SCP2_ARATH
ID SCP2_ARATH Reviewed; 123 AA.
AC Q9FMN0;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 143.
DE RecName: Full=Sterol carrier protein 2 {ECO:0000303|PubMed:15456765};
DE Short=AtSCP2 {ECO:0000303|PubMed:15456765};
GN Name=SCP2 {ECO:0000303|PubMed:15456765};
GN OrderedLocusNames=At5g42890 {ECO:0000312|Araport:AT5G42890};
GN ORFNames=MBD2.8 {ECO:0000312|EMBL:BAB09190.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=14701923; DOI=10.1093/pcp/pcg173;
RA Kamada T., Nito K., Hayashi H., Mano S., Hayashi M., Nishimura M.;
RT "Functional differentiation of peroxisomes revealed by expression profiles
RT of peroxisomal genes in Arabidopsis thaliana.";
RL Plant Cell Physiol. 44:1275-1289(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15456765; DOI=10.1074/jbc.m405099200;
RA Edqvist J., Roennberg E., Rosenquist S., Blomqvist K., Viitanen L.,
RA Salminen T.A., Nylund M., Tuuf J., Mattjus P.;
RT "Plants express a lipid transfer protein with high similarity to mammalian
RT sterol carrier protein-2.";
RL J. Biol. Chem. 279:53544-53553(2004).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND INDUCTION BY DARKNESS.
RC STRAIN=cv. Columbia;
RX PubMed=18687588; DOI=10.1093/jxb/ern201;
RA Zheng B.S., Roennberg E., Viitanen L., Salminen T.A., Lundgren K.,
RA Moritz T., Edqvist J.;
RT "Arabidopsis sterol carrier protein-2 is required for normal development of
RT seeds and seedlings.";
RL J. Exp. Bot. 59:3485-3499(2008).
CC -!- FUNCTION: Enhances the transfer of lipids between membranes in vitro
CC (PubMed:15456765). Active on phosphatidylcholine (PC), 1-palmitoyl 2-
CC oleoyl phosphatidylcholine (POPC) and ergosterol, and, to a lower
CC extent, dimyristoyl phosphatidic acid, stigmasterol, desmosterol, beta-
CC sitosterol and steryl glucoside (PubMed:15456765). Inactive or poorly
CC active on palmitic acid, stearoyl-coenzyme A, cholesterol,
CC glucosylceramide and ceramide (PubMed:15456765). Required during seeds
CC and seedlings development (PubMed:18687588).
CC {ECO:0000269|PubMed:15456765, ECO:0000269|PubMed:18687588}.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15456765}.
CC -!- TISSUE SPECIFICITY: Expressed in most tissues including seedlings,
CC cotyledons, inflorescence, leaves, stems, roots, siliques and flower
CC buds, with the highest levels in floral tissues and in maturing seeds.
CC {ECO:0000269|PubMed:14701923, ECO:0000269|PubMed:15456765,
CC ECO:0000269|PubMed:18687588}.
CC -!- DEVELOPMENTAL STAGE: In seeds, observed mainly in the endosperm and the
CC embryo (PubMed:18687588). In seedlings, detected in vascular tissues
CC and hydathodes of cotyledons (PubMed:18687588). Later expressed in
CC trichomes of rosette leaves (PubMed:18687588). In flowers, accumulates
CC in the receptacles, in vascular tissues of sepals and petals, in the
CC style and stigma of the carpels, and in anthers, filaments, and pollen
CC (PubMed:18687588). Also present in siliques funiculi (PubMed:18687588).
CC {ECO:0000269|PubMed:18687588}.
CC -!- INDUCTION: Accumulates at higher levels in dark-grown seedlings
CC (PubMed:18687588). Induced by sucrose (PubMed:18687588).
CC {ECO:0000269|PubMed:18687588}.
CC -!- DISRUPTION PHENOTYPE: Altered seed morphology and delayed seed
CC germination as well as inhibited root elongation in the dark; an
CC exogenous carbon source is required to recover from the delayed
CC seedling establishment. Reduced levels of glutamine, pyroglutamic acid,
CC aspartate, beta-alanine, fumaric acid, glyceraldehyde and ribose, but
CC increased accumulation of serine, glycine, asparagine, 3-cyanoalanine
CC and 5-methylthiopentanitrile. {ECO:0000269|PubMed:18687588}.
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DR EMBL; AB008264; BAB09190.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94880.1; -; Genomic_DNA.
DR EMBL; AY050909; AAK93586.1; -; mRNA.
DR EMBL; AY117215; AAM51290.1; -; mRNA.
DR EMBL; AY087906; AAM65457.1; -; mRNA.
DR RefSeq; NP_199103.1; NM_123654.4.
DR AlphaFoldDB; Q9FMN0; -.
DR SMR; Q9FMN0; -.
DR STRING; 3702.AT5G42890.1; -.
DR MetOSite; Q9FMN0; -.
DR PaxDb; Q9FMN0; -.
DR PRIDE; Q9FMN0; -.
DR ProMEX; Q9FMN0; -.
DR ProteomicsDB; 191258; -.
DR EnsemblPlants; AT5G42890.1; AT5G42890.1; AT5G42890.
DR GeneID; 834300; -.
DR Gramene; AT5G42890.1; AT5G42890.1; AT5G42890.
DR KEGG; ath:AT5G42890; -.
DR Araport; AT5G42890; -.
DR TAIR; locus:2159966; AT5G42890.
DR eggNOG; KOG4170; Eukaryota.
DR HOGENOM; CLU_105945_3_1_1; -.
DR InParanoid; Q9FMN0; -.
DR OMA; KGAYEGG; -.
DR OrthoDB; 1490913at2759; -.
DR PhylomeDB; Q9FMN0; -.
DR PRO; PR:Q9FMN0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMN0; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:UniProtKB.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEP:TAIR.
DR GO; GO:0046487; P:glyoxylate metabolic process; IMP:TAIR.
DR GO; GO:0032365; P:intracellular lipid transport; IDA:UniProtKB.
DR GO; GO:0009646; P:response to absence of light; IEP:UniProtKB.
DR GO; GO:0009744; P:response to sucrose; IEP:UniProtKB.
DR GO; GO:0009845; P:seed germination; IMP:TAIR.
DR GO; GO:0048317; P:seed morphogenesis; IMP:UniProtKB.
DR Gene3D; 3.30.1050.10; -; 1.
DR InterPro; IPR039543; POX18/UbiT/NSL-TP1.
DR InterPro; IPR003033; SCP2_sterol-bd_dom.
DR InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR PANTHER; PTHR10094; PTHR10094; 1.
DR Pfam; PF02036; SCP2; 1.
DR SUPFAM; SSF55718; SSF55718; 1.
PE 2: Evidence at transcript level;
KW Lipid transport; Peroxisome; Reference proteome; Transport.
FT CHAIN 1..123
FT /note="Sterol carrier protein 2"
FT /id="PRO_0000448701"
FT DOMAIN 16..113
FT /note="SCP2"
FT /evidence="ECO:0000255"
FT MOTIF 121..123
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
SQ SEQUENCE 123 AA; 13568 MW; D5EC8346CBC11B1F CRC64;
MANTQLKSDA IMDMMKEHLS TDAGKEVTEK IGLVYQINIA PKKLGFEEVT YIVDLKKGEV
TKGKYEGGKV DATFSFKDDD FVKVATGKMN PQMAFIRGAM KIKGSLSAAQ KFTPDIFPKP
SKL
