SCD2_ARATH
ID SCD2_ARATH Reviewed; 577 AA.
AC Q8RWD5; Q9M2Z7;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Coiled-coil domain-containing protein SCD2 {ECO:0000305};
DE AltName: Full=Protein STOMATAL CYTOKINESIS DEFECTIVE 2 {ECO:0000303|PubMed:24179130};
GN Name=SCD2 {ECO:0000303|PubMed:24179130};
GN OrderedLocusNames=At3g48860 {ECO:0000312|Araport:AT3G48860};
GN ORFNames=T21J18.130 {ECO:0000312|EMBL:CAB87914.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=24179130; DOI=10.1105/tpc.113.115162;
RA McMichael C.M., Reynolds G.D., Koch L.M., Wang C., Jiang N., Nadeau J.,
RA Sack F.D., Gelderman M.B., Pan J., Bednarek S.Y.;
RT "Mediation of clathrin-dependent trafficking during cytokinesis and cell
RT expansion by Arabidopsis stomatal cytokinesis defective proteins.";
RL Plant Cell 25:3910-3925(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Involved in growth and development through its role in
CC cytokinesis and polarized cell expansion. Required for plasma membrane
CC internalization. May function in clathrin-mediated membrane
CC trafficking, including plasma membrane endocytosis, essential to both
CC cytokinesis and cell expansion. {ECO:0000269|PubMed:24179130}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24179130};
CC Peripheral membrane protein {ECO:0000269|PubMed:24179130}. Cytoplasmic
CC vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:24179130}.
CC Note=Colocalized with clathrin at the plasma membrane.
CC {ECO:0000269|PubMed:24179130}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q8RWD5-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in the mature region of the primary root,
CC tips of short, young lateral roots, guard cells in the epidermis of
CC cotyledons and rosette leaves, the socket cells surrounding the base of
CC trichomes and hydathodes of fully expanded rosette leaves.
CC {ECO:0000269|PubMed:24179130}.
CC -!- DISRUPTION PHENOTYPE: Defects in seedling development, root elongation,
CC leaf expansion, flower morphogenesis and fertility due to defective
CC cytokinesis in epidermal cells. {ECO:0000269|PubMed:24179130}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87914.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; KC533769; AGI62176.1; -; mRNA.
DR EMBL; AL132963; CAB87914.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78464.1; -; Genomic_DNA.
DR EMBL; AY093166; AAM13165.1; -; mRNA.
DR EMBL; BT033150; ACF41944.1; -; mRNA.
DR PIR; T49282; T49282.
DR RefSeq; NP_190455.2; NM_114745.6. [Q8RWD5-1]
DR AlphaFoldDB; Q8RWD5; -.
DR SMR; Q8RWD5; -.
DR STRING; 3702.AT3G48860.2; -.
DR iPTMnet; Q8RWD5; -.
DR PaxDb; Q8RWD5; -.
DR PRIDE; Q8RWD5; -.
DR ProteomicsDB; 232918; -. [Q8RWD5-1]
DR EnsemblPlants; AT3G48860.2; AT3G48860.2; AT3G48860. [Q8RWD5-1]
DR GeneID; 824047; -.
DR Gramene; AT3G48860.2; AT3G48860.2; AT3G48860. [Q8RWD5-1]
DR KEGG; ath:AT3G48860; -.
DR Araport; AT3G48860; -.
DR TAIR; locus:2099423; AT3G48860.
DR eggNOG; ENOG502QQ0G; Eukaryota.
DR HOGENOM; CLU_019997_3_0_1; -.
DR InParanoid; Q8RWD5; -.
DR PhylomeDB; Q8RWD5; -.
DR PRO; PR:Q8RWD5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RWD5; baseline and differential.
DR Genevisible; Q8RWD5; AT.
DR GO; GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0000911; P:cytokinesis by cell plate formation; IMP:UniProtKB.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR040321; SCD2-like.
DR PANTHER; PTHR31762; PTHR31762; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Cell membrane;
KW Coiled coil; Cytoplasmic vesicle; Growth regulation; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..577
FT /note="Coiled-coil domain-containing protein SCD2"
FT /id="PRO_0000431669"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 67..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 206..337
FT /evidence="ECO:0000255"
FT COMPBIAS 12..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 140..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..214
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 130
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 577 AA; 63725 MW; 4D6B23503BA68A9D CRC64;
MDRRRAGSPV YGRQWSESSN STEATSPAMS PAHRKQLGGI GGFSTVKRTQ NVAAKAAAQR
LAKVMALQNK DNGDEDDEDE DEDLSLRFAS ASLKPARHAP SSISSTGSNS SNGNNNLPAV
SFAPRTRSPS PALGRNFAEQ VPSSVRSASA GRPSMSARST TPTPIPNLMP PSRVSVKTPV
SIPPLDPPTR SRDKRFFADV PSVNSKEKGD QREASALRDE LDMLQEENEN VLEKLRRAEE
KRVEAEARAK ELEKQVASLG EGVSLEAKLL SRKEAALRQR EAALNVAKQK KSGKDEEIVS
LRSELENLKD EATTAAERLQ EAESEAKSLR TMTQRMILTQ DEMEEVVLKR CWLARYWGLA
VQHGICADIA PSRQEHWSKL APLPFELVTS AAQKAKELSW DKGGNDRSKA ARDLSDLTGE
GNIESMLSVE MGLRELASLK VEDAVVLIFA QQRKLSLVRH TVSDSKGHGE SRFIDAYELG
EAEQEDVAFK QAWLMYFWGR AKLHGVEDDI AEERVQLWIS RSSGKSQTTS HDALDVERGL
IELRKLGIEQ QLWEASRREI DQLPSPTSSN HDLDADS
