ID   SCNNG_NEOFS             Reviewed;         653 AA.
AC   H1AFJ7;
DT   27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Amiloride-sensitive sodium channel subunit gamma;
DE   AltName: Full=Epithelial Na(+) channel subunit gamma;
DE            Short=Gamma-ENaC;
DE   AltName: Full=Gamma-NaCH;
DE   AltName: Full=Nonvoltage-gated sodium channel 1 subunit gamma;
DE   AltName: Full=SCNEG;
GN   Name=scnn1g; Synonyms=enacgamma {ECO:0000312|EMBL:BAL46408.1};
OS   Neoceratodus forsteri (Australian lungfish) (Ceratodus forsteri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Dipnomorpha; Ceratodontiformes; Ceratodontoidei; Ceratodontidae;
OC   Neoceratodus.
OX   NCBI_TaxID=7892;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC   TISSUE=Gill;
RX   PubMed=23055064; DOI=10.1098/rspb.2012.1945;
RA   Uchiyama M., Maejima S., Yoshie S., Kubo Y., Konno N., Joss J.M.P.;
RT   "The epithelial sodium channel in the Australian lungfish, Neoceratodus
RT   forsteri (Osteichthyes: Dipnoi).";
RL   Proc. R. Soc. B 279:4795-4802(2012).
CC   -!- FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited
CC       by the diuretic amiloride. Mediates the electrodiffusion of the luminal
CC       sodium (and water, which follows osmotically) through the apical
CC       membrane of epithelial cells. Plays an essential role in electrolyte
CC       and blood pressure homeostasis, but also in airway surface liquid
CC       homeostasis, which is important for proper clearance of mucus.
CC       {ECO:0000269|PubMed:23055064}.
CC   -!- SUBUNIT: Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a
CC       gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in
CC       some organisms and can replace the alpha/SCNN1A subunit to form an
CC       alternative channel with specific properties.
CC       {ECO:0000250|UniProtKB:P51170}.
CC   -!- SUBCELLULAR LOCATION: Apical cell membrane
CC       {ECO:0000250|UniProtKB:P37091}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P37089}. Note=Apical membrane of epithelial
CC       cells. {ECO:0000250|UniProtKB:P37091}.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in gill, liver, kidney and
CC       rectum and more weakly in heart, muscle and intestine.
CC       {ECO:0000269|PubMed:23055064}.
CC   -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC       1.A.6) family. SCNN1G subfamily. {ECO:0000305}.
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DR   EMBL; AB675924; BAL46408.1; -; mRNA.
DR   AlphaFoldDB; H1AFJ7; -.
DR   SMR; H1AFJ7; -.
DR   GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR   GO; GO:0034706; C:sodium channel complex; IC:UniProtKB.
DR   GO; GO:0015280; F:ligand-gated sodium channel activity; IEA:InterPro.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   GO; GO:0050909; P:sensory perception of taste; IEA:UniProtKB-KW.
DR   GO; GO:0035725; P:sodium ion transmembrane transport; IDA:UniProtKB.
DR   InterPro; IPR001873; ENaC.
DR   InterPro; IPR004724; ENaC_chordates.
DR   InterPro; IPR020903; ENaC_CS.
DR   PANTHER; PTHR11690; PTHR11690; 1.
DR   Pfam; PF00858; ASC; 1.
DR   PRINTS; PR01078; AMINACHANNEL.
DR   TIGRFAMs; TIGR00859; ENaC; 1.
DR   PROSITE; PS01206; ASC; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Sensory transduction;
KW   Sodium; Sodium channel; Sodium transport; Taste; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..653
FT                   /note="Amiloride-sensitive sodium channel subunit gamma"
FT                   /id="PRO_0000433088"
FT   TOPO_DOM        1..54
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P37089"
FT   TRANSMEM        55..75
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        76..546
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:P37089"
FT   TRANSMEM        547..567
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        568..653
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P37089"
FT   REGION          582..608
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   653 AA;  74712 MW;  94E1D86182709C28 CRC64;
     MGHGRRISES IKKQLPVTGP EAPTVKNLMD WYLNNTNTHG CRRIAVSRGY LRRWIWICFT
     VSSVGMIFWQ WTLLLMSYYT VSVSVTVQFQ TLPFPAVTIC NINPYRKNAT SALLEELDKQ
     TKLILKELYT SCTGCSNRKL RSVLLNEAPE EDSGVAKLLQ DMPLMKFEVI KEDHVIVSEL
     SSNRQYRINN TFITRMYNNM DLATVGEQVG FKICDANKSN CIIYTFNSGV TAILEWYRLN
     YLNIMAQIPN EKKLEMGYSA DDLIVTCMYD GQSCDSRNFT LFQHPLHGNC YTFNSGDDGN
     ILQTLTGGSE YGLKLTLYLE NDDYNPYLFT SMGAKIIVHD QTEYPLVDDV GLEIQTATET
     LIGLQVTTSA KLSKPYSDCT MDGSDVLEQN LYNTSYSLQI CLHSCFQTEM ISNCGCAYYE
     QPLPSGAEYC YYEKYPGWIY CYYQLQDKFV NERLACQDIC KETCNSKDWD LTKSLARWPS
     VASKDWVLNL LNWERGLNNT LNKNDLASIA IFYQDLNLRS LSESPANSIA TLLSNMGGQL
     GLWMSCSIVC FLEMWEVFLV DILTIIARYW LHRGRQWWRK RKERQMQQPS PPDHDTGHHN
     PVCIDDEDPP TFHTAMQLPC VQTGPVPSTP PPQYNALRIQ SVFDEQVSDT EVN