SCNNA_CHICK
ID SCNNA_CHICK Reviewed; 637 AA.
AC Q92075; P70095; Q98941;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Amiloride-sensitive sodium channel subunit alpha;
DE AltName: Full=Alpha-NaCH;
DE AltName: Full=Epithelial Na(+) channel subunit alpha;
DE Short=Alpha-ENaC;
DE AltName: Full=Nonvoltage-gated sodium channel 1 subunit alpha;
DE AltName: Full=SCNEA;
GN Name=SCNN1A; Synonyms=ENAC;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), AND TISSUE
RP SPECIFICITY.
RC STRAIN=Isa brown; TISSUE=Cochlea;
RX PubMed=9003454; DOI=10.1016/s0167-4781(96)00197-2;
RA Killick R., Richardson G.;
RT "Isolation of chicken alpha ENaC splice variants from a cochlear cDNA
RT library.";
RL Biochim. Biophys. Acta 1350:33-37(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC TISSUE=Intestine;
RA Goldstein O., Asher C., Garty H.;
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited
CC by the diuretic amiloride. Mediates the electrodiffusion of the luminal
CC sodium (and water, which follows osmotically) through the apical
CC membrane of epithelial cells. Plays an essential role in electrolyte
CC and blood pressure homeostasis, but also in airway surface liquid
CC homeostasis, which is important for proper clearance of mucus.
CC {ECO:0000250|UniProtKB:P37088}.
CC -!- SUBUNIT: Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a
CC gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in
CC some organisms and can replace the alpha/SCNN1A subunit to form an
CC alternative channel with specific properties.
CC {ECO:0000250|UniProtKB:P37088}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:P37089}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P37089}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasmic granule
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasm
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:P37089}. Cell projection, cilium,
CC flagellum {ECO:0000250|UniProtKB:P37089}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=Long;
CC IsoId=Q92075-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=Q92075-2; Sequence=VSP_006196, VSP_006197;
CC Name=3;
CC IsoId=Q92075-3; Sequence=VSP_057562;
CC -!- TISSUE SPECIFICITY: The long isoform has been found in cochlea, colon,
CC and cartilage. The short isoform is only found in cochlea.
CC {ECO:0000269|PubMed:9003454}.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. SCNN1A subfamily. {ECO:0000305}.
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DR EMBL; U62902; AAB50550.1; -; mRNA.
DR EMBL; U62903; AAB50551.1; -; mRNA.
DR EMBL; U62904; AAB50552.1; -; mRNA.
DR EMBL; U58475; AAB04954.1; -; mRNA.
DR RefSeq; NP_990476.2; NM_205145.2. [Q92075-1]
DR AlphaFoldDB; Q92075; -.
DR SMR; Q92075; -.
DR STRING; 9031.ENSGALP00000022994; -.
DR PaxDb; Q92075; -.
DR GeneID; 396050; -.
DR KEGG; gga:396050; -.
DR CTD; 6337; -.
DR VEuPathDB; HostDB:geneid_396050; -.
DR eggNOG; KOG4294; Eukaryota.
DR InParanoid; Q92075; -.
DR PhylomeDB; Q92075; -.
DR PRO; PR:Q92075; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0060170; C:ciliary membrane; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR GO; GO:0034706; C:sodium channel complex; ISS:UniProtKB.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IBA:GO_Central.
DR GO; GO:0050891; P:multicellular organismal water homeostasis; ISS:UniProtKB.
DR GO; GO:0055078; P:sodium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR001873; ENaC.
DR InterPro; IPR004724; ENaC_chordates.
DR InterPro; IPR020903; ENaC_CS.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
DR TIGRFAMs; TIGR00859; ENaC; 1.
DR PROSITE; PS01206; ASC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cell projection; Cilium; Cytoplasm;
KW Cytoplasmic vesicle; Flagellum; Ion channel; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium channel; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..637
FT /note="Amiloride-sensitive sodium channel subunit alpha"
FT /id="PRO_0000181265"
FT TOPO_DOM 1..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT TRANSMEM 77..97
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..548
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT TRANSMEM 549..569
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 570..637
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..14
FT /note="Missing (in isoform 3)"
FT /id="VSP_057562"
FT VAR_SEQ 402..434
FT /note="CIRSCFQLNMVKRCSCAYYFYPLPDGAEYCDYT -> TCDHQFRASFFASFP
FT GMLQSPNSRACPRAMLIR (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:9003454"
FT /id="VSP_006196"
FT VAR_SEQ 435..637
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000303|PubMed:9003454"
FT /id="VSP_006197"
FT CONFLICT 49..50
FT /note="DV -> EL (in Ref. 2; AAB04954)"
FT /evidence="ECO:0000305"
FT CONFLICT 578
FT /note="Q -> R (in Ref. 2; AAB04954)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 637 AA; 73236 MW; 5D15E616373971DA CRC64;
MGTASRGGSV KAEKMPEGEK TRQCKQETEQ QQKEDEREGL IEFYGSYQDV FQFFCSNTTI
HGAIRLVCSK KNKMKTAFWS VLFILTFGLM YWQFGILYRE YFSYPVNLNL NLNSDRLTFP
AVTLCTLNPY RYSAIRKKLD ELDQITHQTL LDLYDYNMSL ARSDGSAQFS HRRTSRSLLH
HVQRHPLRRQ KRDNLVSLPE NSPSVDKNDW KIGFVLCSEN NEDCFHQTYS SGVDAVREWY
SFHYINILAQ MPDAKDLDES DFENFIYACR FNEATCDKAN YTHFHHPLYG NCYTFNDNSS
SLWTSSLPGI NNGLSLVVRT EQNDFIPLLS TVTGARVMVH DQNEPAFMDD GGFNVRPGIE
TSISMRKEMT ERLGGSYSDC TEDGSDVPVQ NLYSSRYTEQ VCIRSCFQLN MVKRCSCAYY
FYPLPDGAEY CDYTKHVAWG YCYYKLLAEF KADVLGCFHK CRKPCKMTEY QLSAGYSRWP
SAVSEDWVFY MLSQQNKYNI TSKRNGVAKV NIFFEEWNYK TNGESPAFTV VTLLSQLGNQ
WSLWFGSSVL SVMELAELIL DFTVITFILA FRWFRSKQWH SSPAPPPNSH DNTAFQDEAS
GLDAPHRFTV EAVVTTLPSY NSLEPCGPSK DGETGLE
