ID   SCLY_XENTR              Reviewed;         431 AA.
AC   Q5U4Q9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Selenocysteine lyase;
DE            EC=4.4.1.16;
GN   Name=scly;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the decomposition of L-selenocysteine to L-alanine
CC       and elemental selenium. {ECO:0000250|UniProtKB:Q68FT9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + L-selenocysteine = A + H(+) + hydrogenselenide + L-
CC         alanine; Xref=Rhea:RHEA:11632, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:29317, ChEBI:CHEBI:57843,
CC         ChEBI:CHEBI:57972; EC=4.4.1.16;
CC         Evidence={ECO:0000250|UniProtKB:Q68FT9};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11633;
CC         Evidence={ECO:0000250|UniProtKB:Q68FT9};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:Q68FT9};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q68FT9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9JLI6}.
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH84987.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; BC084987; AAH84987.1; ALT_FRAME; mRNA.
DR   RefSeq; NP_001011164.1; NM_001011164.1.
DR   RefSeq; XP_012825815.1; XM_012970361.2.
DR   RefSeq; XP_017949356.1; XM_018093867.1.
DR   AlphaFoldDB; Q5U4Q9; -.
DR   SMR; Q5U4Q9; -.
DR   STRING; 8364.ENSXETP00000010463; -.
DR   PaxDb; Q5U4Q9; -.
DR   DNASU; 496582; -.
DR   Ensembl; ENSXETT00000026454; ENSXETP00000026454; ENSXETG00000026777.
DR   GeneID; 496582; -.
DR   KEGG; xtr:496582; -.
DR   CTD; 51540; -.
DR   Xenbase; XB-GENE-485701; scly.
DR   eggNOG; KOG1549; Eukaryota.
DR   HOGENOM; CLU_003433_0_0_1; -.
DR   InParanoid; Q5U4Q9; -.
DR   OrthoDB; 697150at2759; -.
DR   Reactome; R-XTR-2408508; Metabolism of ingested SeMet, Sec, MeSec into H2Se.
DR   Proteomes; UP000008143; Chromosome 5.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000026777; Expressed in early embryo and 16 other tissues.
DR   ExpressionAtlas; Q5U4Q9; differential.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0009000; F:selenocysteine lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR016454; Cysteine_dSase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF005572; NifS; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lyase; Pyridoxal phosphate; Reference proteome; Transferase.
FT   CHAIN           1..431
FT                   /note="Selenocysteine lyase"
FT                   /id="PRO_0000317016"
FT   ACT_SITE        367
FT                   /note="S-selanylcysteine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q68FT9"
FT   MOD_RES         239
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q68FT9"
SQ   SEQUENCE   431 AA;  46993 MW;  E70B89260137E797 CRC64;
     MADAESQNGE NHLPHKIYLD YNATTPPATE VVKAVEEALR EAWGNPSSSY TAGCKAKELI
     DTARAHVAKM VGGKPEDIIF TSGGTEANNM VLFSTVENFN STSKERQNNR VALALPHIIT
     SNVEHDSVAL PLLHLQKTHR AEITFVPVST VTGRIEVEDI ISAVRPNTCL VSIMLANNET
     GVIMPVGELS QCLASMSKER SAQGLPKILL HTDAAQALGK VEVDVQELGV NYLTIVGHKF
     YGPRIGALYV RGLGQHSSLL PMLYGGGQER NFRPGTENTP MIAGLGKAAE LVFLHCAVYE
     AHMRRIRDYL EERLEAVFED RIRLNSRFPG AERLPNTCNV SLLKPAMLGH EWLSHCQYLQ
     ASIGAACHSD RGDRPSPVLL NSGVPQEAAT SAVRLSVGRE TSQDDVDLIV RDLEQAAQLL
     GVNKKSLKKL P