SCN3B_RAT
ID SCN3B_RAT Reviewed; 215 AA.
AC Q9JK00;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Sodium channel subunit beta-3;
DE Flags: Precursor;
GN Name=Scn3b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND PROBABLE DISULFIDE BONDS.
RC TISSUE=Brain;
RX PubMed=10688874; DOI=10.1073/pnas.030362197;
RA Morgan K., Stevens E.B., Shaw B., Cox P., Dixon A.K., Lee K., Pinnock R.D.,
RA Highes J., Richardson P.J., Mizuguchi K., Jackson A.P.;
RT "Beta3: an additional auxiliary subunit of the voltage-sensitive sodium
RT channel that modulates channel gating with distinct kinetics.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:2308-2313(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY, AND
RP SUBUNIT.
RC STRAIN=Sprague-Dawley; TISSUE=Spinal ganglion;
RX PubMed=11922146; DOI=10.1006/mcne.2001.1039;
RA Qu Y., Curtis R., Lawson D., Gilbride K., Ge P., DiStefano P.S.,
RA Silos-Santiago I., Catterall W.A., Scheuer T.;
RT "Differential modulation of sodium channel gating and persistent sodium
RT currents by the beta1, beta2, and beta3 subunits.";
RL Mol. Cell. Neurosci. 18:570-580(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBUNIT, AND INTERACTION WITH NFASC.
RX PubMed=11470829; DOI=10.1083/jcb.200102086;
RA Ratcliffe C.F., Westenbroek R.E., Curtis R., Catterall W.A.;
RT "Sodium channel beta1 and beta3 subunits associate with neurofascin through
RT their extracellular immunoglobulin-like domain.";
RL J. Cell Biol. 154:427-434(2001).
RN [5]
RP SUBUNIT, AND INTERACTION WITH SCN10A.
RX PubMed=15178439; DOI=10.1016/j.bbrc.2004.05.026;
RA Vijayaragavan K., Powell A.J., Kinghorn I.J., Chahine M.;
RT "Role of auxiliary beta1-, beta2-, and beta3-subunits and their interaction
RT with Na(v)1.8 voltage-gated sodium channel.";
RL Biochem. Biophys. Res. Commun. 319:531-540(2004).
CC -!- FUNCTION: Modulates channel gating kinetics. Causes unique persistent
CC sodium currents. Inactivates the sodium channel opening more slowly
CC than the subunit beta-1. Its association with NFASC may target the
CC sodium channels to the nodes of Ranvier of developing axons and retain
CC these channels at the nodes in mature myelinated axons.
CC -!- SUBUNIT: The voltage-sensitive sodium channel consists of an ion
CC conducting pore forming alpha-subunit regulated by one or more beta-1,
CC beta-2, beta-3 and/or beta-4 subunits. Beta-1 and beta-3 are non-
CC covalently associated with alpha, while beta-2 and beta-4 are
CC covalently linked by disulfide bonds. Beta-1 or beta-3 subunits
CC associate with NFASC. Associates with SCN10A.
CC {ECO:0000269|PubMed:11470829, ECO:0000269|PubMed:11922146,
CC ECO:0000269|PubMed:15178439}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed broadly in neurons in the central and
CC peripheral nervous systems, but not in glia and most non-neuronal
CC cells. Weak detection in lung and adrenal gland.
CC {ECO:0000269|PubMed:11922146}.
CC -!- SIMILARITY: Belongs to the sodium channel auxiliary subunit SCN3B (TC
CC 8.A.17) family. {ECO:0000305}.
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DR EMBL; AJ243395; CAB76838.1; -; mRNA.
DR EMBL; AF378093; AAK55415.1; -; mRNA.
DR EMBL; BC070899; AAH70899.1; -; mRNA.
DR RefSeq; NP_620797.1; NM_139097.3.
DR RefSeq; XP_006243164.1; XM_006243102.2.
DR RefSeq; XP_006243165.1; XM_006243103.2.
DR AlphaFoldDB; Q9JK00; -.
DR SMR; Q9JK00; -.
DR BioGRID; 251452; 2.
DR STRING; 10116.ENSRNOP00000009367; -.
DR TCDB; 8.A.17.1.2; the na(+) channel auxiliary subunit Beta1-Beta4 (sca-Beta) family.
DR GlyGen; Q9JK00; 4 sites, 9 N-linked glycans (1 site).
DR PhosphoSitePlus; Q9JK00; -.
DR PaxDb; Q9JK00; -.
DR PRIDE; Q9JK00; -.
DR Ensembl; ENSRNOT00000087022; ENSRNOP00000071448; ENSRNOG00000057221.
DR GeneID; 245956; -.
DR KEGG; rno:245956; -.
DR UCSC; RGD:621657; rat.
DR CTD; 55800; -.
DR RGD; 621657; Scn3b.
DR eggNOG; ENOG502QWH0; Eukaryota.
DR GeneTree; ENSGT00390000018560; -.
DR HOGENOM; CLU_096296_1_0_1; -.
DR InParanoid; Q9JK00; -.
DR OMA; LIFEYRN; -.
DR OrthoDB; 1198549at2759; -.
DR PhylomeDB; Q9JK00; -.
DR Reactome; R-RNO-5576892; Phase 0 - rapid depolarisation.
DR PRO; PR:Q9JK00; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000057221; Expressed in Ammon's horn and 17 other tissues.
DR Genevisible; Q9JK00; RN.
DR GO; GO:0016021; C:integral component of membrane; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0001518; C:voltage-gated sodium channel complex; ISO:RGD.
DR GO; GO:0030018; C:Z disc; ISO:RGD.
DR GO; GO:0019871; F:sodium channel inhibitor activity; IBA:GO_Central.
DR GO; GO:0017080; F:sodium channel regulator activity; ISO:RGD.
DR GO; GO:0044325; F:transmembrane transporter binding; ISO:RGD.
DR GO; GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0086006; F:voltage-gated sodium channel activity involved in cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0086014; P:atrial cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0061337; P:cardiac conduction; ISO:RGD.
DR GO; GO:0086002; P:cardiac muscle cell action potential involved in contraction; ISO:RGD.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:RGD.
DR GO; GO:0051899; P:membrane depolarization; ISO:RGD.
DR GO; GO:0086010; P:membrane depolarization during action potential; ISO:RGD.
DR GO; GO:0086012; P:membrane depolarization during cardiac muscle cell action potential; ISO:RGD.
DR GO; GO:0007399; P:nervous system development; IEP:RGD.
DR GO; GO:0010460; P:positive regulation of heart rate; ISO:RGD.
DR GO; GO:0010765; P:positive regulation of sodium ion transport; ISO:RGD.
DR GO; GO:0072659; P:protein localization to plasma membrane; ISO:RGD.
DR GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; ISO:RGD.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:RGD.
DR GO; GO:2000649; P:regulation of sodium ion transmembrane transporter activity; ISO:RGD.
DR GO; GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; ISO:RGD.
DR GO; GO:0086015; P:SA node cell action potential; ISO:RGD.
DR GO; GO:0019233; P:sensory perception of pain; IEP:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISO:RGD.
DR GO; GO:0006814; P:sodium ion transport; IDA:RGD.
DR GO; GO:0086005; P:ventricular cardiac muscle cell action potential; ISO:RGD.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR027098; Na_channel_b1/b3.
DR InterPro; IPR027096; Na_channel_b3.
DR PANTHER; PTHR10546; PTHR10546; 1.
DR PANTHER; PTHR10546:SF1; PTHR10546:SF1; 1.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Immunoglobulin domain; Ion channel;
KW Ion transport; Membrane; Reference proteome; Signal; Sodium;
KW Sodium channel; Sodium transport; Transmembrane; Transmembrane helix;
KW Transport; Voltage-gated channel.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..215
FT /note="Sodium channel subunit beta-3"
FT /id="PRO_0000014936"
FT TOPO_DOM 25..159
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 25..138
FT /note="Ig-like C2-type"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 121
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 26..48
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 45..120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
SQ SEQUENCE 215 AA; 24799 MW; 056B488EF5EAEF4F CRC64;
MPAFNRLLPL ASLVLIYWVR VCFPVCVEVP SETEAVQGNP MKLRCISCMK REEVEATTVV
EWFYRPEGGK DFLIYEYRNG HQEVESPFQG RLQWNGSKDL QDVSITVLNV TLNDSGLYTC
NVSREFEFEA HRPFVKTTRL IPLRVTEEAG EDFTSVVSEI MMYILLVFLT LWLFIEMIYC
YRKVSKAEEA AQENASDYLA IPSENKENSV VPVEE
