SCL7_ARATH
ID SCL7_ARATH Reviewed; 542 AA.
AC Q9SCR0; Q9XE54;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Scarecrow-like protein 7;
DE Short=AtSCL7;
DE AltName: Full=GRAS family protein 19;
DE Short=AtGRAS-19;
GN Name=SCL7; OrderedLocusNames=At3g50650; ORFNames=T3A5.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 431-542, AND FUNCTION.
RX PubMed=10341448; DOI=10.1046/j.1365-313x.1999.00431.x;
RA Pysh L.D., Wysocka-Diller J.W., Camilleri C., Bouchez D., Benfey P.N.;
RT "The GRAS gene family in Arabidopsis: sequence characterization and basic
RT expression analysis of the SCARECROW-LIKE genes.";
RL Plant J. 18:111-119(1999).
RN [5]
RP GENE FAMILY.
RX PubMed=15316287; DOI=10.1023/b:plan.0000038256.89809.57;
RA Tian C., Wan P., Sun S., Li J., Chen M.;
RT "Genome-wide analysis of the GRAS gene family in rice and Arabidopsis.";
RL Plant Mol. Biol. 54:519-532(2004).
RN [6]
RP GENE FAMILY, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18500650; DOI=10.1007/s11103-008-9345-1;
RA Lee M.-H., Kim B., Song S.-K., Heo J.-O., Yu N.-I., Lee S.A., Kim M.,
RA Kim D.G., Sohn S.O., Lim C.E., Chang K.S., Lee M.M., Lim J.;
RT "Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.";
RL Plant Mol. Biol. 67:659-670(2008).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia {ECO:0000303|PubMed:20616154};
RX PubMed=20616154; DOI=10.1093/jxb/erq217;
RA Ma H.S., Liang D., Shuai P., Xia X.L., Yin W.L.;
RT "The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and
RT drought tolerance in Arabidopsis thaliana.";
RL J. Exp. Bot. 61:4011-4019(2010).
CC -!- FUNCTION: Probable transcription factor involved in plant development
CC (Probable). Involved in environmental abiotic stress resistance. May
CC increase the expression of stress-responsive genes (By similarity).
CC Binds DNA in vitro (By similarity). {ECO:0000250|UniProtKB:A0A024B7I0,
CC ECO:0000250|UniProtKB:Q53K16, ECO:0000305}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q53K16}.
CC -!- INTERACTION:
CC Q9SCR0; C0SVS4: PHL11; NbExp=3; IntAct=EBI-15193551, EBI-15194203;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18500650}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, sepals, filaments of stamen,
CC and in the central cylinder of the elongation zone in root.
CC {ECO:0000269|PubMed:18500650}.
CC -!- DOMAIN: The GRAS domain is involved in DNA-binding.
CC {ECO:0000250|UniProtKB:Q53K16}.
CC -!- DISRUPTION PHENOTYPE: Reduced germination ratio by salt treatment.
CC Mannitol, an osmotic agent, together with salt strongly inhibit the
CC growth and the plants are generally unhealthier than wild-type with
CC less green cotyledons. Dramatic reduction of about 75% in root length
CC upon salt or general osmotic stress. Plants wither under drought stress
CC and the leaves have quicker water loss rate.
CC {ECO:0000269|PubMed:20616154}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000305}.
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DR EMBL; AL132979; CAB62434.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78689.1; -; Genomic_DNA.
DR EMBL; AK229252; BAF01117.1; -; mRNA.
DR EMBL; AF036304; AAD24407.1; -; mRNA.
DR PIR; T46142; T46142.
DR PIR; T51238; T51238.
DR RefSeq; NP_190634.1; NM_114925.4.
DR AlphaFoldDB; Q9SCR0; -.
DR SMR; Q9SCR0; -.
DR BioGRID; 9546; 18.
DR IntAct; Q9SCR0; 16.
DR STRING; 3702.AT3G50650.1; -.
DR PaxDb; Q9SCR0; -.
DR PRIDE; Q9SCR0; -.
DR ProteomicsDB; 232815; -.
DR EnsemblPlants; AT3G50650.1; AT3G50650.1; AT3G50650.
DR GeneID; 824228; -.
DR Gramene; AT3G50650.1; AT3G50650.1; AT3G50650.
DR KEGG; ath:AT3G50650; -.
DR Araport; AT3G50650; -.
DR TAIR; locus:2101694; AT3G50650.
DR eggNOG; ENOG502QTXA; Eukaryota.
DR HOGENOM; CLU_011924_0_5_1; -.
DR InParanoid; Q9SCR0; -.
DR OMA; WMESLIN; -.
DR OrthoDB; 690313at2759; -.
DR PhylomeDB; Q9SCR0; -.
DR PRO; PR:Q9SCR0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SCR0; baseline and differential.
DR Genevisible; Q9SCR0; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..542
FT /note="Scarecrow-like protein 7"
FT /id="PRO_0000350851"
FT DOMAIN 164..542
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 18..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..224
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 243..309
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 325..357
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 366..457
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 460..542
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 274..278
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 374..378
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 18..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..172
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 542 AA; 60779 MW; 77B213BB305D68E7 CRC64;
MAYMCTDSGN LMAIAQQLIK QKQQQQSQHQ QQEEQEQEPN PWPNPSFGFT LPGSGFSDPF
QVTNDPGFHF PHLEHHQNAA VASEEFDSDE WMESLINGGD ASQTNPDFPI YGHDPFVSFP
SRLSAPSYLN RVNKDDSASQ QLPPPPASTA IWSPSPPSPQ HPPPPPPQPD FDLNQPIFKA
IHDYARKPET KPDTLIRIKE SVSESGDPIQ RVGYYFAEAL SHKETESPSS SSSSSLEDFI
LSYKTLNDAC PYSKFAHLTA NQAILEATNQ SNNIHIVDFG IFQGIQWSAL LQALATRSSG
KPTRIRISGI PAPSLGDSPG PSLIATGNRL RDFAAILDLN FEFYPVLTPI QLLNGSSFRV
DPDEVLVVNF MLELYKLLDE TATTVGTALR LARSLNPRIV TLGEYEVSLN RVEFANRVKN
SLRFYSAVFE SLEPNLDRDS KERLRVERVL FGRRIMDLVR SDDDNNKPGT RFGLMEEKEQ
WRVLMEKAGF EPVKPSNYAV SQAKLLLWNY NYSTLYSLVE SEPGFISLAW NNVPLLTVSS
WR
