FEYVTPGPTC DPLFTNEGPQ KIISEPSVPV KMQRQTDTHL ARSIEPEPVK RVLRPNHVED
HSWQHETLTN QSPKDVTAYD SRPETITMNE LSASKKPKGK KKRKDDLSSV QPDPSVLQES
IVQNFCEMLE DFCGRAEVPG DDRDEAEWSS VPVDEVRVLI NELMTIRSKM LLHMVPVDIL
SRLLRTLDHQ IHRAEGLSIY SEHSDSDSVL LVLGALESIH ASLAVMANSD MPKQLYKEEI
IERILEFSRH QMMAVMSAYD PSYRTGSKPA ENLAFEGDDD DDNPDHDMGS ASKRRRIVKN
SKVKKASVNR ISGAVNTALQ KLCTILGLLK DLLLVERLSD SCILQLLKTS ITTFLVENIQ
ILQLKAISLI GGIYNSYSQH RTYVIDEISQ LLWKLPSSKR ALRAYLLPDE EQRQIQMVTA
LLIQLVHNST SLPETSRQAA SGNSILETSV DVGYLTKCHE AATETCCLFW TRVLERFTSF
KGQDASEIKL IIENLVMDLL TALNLPEYPS VSPILEVLCV ILLHNAGLKS KDVSARIMAI
ELLGTIAARL KRDAVLCSKD RFWTLLESDS EISVDQVCTK DCTFCLGKRA GNLLVCQICQ
RRFHGDCLGL KELDISSRNW HCPLCVCKRQ LLVLQSYCKT DTKGTGKLES EESIENPSMI
TKTEVVQQML LNYLQDVGSA DDVHTFICWF YLCLWYKDVP KSQNKFKYYI ARLKAKSIIR
NSGATTSFLT RDAIKQITLA LGMNSSFSRG FDKILNMLLA SLRENAPNIR AKALRAVSII
VEADPEVLCD KRVQLAVEGR FCDSAISVRE AALELVGRHI ASHPDVGIKY FEKVAERIKD
TGVSVRKRAI KIIRDMCTSN PNFSEFTSAC AEILSRISDD ESSVQDLVCK TFYEFWFEEP
PGHHTQFASD ASSIPLELEK KTKQMVGLLS RTPNQQLLVT IIKRALALDF FPQAAKAAGI
NPVALASVRR RCELMCKCLL EKILQVEEMS REEGEVQVLP YVLVLHAFCL VDPGLCTPAS
DPTKFVITLQ PYLKSQADSR TGAQLLESII FIIDSVLPLI RKLPLSVTED LEQDLKHMIV
RHSFLTVVHA CVRCLCSVSK LAGKGVSIVE HLLQFFFKRL EAQGSDNTQI AGRSLFCLGL
LIRHGNSLIS TSGGKNFNLS GCLNLFKRHL RTEDIALKVR SLQALGFILI ARPEYMLEED
IGKIIETTLA DEANGRMKMQ ALQNMYEYLL DAEKQLGSEK ASDNTVNSVE QGGHNVPVAA
GAGDTNICGG IVQLFWDKIL GRCLDFDDQI RQTSLKIVEV VLRQGLVHPI TCVPYLIALE
TDPQEANQKL AHHLLMNMHE KYPAFFESRL GDGLQMSFIF MQSISQVTSE PNQSLQQKGS
TNMLGKNDHA SSTLTQARLG VSRIYKLIRG NRVSRNKFMT SIVRKFDNPT WNGSVISFLK
YCTETLALLP FTSPDEPLYL VYSINRVMQI RAGAVESNLK ALLHKDSAKT QHGNGAYQQD
PIPGHMNMMD LNTRIQEEPR HWNSYGHATL IDLNGSVYQD SRDQFTSYQV HNGKADVHKM
TSSDPPELST DDLQKIQVDC LAAIAIQLLL KLKRYLKVTY SLNDDRCQAY SPTEPLKPGD
PLSRQSVAFD LSETRTDLPS TYQDLVQRYQ EFKNAMREDT VDFTIYSTNV KRKRPTPRKT
SRSAKKTVAY NEDDDDDDND DRGWHGGGGR GAARRLNYST RSSNRR
