SCF_FELCA
ID SCF_FELCA Reviewed; 274 AA.
AC P79169;
DT 30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Kit ligand;
DE AltName: Full=Mast cell growth factor;
DE Short=MGF;
DE AltName: Full=Stem cell factor;
DE Short=SCF;
DE AltName: Full=c-Kit ligand;
DE Contains:
DE RecName: Full=Soluble KIT ligand;
DE Short=sKITLG;
DE Flags: Precursor;
GN Name=KITLG; Synonyms=SCF;
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=8912926; DOI=10.3109/10425179609008448;
RA Dunham S.P., Onions D.E.;
RT "The cloning and sequencing of cDNAs encoding two isoforms of feline stem
RT cell factor.";
RL DNA Seq. 6:233-237(1996).
CC -!- FUNCTION: Stimulates the proliferation of mast cells. Able to augment
CC the proliferation of both myeloid and lymphoid hematopoietic
CC progenitors in bone marrow culture. Mediates also cell-cell adhesion.
CC Acts synergistically with other cytokines, probably interleukins (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer, non-covalently linked. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane {ECO:0000250}; Single-
CC pass type I membrane protein {ECO:0000250}. Secreted {ECO:0000250}.
CC Note=Also exists as a secreted soluble form (isoform 1 only).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm
CC {ECO:0000250|UniProtKB:P21583}. Cytoplasm, cytoskeleton {ECO:0000250}.
CC Cell membrane {ECO:0000250|UniProtKB:P21583}; Single-pass type I
CC membrane protein {ECO:0000250}. Cell projection, lamellipodium
CC {ECO:0000250|UniProtKB:P21583}. Cell projection, filopodium
CC {ECO:0000250|UniProtKB:P21583}.
CC -!- SUBCELLULAR LOCATION: [Soluble KIT ligand]: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P79169-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P79169-2; Sequence=VSP_006021;
CC -!- PTM: A soluble form is produced by proteolytic processing of isoform 1
CC in the extracellular domain. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SCF family. {ECO:0000305}.
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DR EMBL; D50833; BAA09445.1; -; mRNA.
DR RefSeq; NP_001009343.1; NM_001009343.1. [P79169-1]
DR AlphaFoldDB; P79169; -.
DR SMR; P79169; -.
DR STRING; 9685.ENSFCAP00000020444; -.
DR GeneID; 493937; -.
DR KEGG; fca:493937; -.
DR CTD; 4254; -.
DR eggNOG; ENOG502QTGT; Eukaryota.
DR InParanoid; P79169; -.
DR OrthoDB; 1083457at2759; -.
DR Proteomes; UP000011712; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030175; C:filopodium; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030027; C:lamellipodium; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0005173; F:stem cell factor receptor binding; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR003452; SCF.
DR PANTHER; PTHR11574; PTHR11574; 1.
DR Pfam; PF02404; SCF; 1.
DR PIRSF; PIRSF015599; SCF; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell adhesion; Cell membrane; Cell projection;
KW Cytoplasm; Cytoskeleton; Disulfide bond; Glycoprotein; Growth factor;
KW Membrane; Reference proteome; Secreted; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..274
FT /note="Kit ligand"
FT /id="PRO_0000031911"
FT CHAIN 26..191
FT /note="Soluble KIT ligand"
FT /evidence="ECO:0000250"
FT /id="PRO_0000403389"
FT TOPO_DOM 26..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..274
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 29..114
FT /evidence="ECO:0000250"
FT DISULFID 68..164
FT /evidence="ECO:0000250"
FT VAR_SEQ 175..203
FT /note="DSRVSVTKPFMLPPVAASSLRNDSSSSNR -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8912926"
FT /id="VSP_006021"
SQ SEQUENCE 274 AA; 30988 MW; C5B78DB4791237BE CRC64;
MKKTQTWIVT CIYLQLLLFN PLVKTKGLCR NRVTDDVKDV TKLVANLPKD YKIALKYVPG
MDVLPSHCWI SVMVEQLSVS LTDLLDKFSN ISEGLSNYSI IDKLVKIVDD LVECVEGHSS
ENVKKSSKSP EPRLFTPEEF FRIFNRSIDA FKDLEMVASK TSECVVSSTL SPEKDSRVSV
TKPFMLPPVA ASSLRNDSSS SNRKATNPIE DSSIQWAVMA LPACFSLVIG FAFGAFYWKK
KQPNLTRTVE NIQINEEDNE ISMLQEKERE FQEV
