SCO3_ARATH
ID SCO3_ARATH Reviewed; 644 AA.
AC Q8GXD9; Q3EB40; Q9LJN9;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein SNOWY COTYLEDON 3;
DE AltName: Full=QWRF motif-containing protein 1;
GN Name=SCO3; Synonyms=QWRF1; OrderedLocusNames=At3g19570; ORFNames=MMB12.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION, MUTAGENESIS OF GLY-8, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, INDUCTION BY NORFLURAZON, DISRUPTION PHENOTYPE, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=20978221; DOI=10.1105/tpc.110.074781;
RA Albrecht V., Simkova K., Carrie C., Delannoy E., Giraud E., Whelan J.,
RA Small I.D., Apel K., Badger M.R., Pogson B.J.;
RT "The cytoskeleton and the peroxisomal-targeted snowy cotyledon3 protein are
RT required for chloroplast development in Arabidopsis.";
RL Plant Cell 22:3423-3438(2010).
CC -!- FUNCTION: Probable microtubule-associated peroxisomal protein required
CC for chloroplast biogenesis and for the formation of the prolamellar
CC body and prothylakoids in etioplasts. Not involved in peroxisomal
CC metabolism, including mobilization of storage compounds during
CC germination, fatty acid beta-oxydation or photorespiration.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:20978221}.
CC Note=Located at discrete small spots potentially associated with the
CC surface of the peroxisome.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8GXD9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8GXD9-2; Sequence=VSP_053219, VSP_053220;
CC -!- TISSUE SPECIFICITY: Expressed in young developing tissues, such as
CC seedlings, roots, flowers, buds and young siliques, and to a lesser
CC extent in mature green tissues. {ECO:0000269|PubMed:20978221}.
CC -!- INDUCTION: Up-regulated by norflurazon. {ECO:0000269|PubMed:20978221}.
CC -!- DISRUPTION PHENOTYPE: Embryo lethality. {ECO:0000269|PubMed:20978221}.
CC -!- SIMILARITY: Belongs to the QWRF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02541.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP000417; BAB02541.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76259.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76260.1; -; Genomic_DNA.
DR EMBL; AK118290; BAC42908.1; -; mRNA.
DR EMBL; BT005534; AAO63954.1; -; mRNA.
DR PIR; T52384; T52384.
DR RefSeq; NP_188591.2; NM_112847.3. [Q8GXD9-2]
DR RefSeq; NP_850614.1; NM_180283.5. [Q8GXD9-1]
DR AlphaFoldDB; Q8GXD9; -.
DR BioGRID; 6827; 2.
DR IntAct; Q8GXD9; 2.
DR STRING; 3702.AT3G19570.2; -.
DR iPTMnet; Q8GXD9; -.
DR PaxDb; Q8GXD9; -.
DR PRIDE; Q8GXD9; -.
DR ProteomicsDB; 226604; -. [Q8GXD9-1]
DR EnsemblPlants; AT3G19570.1; AT3G19570.1; AT3G19570. [Q8GXD9-2]
DR EnsemblPlants; AT3G19570.2; AT3G19570.2; AT3G19570. [Q8GXD9-1]
DR GeneID; 821494; -.
DR Gramene; AT3G19570.1; AT3G19570.1; AT3G19570. [Q8GXD9-2]
DR Gramene; AT3G19570.2; AT3G19570.2; AT3G19570. [Q8GXD9-1]
DR KEGG; ath:AT3G19570; -.
DR Araport; AT3G19570; -.
DR TAIR; locus:2091166; AT3G19570.
DR eggNOG; ENOG502QYE5; Eukaryota.
DR InParanoid; Q8GXD9; -.
DR OrthoDB; 655703at2759; -.
DR PhylomeDB; Q8GXD9; -.
DR PRO; PR:Q8GXD9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8GXD9; baseline and differential.
DR Genevisible; Q8GXD9; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IDA:TAIR.
DR GO; GO:0005880; C:nuclear microtubule; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR InterPro; IPR007573; QWRF.
DR Pfam; PF04484; QWRF; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Peroxisome; Reference proteome.
FT CHAIN 1..644
FT /note="Protein SNOWY COTYLEDON 3"
FT /id="PRO_0000423626"
FT REGION 1..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 162..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 463..466
FT /note="QWRF motif"
FT COMPBIAS 50..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..190
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..381
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..414
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 624..627
FT /note="VAAM -> HASC (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053219"
FT VAR_SEQ 628..644
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053220"
FT MUTAGEN 8
FT /note="G->E: In sco3-1; chlorotic cotyledons with impaired
FT chloroplast and etioplast development."
FT /evidence="ECO:0000269|PubMed:20978221"
SQ SEQUENCE 644 AA; 70365 MW; 40E2B571122D13F9 CRC64;
MVAAIPQGAA ISNTDSKNPP PRDRQDKPQL TANNGGLQRR PRAAKNVPSR YLSPSPSHST
TTTTTTATST STSSSSSVIL RSSKRYPSPL LSRTTNSASN LVYTPSSLPK RSQSVDRRRP
SAVSDTRTEM SAATKMLITS TRSLSVSFQG EAFSFPISKK KETATPVSHR KCTPERRRAT
PVRDQRENSK PVDQQLWPGA SRRGSSESVV PNSLSRSVDS DSDDGRKLGS GFVGRSMLQH
SQSSRVSGDG RLNLGFVGGD GMLEMRDENK ARQSTHPRLA SSVSCDFTAS DTDSVSSGST
NGAHECGSGE VSKTRSLPRN GMASTKFWQE TNSRLRRMQD PGSPQCSSPS SRISSISSKF
SQSKRFSSDS PLTSSPRGMT SPIRGATRPA SPSKLWATAT SAPARTSSSP SRVRNGVSEQ
MNAYNRTLPS ILCFSADIRR GKIGEDRVMD AHLLRLLYNR DLQWRFANAR ADSTLMVQRL
SAEKILWNAW VSISELRHSV TLKRIKLLLM RQKLKLASIL KEQMCYLEEW SLLDRNHSNS
LSGATEALKA STLRLPVSGK AVVDIQDLKH AVSSAVDVMH AMVSSIFSLT SKVEEMNSVM
AEMVNITGKE EVLLEQCQGF LTRVAAMQVT DCSMKTHIIQ LSRL
