SCP34_ARATH
ID SCP34_ARATH Reviewed; 499 AA.
AC Q0WPR4; Q3E976; Q9FMX9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Serine carboxypeptidase-like 34;
DE EC=3.4.16.-;
DE Flags: Precursor;
GN Name=SCPL34; OrderedLocusNames=At5g23210; ORFNames=MKD15.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, TISSUE SPECIFICITY, AND NOMENCLATURE.
RX PubMed=15908604; DOI=10.1104/pp.104.057950;
RA Fraser C.M., Rider L.W., Chapple C.;
RT "An expression and bioinformatics analysis of the Arabidopsis serine
RT carboxypeptidase-like gene family.";
RL Plant Physiol. 138:1136-1148(2005).
CC -!- FUNCTION: Probable carboxypeptidase. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WPR4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WPR4-2; Sequence=VSP_022850, VSP_022851, VSP_022852,
CC VSP_022853;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:15908604}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice site
CC and to an intron retention. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S10 family. {ECO:0000305}.
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DR EMBL; AB007648; BAB11176.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93133.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93136.1; -; Genomic_DNA.
DR EMBL; AK228998; BAF00885.1; -; mRNA.
DR RefSeq; NP_001078616.1; NM_001085147.2. [Q0WPR4-2]
DR RefSeq; NP_851062.2; NM_180731.3. [Q0WPR4-1]
DR AlphaFoldDB; Q0WPR4; -.
DR SMR; Q0WPR4; -.
DR BioGRID; 17660; 4.
DR STRING; 3702.AT5G23210.1; -.
DR ESTHER; arath-SCPL34; Carboxypeptidase_S10.
DR MEROPS; S10.A39; -.
DR PaxDb; Q0WPR4; -.
DR PRIDE; Q0WPR4; -.
DR ProteomicsDB; 226608; -. [Q0WPR4-1]
DR EnsemblPlants; AT5G23210.1; AT5G23210.1; AT5G23210. [Q0WPR4-1]
DR EnsemblPlants; AT5G23210.4; AT5G23210.4; AT5G23210. [Q0WPR4-2]
DR GeneID; 832385; -.
DR Gramene; AT5G23210.1; AT5G23210.1; AT5G23210. [Q0WPR4-1]
DR Gramene; AT5G23210.4; AT5G23210.4; AT5G23210. [Q0WPR4-2]
DR KEGG; ath:AT5G23210; -.
DR Araport; AT5G23210; -.
DR TAIR; locus:2166870; AT5G23210.
DR eggNOG; KOG1282; Eukaryota.
DR HOGENOM; CLU_008523_13_0_1; -.
DR InParanoid; Q0WPR4; -.
DR OrthoDB; 625787at2759; -.
DR PhylomeDB; Q0WPR4; -.
DR PRO; PR:Q0WPR4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0WPR4; baseline and differential.
DR Genevisible; Q0WPR4; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0004185; F:serine-type carboxypeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001563; Peptidase_S10.
DR InterPro; IPR033124; Ser_caboxypep_his_AS.
DR InterPro; IPR018202; Ser_caboxypep_ser_AS.
DR PANTHER; PTHR11802; PTHR11802; 1.
DR Pfam; PF00450; Peptidase_S10; 1.
DR PRINTS; PR00724; CRBOXYPTASEC.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00560; CARBOXYPEPT_SER_HIS; 1.
DR PROSITE; PS00131; CARBOXYPEPT_SER_SER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carboxypeptidase; Disulfide bond; Glycoprotein;
KW Hydrolase; Protease; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..499
FT /note="Serine carboxypeptidase-like 34"
FT /id="PRO_0000274649"
FT ACT_SITE 200
FT /evidence="ECO:0000250"
FT ACT_SITE 419
FT /evidence="ECO:0000250"
FT ACT_SITE 471
FT /evidence="ECO:0000250"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 310
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 106..383
FT /evidence="ECO:0000250"
FT DISULFID 269..280
FT /evidence="ECO:0000250"
FT DISULFID 304..351
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..96
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_022850"
FT VAR_SEQ 97..103
FT /note="LLWLNGG -> MSKSMKR (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_022851"
FT VAR_SEQ 452..459
FT /note="GGWTVEYD -> NLVPSYKL (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_022852"
FT VAR_SEQ 460..499
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_022853"
SQ SEQUENCE 499 AA; 56329 MW; 6150FB81A2E7BCBD CRC64;
MGSHSVEFSV LVLFLVSFLL GSTSAEKLCS DNDGDNGCFR SRVLAAQRAD RVKELPGQPP
VKFRQYAGYV TVNETHGRAL FYWFFEATQN PSKKPVLLWL NGGPGCSSIG FGAAEELGPF
FPQNSSQPKL KLNPYSWNKA ANLLFLESPV GVGFSYTNTS RDIKQLGDTV TARDSYNFLV
NWFKRFPQYK SHDFYIAGES YAGHYVPQLS ELIYKENKIA SKKDFINLKG LMIGNALLDD
ETDQKGMIEY AWDHAVISDA LYEKVNKNCD FKQKLVTKEC NDALDEYFDV YKILDMYSLY
APKCVPTSTN SSTSHSVAGN RPLPAFRSIL RPRLISHNEG WRRMAAGYDP CASEYTEKYM
NRKDVQEALH ANVTNISYPW THCSDTVSFW SDAPASMLPT LRTLVSAGLR VWVFSGDTDG
RIPVTATRYS LKKLGLKIVQ DWTPWYTKLQ VGGWTVEYDG LMFVTVRGAG HQVPTFKPRE
ALQLIHHFLG NKKLPTFPF
