ID   SCH34_STACH             Reviewed;         221 AA.
AC   C7E9V9;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Antigenic protein SchS34 {ECO:0000303|PubMed:18242638, ECO:0000303|PubMed:21109751};
DE   AltName: Full=34 kDa secretory protein {ECO:0000303|PubMed:21109751};
DE   AltName: Full=SchS34 {ECO:0000303|PubMed:18046622, ECO:0000303|PubMed:18242638, ECO:0000303|PubMed:21109751};
DE   AltName: Allergen=Sta c SchS34 {ECO:0000305};
DE   Flags: Fragment;
OS   Stachybotrys chartarum (Toxic black mold) (Stilbospora chartarum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Stachybotryaceae; Stachybotrys.
OX   NCBI_TaxID=74722 {ECO:0000312|EMBL:ACT37323.1};
RN   [1] {ECO:0000312|EMBL:ACT37323.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 13-20; 21-34; 65-76;
RP   103-110 AND 111-121, AND ALLERGEN.
RC   STRAIN=DAOM 235557 {ECO:0000303|PubMed:21109751};
RC   TISSUE=Mycelium {ECO:0000303|PubMed:21109751};
RX   PubMed=21109751; DOI=10.1159/000317243;
RA   Shi C., Smith M.L., Miller J.D.;
RT   "Characterization of human antigenic proteins SchS21 and SchS34 from
RT   Stachybotrys chartarum.";
RL   Int. Arch. Allergy Immunol. 155:74-85(2011).
RN   [2]
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=18242638; DOI=10.1016/j.jim.2007.12.021;
RA   Xu J., Liang Y., Belisle D., Miller J.D.;
RT   "Characterization of monoclonal antibodies to an antigenic protein from
RT   Stachybotrys chartarum and its measurement in house dust.";
RL   J. Immunol. Methods 332:121-128(2008).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18046622; DOI=10.1007/s11046-007-9080-1;
RA   Rand T.G., Miller J.D.;
RT   "Immunohistochemical and immunocytochemical detection of SchS34 antigen in
RT   Stachybotrys chartarum spores and spore impacted mouse lungs.";
RL   Mycopathologia 165:73-80(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18046622,
CC       ECO:0000269|PubMed:18242638}. Spore {ECO:0000269|PubMed:18046622,
CC       ECO:0000269|PubMed:18242638}. Spore wall {ECO:0000269|PubMed:18046622}.
CC       Cytoplasm {ECO:0000269|PubMed:18046622}. Note=Localizes primarily in
CC       the outer and inner layers of the spore wall and on the external wall
CC       surfaces. In the mouse lung spreaded with spores, located in spore
CC       walls along the outside surface of the outer wall and in the
CC       intercellular space surrounding spores, slowly diffusing to the
CC       surrounding tissues of mouse granuloma. {ECO:0000269|PubMed:18046622}.
CC   -!- TISSUE SPECIFICITY: Expressed in the mycelium (at protein level).
CC       {ECO:0000269|PubMed:18242638}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Natural and recombinant
CC       protein binds to IgE of patients allergic to toxic black mold fungus.
CC       {ECO:0000269|PubMed:21109751}.
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DR   EMBL; GQ258854; ACT37323.1; -; mRNA.
DR   Allergome; 9072; Sta c SchS34.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031160; C:spore wall; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Allergen; Cytoplasm; Direct protein sequencing; Glycoprotein; Secreted.
FT   CHAIN           <1..221
FT                   /note="Antigenic protein SchS34"
FT                   /id="PRO_0000455633"
FT   CARBOHYD        27
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        141
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        215
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CONFLICT        27
FT                   /note="N -> D (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        69
FT                   /note="N -> D (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT                   /evidence="ECO:0000312|EMBL:ACT37323.1"
SQ   SEQUENCE   221 AA;  23878 MW;  C7D47D69644283CD CRC64;
     AGPIASRQIV PNYPASSTSK GFHLVVNVTD PSADFTPSIN NFYVNSIHVG AALNYVGVTA
     VPGRIFYQNG TAEEIRYAQS TVISDGATPP VPFGLSLRPD EGSDVVSTAR LDAGPGTTGV
     RVSRFPEPYR FLQPETFLAC NESLAYYQGD YFTVIKQADV TVGDDGSIDY NVPDNCISVR
     LIPECTELNE LPEDAYASHE FAADTQCYDD VSALNWSEYG P