SCL7_ORYSJ
ID SCL7_ORYSJ Reviewed; 578 AA.
AC Q53K16;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=SCARECROW-LIKE protein 7 {ECO:0000303|PubMed:27081181};
DE AltName: Full=Os-SCL7 {ECO:0000303|PubMed:27081181};
GN Name=SCL7 {ECO:0000303|PubMed:27081181};
GN OrderedLocusNames=LOC_Os03g51330 {ECO:0000312|EMBL:ABF98609.1},
GN Os03g0723000 {ECO:0000312|EMBL:BAF13029.1, ECO:0000312|EMBL:BAS86141.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|EMBL:AAX95685.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6] {ECO:0007744|PDB:5HYZ}
RP X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS) OF 204-578, FUNCTION, DNA-BINDING,
RP SUBUNIT, DOMAIN, AND MUTAGENESIS OF LYS-284; ARG-478; ARG-485; LYS-549 AND
RP LYS-551.
RX PubMed=27081181; DOI=10.1105/tpc.16.00018;
RA Li S., Zhao Y., Zhao Z., Wu X., Sun L., Liu Q., Wu Y.;
RT "Crystal structure of the GRAS domain of SCARECROW-LIKE7 in Oryza sativa.";
RL Plant Cell 28:1025-1034(2016).
CC -!- FUNCTION: Probable transcription factor involved in plant development
CC (By similarity). Involved in environmental abiotic stress resistance.
CC May increase the expression of stress-responsive genes (By similarity).
CC Binds DNA in vitro (PubMed:27081181).
CC {ECO:0000250|UniProtKB:A0A024B7I0, ECO:0000250|UniProtKB:Q9SCR0,
CC ECO:0000269|PubMed:27081181}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:27081181}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:A0A024B7I0}.
CC -!- DOMAIN: The GRAS domain is involved in DNA-binding.
CC {ECO:0000269|PubMed:27081181}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000255|PROSITE-
CC ProRule:PRU01191, ECO:0000303|PubMed:27081181}.
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DR EMBL; AC146936; AAX95685.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98609.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13029.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86141.1; -; Genomic_DNA.
DR EMBL; AK064022; BAG88969.1; -; mRNA.
DR EMBL; AK101603; BAG95146.1; -; mRNA.
DR EMBL; AK102857; BAG95752.1; -; mRNA.
DR RefSeq; XP_015628849.1; XM_015773363.1.
DR PDB; 5HYZ; X-ray; 1.82 A; A=204-578.
DR PDBsum; 5HYZ; -.
DR AlphaFoldDB; Q53K16; -.
DR SMR; Q53K16; -.
DR STRING; 4530.OS03T0723000-01; -.
DR PaxDb; Q53K16; -.
DR PRIDE; Q53K16; -.
DR EnsemblPlants; Os03t0723000-01; Os03t0723000-01; Os03g0723000.
DR EnsemblPlants; Os03t0723000-02; Os03t0723000-02; Os03g0723000.
DR GeneID; 4333948; -.
DR Gramene; Os03t0723000-01; Os03t0723000-01; Os03g0723000.
DR Gramene; Os03t0723000-02; Os03t0723000-02; Os03g0723000.
DR KEGG; osa:4333948; -.
DR eggNOG; ENOG502QTXA; Eukaryota.
DR HOGENOM; CLU_011924_0_5_1; -.
DR InParanoid; Q53K16; -.
DR OMA; FSACPNR; -.
DR OrthoDB; 690313at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Nucleus; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..578
FT /note="SCARECROW-LIKE protein 7"
FT /id="PRO_0000442600"
FT DOMAIN 198..578
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 18..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 205..264
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 283..349
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 365..397
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 406..497
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 500..578
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 314..318
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 414..418
FT /note="LXXLL motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MUTAGEN 284
FT /note="K->A: Does not affect DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT MUTAGEN 284
FT /note="K->E: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT MUTAGEN 478
FT /note="R->A: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT MUTAGEN 485
FT /note="R->A: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT MUTAGEN 549
FT /note="K->A,E: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT MUTAGEN 551
FT /note="K->A: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:27081181"
FT HELIX 206..219
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 226..235
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 242..260
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 277..289
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 292..307
FT /evidence="ECO:0007829|PDB:5HYZ"
FT TURN 308..310
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 312..321
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 325..335
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 343..351
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 353..355
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 360..376
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 380..385
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 390..392
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 395..398
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 404..412
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 414..417
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 420..422
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 423..434
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 438..445
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 449..452
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 454..475
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 481..489
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 491..499
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 504..506
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 514..523
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 527..529
FT /evidence="ECO:0007829|PDB:5HYZ"
FT HELIX 535..542
FT /evidence="ECO:0007829|PDB:5HYZ"
FT TURN 543..545
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 552..557
FT /evidence="ECO:0007829|PDB:5HYZ"
FT TURN 558..560
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 561..566
FT /evidence="ECO:0007829|PDB:5HYZ"
FT STRAND 569..578
FT /evidence="ECO:0007829|PDB:5HYZ"
SQ SEQUENCE 578 AA; 62478 MW; DA75DDB01B373A68 CRC64;
MAYMCADSGN LMAIAQQVIQ QQQQQQQQQQ RHHHHHHLPP PPPPQSMAPH HHQQKHHHHH
QQMPAMPQAP PSSHGQIPGQ LAYGGGAAWP AGEHFFADAF GASAGDAVFS DLAAAADFDS
DGWMESLIGD APFQDSDLER LIFTTPPPPV PSPPPTHAAA TATATAATAA PRPEAAPALL
PQPAAATPVA CSSPSPSSAD ASCSAPILQS LLSCSRAAAT DPGLAAAELA SVRAAATDAG
DPSERLAFYF ADALSRRLAC GTGAPPSAEP DARFASDELT LCYKTLNDAC PYSKFAHLTA
NQAILEATGA ATKIHIVDFG IVQGIQWAAL LQALATRPEG KPTRIRITGV PSPLLGPQPA
ASLAATNTRL RDFAKLLGVD FEFVPLLRPV HELNKSDFLV EPDEAVAVNF MLQLYHLLGD
SDELVRRVLR LAKSLSPAVV TLGEYEVSLN RAGFVDRFAN ALSYYRSLFE SLDVAMTRDS
PERVRVERWM FGERIQRAVG PEEGADRTER MAGSSEWQTL MEWCGFEPVP LSNYARSQAD
LLLWNYDSKY KYSLVELPPA FLSLAWEKRP LLTVSAWR
