SCNNA_PELSI
ID SCNNA_PELSI Reviewed; 642 AA.
AC K7GET2;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 1.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Amiloride-sensitive sodium channel subunit alpha;
DE AltName: Full=Alpha-NaCH;
DE AltName: Full=Epithelial Na(+) channel subunit alpha;
DE Short=Alpha-ENaC;
DE AltName: Full=Nonvoltage-gated sodium channel 1 subunit alpha;
DE AltName: Full=SCNEA;
GN Name=SCNN1A;
OS Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Trionychia;
OC Trionychidae; Pelodiscus.
OX NCBI_TaxID=13735;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Soft-shell turtle genome consortium;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited
CC by the diuretic amiloride. Mediates the electrodiffusion of the luminal
CC sodium (and water, which follows osmotically) through the apical
CC membrane of epithelial cells. Plays an essential role in electrolyte
CC and blood pressure homeostasis, but also in airway surface liquid
CC homeostasis, which is important for proper clearance of mucus.
CC {ECO:0000250|UniProtKB:P37088}.
CC -!- SUBUNIT: Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a
CC gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in
CC some organisms and can replace the alpha/SCNN1A subunit to form an
CC alternative channel with specific properties.
CC {ECO:0000250|UniProtKB:P37088}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:P37089}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P37089}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasmic granule
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasm
CC {ECO:0000250|UniProtKB:P37088}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:P37089}. Cell projection, cilium,
CC flagellum {ECO:0000250|UniProtKB:P37089}.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. SCNN1A subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AGCU01093992; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01093993; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01093994; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006124236.1; XM_006124174.1.
DR AlphaFoldDB; K7GET2; -.
DR SMR; K7GET2; -.
DR STRING; 13735.ENSPSIP00000018793; -.
DR Ensembl; ENSPSIT00000018879; ENSPSIP00000018793; ENSPSIG00000016541.
DR GeneID; 102458615; -.
DR KEGG; pss:102458615; -.
DR CTD; 6337; -.
DR eggNOG; KOG4294; Eukaryota.
DR GeneTree; ENSGT00940000160952; -.
DR HOGENOM; CLU_020415_0_0_1; -.
DR OMA; EKTRQCK; -.
DR OrthoDB; 686369at2759; -.
DR TreeFam; TF330663; -.
DR Proteomes; UP000007267; Unassembled WGS sequence.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0060170; C:ciliary membrane; IEA:Ensembl.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; IEA:Ensembl.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:Ensembl.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0034706; C:sodium channel complex; IEA:Ensembl.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IEA:Ensembl.
DR GO; GO:0050699; F:WW domain binding; IEA:Ensembl.
DR GO; GO:0071468; P:cellular response to acidic pH; IEA:Ensembl.
DR GO; GO:0036254; P:cellular response to amiloride; IEA:Ensembl.
DR GO; GO:0006883; P:cellular sodium ion homeostasis; IEA:Ensembl.
DR GO; GO:0050891; P:multicellular organismal water homeostasis; IEA:Ensembl.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IEA:Ensembl.
DR GO; GO:0035725; P:sodium ion transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR001873; ENaC.
DR InterPro; IPR004724; ENaC_chordates.
DR InterPro; IPR020903; ENaC_CS.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
DR TIGRFAMs; TIGR00859; ENaC; 1.
DR PROSITE; PS01206; ASC; 1.
PE 3: Inferred from homology;
KW Cell membrane; Cell projection; Cilium; Cytoplasm; Cytoplasmic vesicle;
KW Flagellum; Ion channel; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium channel; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..642
FT /note="Amiloride-sensitive sodium channel subunit alpha"
FT /id="PRO_0000432910"
FT TOPO_DOM 1..81
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT TRANSMEM 82..102
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..553
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT TRANSMEM 554..574
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..642
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P37089"
FT REGION 170..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..608
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..197
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 642 AA; 73892 MW; C32BF6DF0CA49C21 CRC64;
MHQVVTVKAE KVPMGKRLRR CKQEAENQQK VEEVAEKLEK EHEGLIEFHK SYHELFQFFC
NNTTIHGAIR LVCSKRNKMK TAFWSVLFFL TFGLMYWQFG ILYREYFSFP VNLNLNLNSD
KLTFPAVTLC TLNPYRYSAV RKELDELDRI THQTLMDLYN YSMSQVQSNG AAQSSQKRSQ
RSLSHHVQRH PLRRRKRNEP VSLKGNSPPV DKSDWKIGFI LCNETNEDCF HQTYSSGVDA
VREWYSFHYI NILARMPDTK ALDESNFESF IYACRFNEVT CDKANYTHFH HPLYGNCYTF
NDRNNSLWTS SLPGINNGLS LLVRTEQNDY IPLLSTVTGA RVMVHEQNEP AFMDDGGFNV
RPGIETSISM RKETTMLLGG SYSDCTEDGS DVPVQNLYSS RYTEQVCIRS CFQIHMVKRC
GCAYYFYPLP PGAEYCDYTK HIAWGYCYYK LQVEFKSNIL GCFSKCRKPC EVTKYQLSAG
YSHWPSAVSE NWVFHILSQQ NKYNITSKRN GVAKVNIFFE EWKYKTNGES PAFTVVTLLS
QLGNQWSLWF GSSVLSVVEL AELILDFIAI TIILSFKRFR SRQVPAPSVP PPGAHDNTAF
QSEPADPSAP HRFTVEAVVT TLPSYNSLEP CRRDGEAVIG LE
