SCN2B_MOUSE
ID SCN2B_MOUSE Reviewed; 215 AA.
AC Q56A07;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Sodium channel subunit beta-2;
DE Flags: Precursor;
GN Name=Scn2b; Synonyms=Gm183;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PROTEIN SEQUENCE OF 99-107, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Crucial in the assembly, expression, and functional
CC modulation of the heterotrimeric complex of the sodium channel. The
CC subunit beta-2 causes an increase in the plasma membrane surface area
CC and in its folding into microvilli. Interacts with TNR may play a
CC crucial role in clustering and regulation of activity of sodium
CC channels at nodes of Ranvier (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: The voltage-sensitive sodium channel consists of an ion
CC conducting pore forming alpha-subunit (SCN2A) regulated by one or more
CC beta subunits (SCN1B, SCN2B, SCN3B and SCN4B). SCN1B and SCN3B are non-
CC covalently associated with SCN2A. SCN2B and SCN4B are disulfide-linked
CC to SCN2A (By similarity). {ECO:0000250|UniProtKB:O60939}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sodium channel auxiliary subunit SCN2B (TC
CC 8.A.17) family. {ECO:0000305}.
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DR EMBL; BC092225; AAH92225.1; -; mRNA.
DR CCDS; CCDS40606.1; -.
DR RefSeq; NP_001014761.1; NM_001014761.2.
DR AlphaFoldDB; Q56A07; -.
DR SMR; Q56A07; -.
DR STRING; 10090.ENSMUSP00000126826; -.
DR BindingDB; Q56A07; -.
DR ChEMBL; CHEMBL4630761; -.
DR ChEMBL; CHEMBL4630764; -.
DR ChEMBL; CHEMBL4630766; -.
DR GlyGen; Q56A07; 3 sites.
DR iPTMnet; Q56A07; -.
DR PhosphoSitePlus; Q56A07; -.
DR MaxQB; Q56A07; -.
DR PaxDb; Q56A07; -.
DR PRIDE; Q56A07; -.
DR ProteomicsDB; 253412; -.
DR ABCD; Q56A07; 1 sequenced antibody.
DR Antibodypedia; 2511; 310 antibodies from 33 providers.
DR DNASU; 72821; -.
DR Ensembl; ENSMUST00000093855; ENSMUSP00000091377; ENSMUSG00000070304.
DR Ensembl; ENSMUST00000170998; ENSMUSP00000126826; ENSMUSG00000070304.
DR GeneID; 72821; -.
DR KEGG; mmu:72821; -.
DR UCSC; uc009pfg.1; mouse.
DR CTD; 6327; -.
DR MGI; MGI:106921; Scn2b.
DR VEuPathDB; HostDB:ENSMUSG00000070304; -.
DR eggNOG; ENOG502R29H; Eukaryota.
DR GeneTree; ENSGT01030000234556; -.
DR HOGENOM; CLU_090350_0_0_1; -.
DR InParanoid; Q56A07; -.
DR OMA; NCYVTNP; -.
DR OrthoDB; 1380581at2759; -.
DR PhylomeDB; Q56A07; -.
DR TreeFam; TF331728; -.
DR Reactome; R-MMU-5576892; Phase 0 - rapid depolarisation.
DR BioGRID-ORCS; 72821; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q56A07; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q56A07; protein.
DR Bgee; ENSMUSG00000070304; Expressed in cerebellum lobe and 193 other tissues.
DR ExpressionAtlas; Q56A07; baseline and differential.
DR Genevisible; Q56A07; MM.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030315; C:T-tubule; IDA:MGI.
DR GO; GO:0001518; C:voltage-gated sodium channel complex; ISO:MGI.
DR GO; GO:0017080; F:sodium channel regulator activity; ISO:MGI.
DR GO; GO:1902282; F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization; IMP:MGI.
DR GO; GO:0005248; F:voltage-gated sodium channel activity; ISO:MGI.
DR GO; GO:0086006; F:voltage-gated sodium channel activity involved in cardiac muscle cell action potential; IMP:MGI.
DR GO; GO:0061337; P:cardiac conduction; IMP:MGI.
DR GO; GO:0086002; P:cardiac muscle cell action potential involved in contraction; ISO:MGI.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR GO; GO:0010467; P:gene expression; IMP:MGI.
DR GO; GO:0086012; P:membrane depolarization during cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:0007399; P:nervous system development; IEA:Ensembl.
DR GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; ISO:MGI.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:MGI.
DR GO; GO:2000649; P:regulation of sodium ion transmembrane transporter activity; ISO:MGI.
DR GO; GO:0009408; P:response to heat; IMP:MGI.
DR GO; GO:0046684; P:response to pyrethroid; ISO:MGI.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:MGI.
DR GO; GO:0006814; P:sodium ion transport; IMP:MGI.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000920; Myelin_P0-rel.
DR InterPro; IPR029873; SCN2B.
DR PANTHER; PTHR13869; PTHR13869; 1.
DR PANTHER; PTHR13869:SF3; PTHR13869:SF3; 1.
DR Pfam; PF07686; V-set; 1.
DR PRINTS; PR00213; MYELINP0.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Ion channel; Ion transport; Membrane;
KW Phosphoprotein; Reference proteome; Signal; Sodium; Sodium channel;
KW Sodium transport; Transmembrane; Transmembrane helix; Transport;
KW Voltage-gated channel.
FT SIGNAL 1..29
FT /evidence="ECO:0000250"
FT CHAIN 30..215
FT /note="Sodium channel subunit beta-2"
FT /id="PRO_0000045177"
FT TOPO_DOM 30..159
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 32..154
FT /note="Ig-like C2-type"
FT REGION 188..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 56
FT /note="Binds SCN2A"
FT /evidence="ECO:0000250|UniProtKB:O60939"
FT SITE 135
FT /note="Binds SCN2A"
FT /evidence="ECO:0000250|UniProtKB:O60939"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P54900"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 66
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 50..127
FT /evidence="ECO:0000250|UniProtKB:O60939,
FT ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 55
FT /note="Interchain; with alpha subunit"
FT /evidence="ECO:0000250|UniProtKB:O60939,
FT ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 72..75
FT /evidence="ECO:0000250|UniProtKB:O60939"
SQ SEQUENCE 215 AA; 24228 MW; 38C1687665924FD7 CRC64;
MHRDAWLPRP AFSLTGLSLF FSLVPPGRSM EVTAPTTLSV LNGSDTRLPC TFNSCYTVNH
KQFSLNWTYQ ECNNCTEEMF LQFRMKIINL KLERFGDRVE FSGNPSKYDV SVTLKNVQLE
DEGIYNCYIT NPPDRHRGHG KIYLQVLLEV PPERDSTVAV IVGASVGGFL AVVILVLMVV
KCVRRKKEQK LSTDDLKTEE EGKMDGEGNA EDGTK
