SCN4B_MOUSE
ID SCN4B_MOUSE Reviewed; 228 AA.
AC Q7M729;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Sodium channel subunit beta-4;
DE Flags: Precursor;
GN Name=Scn4b; Synonyms=Gm1471;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Swiss Webster; TISSUE=Spinal ganglion;
RA Puhl H.L. III, Ikeda S.R.;
RT "cDNA cloning of the mouse sodium channel beta 4 subunit (scn4b).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION.
RX PubMed=12930796; DOI=10.1523/jneurosci.23-20-07577.2003;
RA Yu F.H., Westenbroek R.E., Silos-Santiago I., McCormick K.A., Lawson D.,
RA Ge P., Ferriera H., Lilly J., DiStefano P.S., Catterall W.A., Scheuer T.,
RA Curtis R.;
RT "Sodium channel beta4, a new disulfide-linked auxiliary subunit with
RT similarity to beta2.";
RL J. Neurosci. 23:7577-7585(2003).
CC -!- FUNCTION: Modulates channel gating kinetics. Causes negative shifts in
CC the voltage dependence of activation of certain alpha sodium channels,
CC but does not affect the voltage dependence of inactivation. Modulates
CC the susceptibility of the sodium channel to inhibition by toxic
CC peptides from spider, scorpion, wasp and sea anemone venom (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: The voltage-sensitive sodium channel consists of an ion
CC conducting pore forming alpha-subunit (SCN2A) regulated by one or more
CC beta subunits (SCN1B, SCN2B, SCN3B and SCN4B). SCN1B and SCN3B are non-
CC covalently associated with SCN2A. SCN2B and SCN4B are disulfide-linked
CC to SCN2A (By similarity). {ECO:0000250|UniProtKB:Q8IWT1}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- PTM: Contains an interchain disulfide bond with SCN2A. {ECO:0000250}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sodium channel auxiliary subunit SCN4B (TC
CC 8.A.17) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY669079; AAU05394.1; -; mRNA.
DR EMBL; AC122305; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122504; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK001031; DAA01205.1; -; mRNA.
DR CCDS; CCDS23130.1; -.
DR RefSeq; NP_001013408.1; NM_001013390.2.
DR PDB; 5AYQ; X-ray; 1.70 A; A/B=30-160.
DR PDB; 5XAX; X-ray; 2.90 A; A/B=30-160.
DR PDBsum; 5AYQ; -.
DR PDBsum; 5XAX; -.
DR AlphaFoldDB; Q7M729; -.
DR SMR; Q7M729; -.
DR STRING; 10090.ENSMUSP00000062507; -.
DR GlyConnect; 2721; 8 N-Linked glycans (3 sites).
DR GlyGen; Q7M729; 4 sites, 8 N-linked glycans (3 sites).
DR iPTMnet; Q7M729; -.
DR PhosphoSitePlus; Q7M729; -.
DR PaxDb; Q7M729; -.
DR PRIDE; Q7M729; -.
DR ProteomicsDB; 256750; -.
DR ABCD; Q7M729; 1 sequenced antibody.
DR Antibodypedia; 2645; 144 antibodies from 27 providers.
DR DNASU; 399548; -.
DR Ensembl; ENSMUST00000060125; ENSMUSP00000062507; ENSMUSG00000046480.
DR GeneID; 399548; -.
DR KEGG; mmu:399548; -.
DR UCSC; uc009pfh.1; mouse.
DR CTD; 6330; -.
DR MGI; MGI:2687406; Scn4b.
DR VEuPathDB; HostDB:ENSMUSG00000046480; -.
DR eggNOG; ENOG502QTZ6; Eukaryota.
DR GeneTree; ENSGT01030000234556; -.
DR HOGENOM; CLU_104235_0_0_1; -.
DR InParanoid; Q7M729; -.
DR OMA; EGQYICF; -.
DR OrthoDB; 1219179at2759; -.
DR PhylomeDB; Q7M729; -.
DR TreeFam; TF331728; -.
DR Reactome; R-MMU-5576892; Phase 0 - rapid depolarisation.
DR BioGRID-ORCS; 399548; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Scn4b; mouse.
DR PRO; PR:Q7M729; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q7M729; protein.
DR Bgee; ENSMUSG00000046480; Expressed in triceps brachii and 110 other tissues.
DR ExpressionAtlas; Q7M729; baseline and differential.
DR Genevisible; Q7M729; MM.
DR GO; GO:0014704; C:intercalated disc; IDA:BHF-UCL.
DR GO; GO:0031226; C:intrinsic component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0001518; C:voltage-gated sodium channel complex; ISS:UniProtKB.
DR GO; GO:0017080; F:sodium channel regulator activity; ISS:UniProtKB.
DR GO; GO:0044325; F:transmembrane transporter binding; ISO:MGI.
DR GO; GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0005248; F:voltage-gated sodium channel activity; IDA:MGI.
DR GO; GO:0086006; F:voltage-gated sodium channel activity involved in cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:0086016; P:AV node cell action potential; ISO:MGI.
DR GO; GO:0086002; P:cardiac muscle cell action potential involved in contraction; ISO:MGI.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IDA:MGI.
DR GO; GO:0086012; P:membrane depolarization during cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:0010765; P:positive regulation of sodium ion transport; ISO:MGI.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; ISO:MGI.
DR GO; GO:2000649; P:regulation of sodium ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; ISO:MGI.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IDA:MGI.
DR GO; GO:0006814; P:sodium ion transport; ISO:MGI.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000920; Myelin_P0-rel.
DR InterPro; IPR044572; Na_channel_b4.
DR PANTHER; PTHR13869; PTHR13869; 1.
DR PANTHER; PTHR13869:SF14; PTHR13869:SF14; 1.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Ion channel; Ion transport; Membrane;
KW Reference proteome; Signal; Sodium; Sodium channel; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..228
FT /note="Sodium channel subunit beta-4"
FT /id="PRO_0000014938"
FT TOPO_DOM 31..161
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 31..148
FT /note="Ig-like C2-type"
FT REGION 199..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 53..131
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 58
FT /note="Interchain; with alpha subunit"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT STRAND 32..35
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 37..44
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 62..71
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 76..84
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 92..97
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:5AYQ"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 127..134
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 144..152
FT /evidence="ECO:0007829|PDB:5AYQ"
FT STRAND 156..160
FT /evidence="ECO:0007829|PDB:5XAX"
SQ SEQUENCE 228 AA; 25192 MW; BD4839047672C911 CRC64;
MSRAGNRGNT QARWLGTGLL GLFLLPMYLS LEVSVGKATT IYAINGSSIL LPCTFSSCYG
FENLYFKWSY NNSETSRILI DGIVKNDKSD PKVRVKDDDR ITLEGSTKEK TNNISILLSD
LEFSDTGRYT CFVRNPKEKD LNNSATIFLQ VVDKLEKVDN TVTLIILAVV GGVIGLLVCI
LLLKKLITFI LKKTREKKKE CLVSSSGNDN TENGLPGSKA EEKPPTKV
