SCL3_ARATH
ID SCL3_ARATH Reviewed; 482 AA.
AC Q9LPR8; Q9XE52;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Scarecrow-like protein 3 {ECO:0000303|PubMed:10341448};
DE Short=AtSCL3 {ECO:0000303|PubMed:10341448};
DE AltName: Full=GRAS family protein 5;
DE Short=AtGRAS-5;
GN Name=SCL3 {ECO:0000303|PubMed:10341448};
GN OrderedLocusNames=At1g50420 {ECO:0000312|Araport:AT1G50420};
GN ORFNames=F11F12.22 {ECO:0000312|EMBL:AAF87889.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 158-482, AND TISSUE SPECIFICITY.
RX PubMed=10341448; DOI=10.1046/j.1365-313x.1999.00431.x;
RA Pysh L.D., Wysocka-Diller J.W., Camilleri C., Bouchez D., Benfey P.N.;
RT "The GRAS gene family in Arabidopsis: sequence characterization and basic
RT expression analysis of the SCARECROW-LIKE genes.";
RL Plant J. 18:111-119(1999).
RN [5]
RP GENE FAMILY.
RX PubMed=15316287; DOI=10.1023/b:plan.0000038256.89809.57;
RA Tian C., Wan P., Sun S., Li J., Chen M.;
RT "Genome-wide analysis of the GRAS gene family in rice and Arabidopsis.";
RL Plant Mol. Biol. 54:519-532(2004).
RN [6]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=18500650; DOI=10.1007/s11103-008-9345-1;
RA Lee M.-H., Kim B., Song S.-K., Heo J.-O., Yu N.-I., Lee S.A., Kim M.,
RA Kim D.G., Sohn S.O., Lim C.E., Chang K.S., Lee M.M., Lim J.;
RT "Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.";
RL Plant Mol. Biol. 67:659-670(2008).
RN [7]
RP INTERACTION WITH MGP/IDD3; IDD4; IDD5; BIB/IDD9 AND JKD/IDD10.
RX PubMed=24821766; DOI=10.1073/pnas.1321669111;
RA Yoshida H., Hirano K., Sato T., Mitsuda N., Nomoto M., Maeo K., Koketsu E.,
RA Mitani R., Kawamura M., Ishiguro S., Tada Y., Ohme-Takagi M., Matsuoka M.,
RA Ueguchi-Tanaka M.;
RT "DELLA protein functions as a transcriptional activator through the DNA
RT binding of the indeterminate domain family proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:7861-7866(2014).
CC -!- FUNCTION: Probable transcription factor involved in plant development.
CC {ECO:0000250}.
CC -!- SUBUNIT: Binds to zinc finger proteins MGP/IDD3, IDD4, IDD5, BIB/IDD9
CC and JKD/IDD10. {ECO:0000269|PubMed:24821766}.
CC -!- INTERACTION:
CC Q9LPR8; Q9SHF8: At1g31240; NbExp=3; IntAct=EBI-4429250, EBI-25518433;
CC Q9LPR8; Q94BY1: At3g52155; NbExp=3; IntAct=EBI-4429250, EBI-4443456;
CC Q9LPR8; A0A178UPG8: At5g52210; NbExp=3; IntAct=EBI-4429250, EBI-25518453;
CC Q9LPR8; F4I907: GLYR2; NbExp=3; IntAct=EBI-4429250, EBI-4453651;
CC Q9LPR8; O49627: ISU1; NbExp=4; IntAct=EBI-4429250, EBI-4435514;
CC Q9LPR8; Q9SLH3: RGA; NbExp=3; IntAct=EBI-4429250, EBI-963624;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, root epidermis, leaves,
CC flowers and siliques. {ECO:0000269|PubMed:10341448,
CC ECO:0000269|PubMed:18500650}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR EMBL; AC012561; AAF87889.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32546.1; -; Genomic_DNA.
DR EMBL; AY056154; AAL07233.1; -; mRNA.
DR EMBL; AY091260; AAM14199.1; -; mRNA.
DR EMBL; AF036301; AAD24404.1; -; mRNA.
DR PIR; E96540; E96540.
DR PIR; T51235; T51235.
DR RefSeq; NP_175459.1; NM_103925.6.
DR PDB; 6KPB; X-ray; 2.40 A; C=1-482.
DR PDB; 6KPD; X-ray; 3.20 A; C=1-482.
DR PDBsum; 6KPB; -.
DR PDBsum; 6KPD; -.
DR AlphaFoldDB; Q9LPR8; -.
DR SMR; Q9LPR8; -.
DR BioGRID; 26689; 23.
DR DIP; DIP-60306N; -.
DR IntAct; Q9LPR8; 16.
DR STRING; 3702.AT1G50420.1; -.
DR PaxDb; Q9LPR8; -.
DR PRIDE; Q9LPR8; -.
DR ProteomicsDB; 232697; -.
DR EnsemblPlants; AT1G50420.1; AT1G50420.1; AT1G50420.
DR GeneID; 841464; -.
DR Gramene; AT1G50420.1; AT1G50420.1; AT1G50420.
DR KEGG; ath:AT1G50420; -.
DR Araport; AT1G50420; -.
DR TAIR; locus:2008086; AT1G50420.
DR eggNOG; ENOG502QPNC; Eukaryota.
DR HOGENOM; CLU_011924_0_0_1; -.
DR InParanoid; Q9LPR8; -.
DR OMA; QMNQSTL; -.
DR OrthoDB; 928399at2759; -.
DR PhylomeDB; Q9LPR8; -.
DR PRO; PR:Q9LPR8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LPR8; baseline and differential.
DR Genevisible; Q9LPR8; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009739; P:response to gibberellin; IEP:TAIR.
DR InterPro; IPR030008; Scarecrow-like_3.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 3.
DR PANTHER; PTHR31636:SF232; PTHR31636:SF232; 3.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..482
FT /note="Scarecrow-like protein 3"
FT /id="PRO_0000350847"
FT DOMAIN 45..479
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 52..115
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 134..199
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 209..241
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 250..401
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 302..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 404..479
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 165..169
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT HELIX 47..67
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 70..83
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 90..107
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 111..116
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 127..140
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 143..158
FT /evidence="ECO:0007829|PDB:6KPB"
FT TURN 159..161
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 163..171
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 176..187
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:6KPD"
FT STRAND 194..202
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 204..221
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 225..231
FT /evidence="ECO:0007829|PDB:6KPB"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 239..242
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 248..256
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 258..262
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 328..338
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 342..349
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 358..379
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 385..393
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 395..404
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 407..409
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 417..426
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 429..432
FT /evidence="ECO:0007829|PDB:6KPB"
FT HELIX 436..446
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 448..450
FT /evidence="ECO:0007829|PDB:6KPD"
FT STRAND 454..459
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 462..467
FT /evidence="ECO:0007829|PDB:6KPB"
FT STRAND 470..480
FT /evidence="ECO:0007829|PDB:6KPB"
SQ SEQUENCE 482 AA; 54153 MW; 11BF1F8205B03039 CRC64;
MVAMFQEDNG TSSVASSPLQ VFSTMSLNRP TLLASSSPFH CLKDLKPEER GLYLIHLLLT
CANHVASGSL QNANAALEQL SHLASPDGDT MQRIAAYFTE ALANRILKSW PGLYKALNAT
QTRTNNVSEE IHVRRLFFEM FPILKVSYLL TNRAILEAME GEKMVHVIDL DASEPAQWLA
LLQAFNSRPE GPPHLRITGV HHQKEVLEQM AHRLIEEAEK LDIPFQFNPV VSRLDCLNVE
QLRVKTGEAL AVSSVLQLHT FLASDDDLMR KNCALRFQNN PSGVDLQRVL MMSHGSAAEA
RENDMSNNNG YSPSGDSASS LPLPSSGRTD SFLNAIWGLS PKVMVVTEQD SDHNGSTLME
RLLESLYTYA ALFDCLETKV PRTSQDRIKV EKMLFGEEIK NIISCEGFER RERHEKLEKW
SQRIDLAGFG NVPLSYYAML QARRLLQGCG FDGYRIKEES GCAVICWQDR PLYSVSAWRC
RK
