SCF_CANLF
ID SCF_CANLF Reviewed; 274 AA.
AC Q06220; Q8SPM6;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Kit ligand;
DE AltName: Full=Mast cell growth factor;
DE Short=MGF;
DE AltName: Full=Stem cell factor;
DE Short=SCF;
DE AltName: Full=c-Kit ligand;
DE Contains:
DE RecName: Full=Soluble KIT ligand;
DE Short=sKITLG;
DE Contains:
DE RecName: Full=Processed kit ligand;
DE Flags: Precursor;
GN Name=KITLG; Synonyms=MGF;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=T-cell;
RX PubMed=1281786;
RA Shull R.M., Suggs S.V., Langley K.E., Okino K.H., Jacobsen F.W.,
RA Martin F.H.;
RT "Canine stem cell factor (c-kit ligand) supports the survival of
RT hematopoietic progenitors in long-term canine marrow culture.";
RL Exp. Hematol. 20:1118-1124(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-274.
RC TISSUE=Tail;
RA Schmutz S.M., Berryere T.G.;
RT "MGF sequencing in the dog aids in mapping to CFA15.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Stimulates the proliferation of mast cells. Able to augment
CC the proliferation of both myeloid and lymphoid hematopoietic
CC progenitors in bone marrow culture. Mediates also cell-cell adhesion.
CC Acts synergistically with other cytokines, probably interleukins.
CC -!- SUBUNIT: Homodimer, non-covalently linked. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P21583}.
CC Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane
CC {ECO:0000250|UniProtKB:P21583}; Single-pass type I membrane protein
CC {ECO:0000250}. Cell projection, lamellipodium
CC {ECO:0000250|UniProtKB:P21583}. Cell projection, filopodium
CC {ECO:0000250|UniProtKB:P21583}.
CC -!- SUBCELLULAR LOCATION: [Processed kit ligand]: Secreted.
CC -!- SUBCELLULAR LOCATION: [Soluble KIT ligand]: Secreted {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis.
CC -!- PTM: A soluble form is produced by proteolytic processing of the
CC extracellular domain. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SCF family. {ECO:0000305}.
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DR EMBL; S53329; AAB24619.1; -; mRNA.
DR EMBL; AY094361; AAM16280.1; -; mRNA.
DR PIR; I46929; I46929.
DR RefSeq; NP_001012753.1; NM_001012735.2.
DR AlphaFoldDB; Q06220; -.
DR SMR; Q06220; -.
DR STRING; 9615.ENSCAFP00000066357; -.
DR PaxDb; Q06220; -.
DR Ensembl; ENSCAFT00030032992; ENSCAFP00030028782; ENSCAFG00030017725.
DR Ensembl; ENSCAFT00040028995; ENSCAFP00040025188; ENSCAFG00040015574.
DR Ensembl; ENSCAFT00845032796; ENSCAFP00845025667; ENSCAFG00845018506.
DR GeneID; 403507; -.
DR KEGG; cfa:403507; -.
DR CTD; 4254; -.
DR VEuPathDB; HostDB:ENSCAFG00845018506; -.
DR eggNOG; ENOG502QTGT; Eukaryota.
DR GeneTree; ENSGT00390000018272; -.
DR HOGENOM; CLU_090207_0_0_1; -.
DR InParanoid; Q06220; -.
DR OMA; CWISVMV; -.
DR OrthoDB; 1083457at2759; -.
DR TreeFam; TF330811; -.
DR Reactome; R-CFA-1257604; PIP3 activates AKT signaling.
DR Reactome; R-CFA-1433557; Signaling by SCF-KIT.
DR Reactome; R-CFA-1433559; Regulation of KIT signaling.
DR Reactome; R-CFA-5673001; RAF/MAP kinase cascade.
DR Reactome; R-CFA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR Proteomes; UP000002254; Chromosome 15.
DR Bgee; ENSCAFG00000006091; Expressed in bone marrow and 44 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IEA:Ensembl.
DR GO; GO:0030175; C:filopodium; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030027; C:lamellipodium; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0005173; F:stem cell factor receptor binding; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0035234; P:ectopic germ cell programmed cell death; IEA:Ensembl.
DR GO; GO:0035162; P:embryonic hemopoiesis; IEA:Ensembl.
DR GO; GO:0097192; P:extrinsic apoptotic signaling pathway in absence of ligand; IEA:Ensembl.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IEA:Ensembl.
DR GO; GO:0008584; P:male gonad development; IEA:Ensembl.
DR GO; GO:0033024; P:mast cell apoptotic process; IEA:Ensembl.
DR GO; GO:0097531; P:mast cell migration; IEA:Ensembl.
DR GO; GO:0070662; P:mast cell proliferation; IEA:Ensembl.
DR GO; GO:0097324; P:melanocyte migration; IEA:Ensembl.
DR GO; GO:0002573; P:myeloid leukocyte differentiation; IEA:Ensembl.
DR GO; GO:0033026; P:negative regulation of mast cell apoptotic process; IEA:Ensembl.
DR GO; GO:0001755; P:neural crest cell migration; IEA:Ensembl.
DR GO; GO:0018108; P:peptidyl-tyrosine phosphorylation; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:1901534; P:positive regulation of hematopoietic progenitor cell differentiation; IEA:Ensembl.
DR GO; GO:1902035; P:positive regulation of hematopoietic stem cell proliferation; IEA:Ensembl.
DR GO; GO:0002687; P:positive regulation of leukocyte migration; IEA:Ensembl.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; IEA:Ensembl.
DR GO; GO:0070668; P:positive regulation of mast cell proliferation; IEA:Ensembl.
DR GO; GO:0045636; P:positive regulation of melanocyte differentiation; IEA:Ensembl.
DR GO; GO:0002763; P:positive regulation of myeloid leukocyte differentiation; IEA:Ensembl.
DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IEA:Ensembl.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IEA:Ensembl.
DR GO; GO:0042102; P:positive regulation of T cell proliferation; IEA:Ensembl.
DR GO; GO:0007265; P:Ras protein signal transduction; IEA:Ensembl.
DR GO; GO:0042098; P:T cell proliferation; IEA:Ensembl.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR003452; SCF.
DR PANTHER; PTHR11574; PTHR11574; 1.
DR Pfam; PF02404; SCF; 1.
DR PIRSF; PIRSF015599; SCF; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
PE 2: Evidence at transcript level;
KW Cell adhesion; Cell membrane; Cell projection; Cytoplasm; Cytoskeleton;
KW Disulfide bond; Glycoprotein; Growth factor; Membrane; Reference proteome;
KW Secreted; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000250"
FT CHAIN 26..274
FT /note="Kit ligand"
FT /id="PRO_0000031909"
FT CHAIN 26..191
FT /note="Soluble KIT ligand"
FT /evidence="ECO:0000250"
FT /id="PRO_0000403387"
FT CHAIN 26..?
FT /note="Processed kit ligand"
FT /id="PRO_0000292273"
FT TOPO_DOM 26..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..274
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 29..114
FT /evidence="ECO:0000250"
FT DISULFID 68..164
FT /evidence="ECO:0000250"
SQ SEQUENCE 274 AA; 30870 MW; 4182BE9AED00793B CRC64;
MKKTQTWIIT CIYLQLLLFN PLVKTKGICG KRVTDDVKDV TKLVANLPKD YKIALKYVPG
MDVLPSHCWI SVMVEQLSVS LTDLLDKFSN ISEGLSNYSI IDKLVKIVDD LVECTEGYSF
ENVKKAPKSP ELRLFTPEEF FRIFNRSIDA FKDLETVASK SSECVVSSTL SPDKDSRVSV
TKPFMLPPVA ASSLRNDSSS SNRKASNSIG DSNLQWAAMA LPAFFSLVIG FAFGALYWKK
KQPNLTRTVE NIQINEEDNE ISMLQEKERE FQEV
