SCP28_ARATH
ID SCP28_ARATH Reviewed; 462 AA.
AC Q8S8K6;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Serine carboxypeptidase-like 28;
DE EC=3.4.16.-;
DE Flags: Precursor;
GN Name=SCPL28; OrderedLocusNames=At2g35770; ORFNames=T20F21.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, TISSUE SPECIFICITY, AND NOMENCLATURE.
RX PubMed=15908604; DOI=10.1104/pp.104.057950;
RA Fraser C.M., Rider L.W., Chapple C.;
RT "An expression and bioinformatics analysis of the Arabidopsis serine
RT carboxypeptidase-like gene family.";
RL Plant Physiol. 138:1136-1148(2005).
CC -!- FUNCTION: Probable carboxypeptidase. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots and senescent leaves.
CC {ECO:0000269|PubMed:15908604}.
CC -!- SIMILARITY: Belongs to the peptidase S10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM15112.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BX820274; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC006068; AAM15112.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC09159.1; -; Genomic_DNA.
DR EMBL; BX820274; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G84772; G84772.
DR RefSeq; NP_181120.2; NM_129135.3.
DR AlphaFoldDB; Q8S8K6; -.
DR SMR; Q8S8K6; -.
DR ESTHER; arath-SCP28; Carboxypeptidase_S10.
DR MEROPS; S10.A28; -.
DR PaxDb; Q8S8K6; -.
DR PRIDE; Q8S8K6; -.
DR ProteomicsDB; 232821; -.
DR EnsemblPlants; AT2G35770.1; AT2G35770.1; AT2G35770.
DR GeneID; 818149; -.
DR Gramene; AT2G35770.1; AT2G35770.1; AT2G35770.
DR KEGG; ath:AT2G35770; -.
DR Araport; AT2G35770; -.
DR TAIR; locus:2058734; AT2G35770.
DR eggNOG; KOG1282; Eukaryota.
DR HOGENOM; CLU_008523_13_0_1; -.
DR InParanoid; Q8S8K6; -.
DR OMA; HLRIWIF; -.
DR OrthoDB; 625787at2759; -.
DR PhylomeDB; Q8S8K6; -.
DR PRO; PR:Q8S8K6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8K6; baseline and differential.
DR Genevisible; Q8S8K6; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004185; F:serine-type carboxypeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001563; Peptidase_S10.
DR InterPro; IPR018202; Ser_caboxypep_ser_AS.
DR PANTHER; PTHR11802; PTHR11802; 1.
DR Pfam; PF00450; Peptidase_S10; 1.
DR PRINTS; PR00724; CRBOXYPTASEC.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00131; CARBOXYPEPT_SER_SER; 1.
PE 2: Evidence at transcript level;
KW Carboxypeptidase; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..462
FT /note="Serine carboxypeptidase-like 28"
FT /id="PRO_0000274643"
FT ACT_SITE 186
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10074"
FT ACT_SITE 379
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10074"
FT ACT_SITE 434
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10074"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 334
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 93..342
FT /evidence="ECO:0000250"
FT DISULFID 254..266
FT /evidence="ECO:0000250"
FT DISULFID 290..311
FT /evidence="ECO:0000250"
FT CONFLICT 239
FT /note="H -> N (in Ref. 3; BX820274)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="Y -> H (in Ref. 3; BX820274)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="C -> R (in Ref. 3; BX820274)"
FT /evidence="ECO:0000305"
FT CONFLICT 455
FT /note="H -> N (in Ref. 3; BX820274)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 52098 MW; EB574420E297DAC7 CRC64;
MMITKKLYQC MCLLCMVIAL LDVVSSDDAK EQKMKDKIIS LPGQPPNLNF SQFSGYVTVD
PAAGRALFYW LTEAPRPSGT KPLVLWLNGG PGCSSIAYGA SEEVGPFRVN PDGKTLRLNL
YAWNKVANVL FLDSPAGVGF SYTNTSSDEL TVGDKRTGED AYRFLVRWLE RFPEYKERAF
YIAGESYAGH YIPELAQLIV NRNKGAKNPT INLKGILMGN PLVDDYNDNK GMRDYWWNHG
LISDESYNDL TKWCLNDSIL FPKLNCNAAL NQALSEFGDI DPYNINSPAC TTHASSNEWM
QAWRYRGNDE CVVGYTRKYM NDPNVHKSFH ARLNGSTPWT PCSRVIRKNW KDSPKSMLPI
IKNLLQAHLR IWIFSGDSDA VLPLSGTRHS INAMKLKSSK RWYPWYHSHG LVGGWSQVYE
DGLLTYTTVR AAGHEVPLSQ PRLALFLFTH FLANHSLPSS PS
