SCLLA_DROME
ID SCLLA_DROME Reviewed; 280 AA.
AC Q9VTH4; B9EQR6; Q5U1B7; Q8SY44; Q9NHN4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein scylla;
DE AltName: Full=Protein regulated in development and DNA damage response 2;
DE Short=REDD-2;
GN Name=scyl; ORFNames=CG7590;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J.,
RA Aragnol D.;
RT "Characterization of charybde and scylla, two paralogous target genes of
RT Hox and cofactors proteins in Drosophila.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION.
RX PubMed=12453409; DOI=10.1016/s1097-2765(02)00706-2;
RA Ellisen L.W., Ramsayer K.D., Johannessen C.M., Yang A., Beppu H., Minda K.,
RA Oliner J.D., McKeon F., Haber D.A.;
RT "REDD1, a developmentally regulated transcriptional target of p63 and p53,
RT links p63 to regulation of reactive oxygen species.";
RL Mol. Cell 10:995-1005(2002).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=15545626; DOI=10.1101/gad.322704;
RA Reiling J.H., Hafen E.;
RT "The hypoxia-induced paralogs Scylla and Charybdis inhibit growth by down-
RT regulating S6K activity upstream of TSC in Drosophila.";
RL Genes Dev. 18:2879-2892(2004).
RN [8]
RP FUNCTION.
RX PubMed=15632201; DOI=10.1074/jbc.c400557200;
RA Corradetti M.N., Inoki K., Guan K.-L.;
RT "The stress-inducted proteins RTP801 and RTP801L are negative regulators of
RT the mammalian target of rapamycin pathway.";
RL J. Biol. Chem. 280:9769-9772(2005).
RN [9]
RP FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16423342; DOI=10.1016/j.ydbio.2005.12.014;
RA Scuderi A., Simin K., Kazuko S.G., Metherall J.E., Letsou A.;
RT "scylla and charybde, homologues of the human apoptotic gene RTP801, are
RT required for head involution in Drosophila.";
RL Dev. Biol. 291:110-122(2006).
CC -!- FUNCTION: Inhibits cell growth by regulating the Tor pathway upstream
CC of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as cell death
CC activator during head development. {ECO:0000269|PubMed:15545626,
CC ECO:0000269|PubMed:15632201, ECO:0000269|PubMed:16423342}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expression peaks 2-8 hours after egg laying.
CC Expressed in the dorsal domains of gastrulation stage embryos. During
CC mid-embryonic developmental stages, expressed in the central nervous
CC system, the three thoracic segments and the cardiac precursor cells.
CC {ECO:0000269|PubMed:16423342}.
CC -!- INDUCTION: Expression is controlled by homeobox transcription factors.
CC At larval stages, induced by starvation, and by hypoxia in midgut and
CC fat body. {ECO:0000269|PubMed:15545626, ECO:0000269|PubMed:16423342}.
CC -!- SIMILARITY: Belongs to the DDIT4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL68220.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AF221110; AAF59841.1; -; mRNA.
DR EMBL; AE014296; AAF50074.1; -; Genomic_DNA.
DR EMBL; AY075382; AAL68220.1; ALT_SEQ; mRNA.
DR EMBL; BT015975; AAV36860.1; -; mRNA.
DR EMBL; BT057991; ACM16701.1; -; mRNA.
DR RefSeq; NP_648456.2; NM_140199.4.
DR RefSeq; NP_996043.2; NM_206321.2.
DR AlphaFoldDB; Q9VTH4; -.
DR SMR; Q9VTH4; -.
DR BioGRID; 64642; 6.
DR IntAct; Q9VTH4; 5.
DR STRING; 7227.FBpp0075899; -.
DR PaxDb; Q9VTH4; -.
DR DNASU; 39270; -.
DR EnsemblMetazoa; FBtr0076168; FBpp0075899; FBgn0041094.
DR EnsemblMetazoa; FBtr0339908; FBpp0308940; FBgn0041094.
DR GeneID; 39270; -.
DR KEGG; dme:Dmel_CG7590; -.
DR UCSC; CG7590-RA; d. melanogaster.
DR CTD; 39270; -.
DR FlyBase; FBgn0041094; scyl.
DR VEuPathDB; VectorBase:FBgn0041094; -.
DR eggNOG; ENOG502R3EE; Eukaryota.
DR GeneTree; ENSGT00530000063652; -.
DR HOGENOM; CLU_074668_1_0_1; -.
DR InParanoid; Q9VTH4; -.
DR OMA; EQGKGCH; -.
DR OrthoDB; 1588396at2759; -.
DR PhylomeDB; Q9VTH4; -.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR SignaLink; Q9VTH4; -.
DR BioGRID-ORCS; 39270; 0 hits in 3 CRISPR screens.
DR ChiTaRS; scyl; fly.
DR GenomeRNAi; 39270; -.
DR PRO; PR:Q9VTH4; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0041094; Expressed in saliva-secreting gland and 26 other tissues.
DR Genevisible; Q9VTH4; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IMP:UniProtKB.
DR GO; GO:0008219; P:cell death; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:UniProtKB.
DR GO; GO:0045926; P:negative regulation of growth; IGI:FlyBase.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:InterPro.
DR GO; GO:0032006; P:regulation of TOR signaling; IMP:UniProtKB.
DR GO; GO:0006979; P:response to oxidative stress; IMP:UniProtKB.
DR GO; GO:0019953; P:sexual reproduction; IEP:FlyBase.
DR Gene3D; 3.90.470.40; -; 1.
DR InterPro; IPR012918; RTP801-like.
DR InterPro; IPR038281; RTP801-like_C_sf.
DR PANTHER; PTHR12478; PTHR12478; 1.
DR Pfam; PF07809; RTP801_C; 1.
PE 2: Evidence at transcript level;
KW Apoptosis; Cytoplasm; Developmental protein; Reference proteome.
FT CHAIN 1..280
FT /note="Protein scylla"
FT /id="PRO_0000307211"
FT REGION 39..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..96
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 21
FT /note="K -> E (in Ref. 4; AAV36860)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="S -> P (in Ref. 1; AAF59841)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 30762 MW; 894841884CD28258 CRC64;
MKMDVIAREQ IIYGSLQGSN KNKDWTSRLP PPSAYTLDLM SKKAKTTTGG SSNGSNATAT
STTTSTSSSI KHKQPAGSSN NNVGQSQSKK TKPSGSYNSN SNYYYYAADE EEGGSADYAL
SNYDKKAVEE LSLRLLDELR AAKSRHLTCT EVSLPCDLTP SVAREIIRVS EKEPRGIRGC
TIYIEFEDEP KNSRRIASIK VDSDTVSTFE VYLTLRQDHR GWTSLLPQFM KSLARTITIS
PEYTITKNKL YSADGLGARR SYSFGSHAHR PSAAIATPTN
