SCML4_MOUSE
ID SCML4_MOUSE Reviewed; 408 AA.
AC Q80VG1; Q3TA16; Q3V358; Q5DU61; Q8CC08;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Sex comb on midleg-like protein 4;
GN Name=Scml4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Diencephalon, Head, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 47-408.
RC TISSUE=Fetal brain;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55 AND SER-65, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Putative Polycomb group (PcG) protein. PcG proteins act by
CC forming multiprotein complexes, which are required to maintain the
CC transcriptionally repressive state of homeotic genes throughout
CC development (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80VG1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80VG1-2; Sequence=VSP_033947;
CC -!- SIMILARITY: Belongs to the SCM family. {ECO:0000305}.
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DR EMBL; AK034171; BAC28615.1; -; mRNA.
DR EMBL; AK047972; BAE20663.1; -; mRNA.
DR EMBL; AK172160; BAE42854.1; -; mRNA.
DR EMBL; BC043310; AAH43310.1; -; mRNA.
DR EMBL; AK220309; BAD90230.1; -; mRNA.
DR CCDS; CCDS23816.1; -. [Q80VG1-1]
DR CCDS; CCDS87999.1; -. [Q80VG1-2]
DR RefSeq; NP_766526.3; NM_172938.3. [Q80VG1-1]
DR RefSeq; XP_006512816.1; XM_006512753.3.
DR AlphaFoldDB; Q80VG1; -.
DR SMR; Q80VG1; -.
DR BioGRID; 234479; 1.
DR STRING; 10090.ENSMUSP00000053157; -.
DR iPTMnet; Q80VG1; -.
DR PhosphoSitePlus; Q80VG1; -.
DR EPD; Q80VG1; -.
DR MaxQB; Q80VG1; -.
DR PaxDb; Q80VG1; -.
DR PRIDE; Q80VG1; -.
DR ProteomicsDB; 256929; -. [Q80VG1-1]
DR ProteomicsDB; 256930; -. [Q80VG1-2]
DR Antibodypedia; 32183; 63 antibodies from 19 providers.
DR DNASU; 268297; -.
DR Ensembl; ENSMUST00000063063; ENSMUSP00000053157; ENSMUSG00000044770. [Q80VG1-1]
DR Ensembl; ENSMUST00000105494; ENSMUSP00000101133; ENSMUSG00000044770. [Q80VG1-2]
DR GeneID; 268297; -.
DR KEGG; mmu:268297; -.
DR UCSC; uc007eza.2; mouse. [Q80VG1-1]
DR CTD; 256380; -.
DR MGI; MGI:2446140; Scml4.
DR VEuPathDB; HostDB:ENSMUSG00000044770; -.
DR eggNOG; KOG3766; Eukaryota.
DR GeneTree; ENSGT00940000157463; -.
DR HOGENOM; CLU_045829_0_0_1; -.
DR InParanoid; Q80VG1; -.
DR OMA; TEECLVN; -.
DR OrthoDB; 229086at2759; -.
DR PhylomeDB; Q80VG1; -.
DR TreeFam; TF106488; -.
DR BioGRID-ORCS; 268297; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Scml4; mouse.
DR PRO; PR:Q80VG1; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q80VG1; protein.
DR Bgee; ENSMUSG00000044770; Expressed in gastrula and 71 other tissues.
DR ExpressionAtlas; Q80VG1; baseline and differential.
DR Genevisible; Q80VG1; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.150.50; -; 1.
DR Gene3D; 3.90.1150.190; -; 1.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR033763; SCML2_RBR.
DR InterPro; IPR021987; SLED.
DR InterPro; IPR038348; SLED_sf.
DR Pfam; PF17208; RBR; 1.
DR Pfam; PF00536; SAM_1; 1.
DR Pfam; PF12140; SLED; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..408
FT /note="Sex comb on midleg-like protein 4"
FT /id="PRO_0000337172"
FT DOMAIN 340..406
FT /note="SAM"
FT REGION 274..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..302
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 55
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..58
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_033947"
FT CONFLICT 31
FT /note="A -> T (in Ref. 2; AAH43310)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 408 AA; 44445 MW; 59DDD91591D10D59 CRC64;
MQTQRVPGRK RGRPPLHSTR VQMAVHNLYS ASAASVPAVT IPKKRGRKPR YKIKSPVLMT
PLALSPPRST PEPDLSSIPQ DAATIPSLVV PEALTVCLYI NKQADVGPYL ERRKLQQLPE
RLGPERPATV LQQAVQACID CAHQPRLVFS LVKQGYRGEL VSVSASFDGK QHLRSLPVVN
SVGYVLRFLT KLCRSLLCDN LFSHLPFPGS IGASDKAQER EDGRTESAKV ATAEECLANA
VGMNRYAMDF SHRGSVTHSS SLYKRLTCGD SHLAGGPATT TSGSRTNPVP SGGSSSPGLR
LPASSPKRNG TAIEGNRCAP SPSPEVQDTR RPSSRNPSTW TVEDVVRFVK DADPEALGPH
VELFRKHEID GNALLLLRSD MIMKYLGLKL GPALKLCYHI DKLKQAKF
