SCP50_ARATH
ID SCP50_ARATH Reviewed; 444 AA.
AC Q9M9Q6;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Serine carboxypeptidase-like 50;
DE EC=3.4.16.-;
DE Flags: Precursor;
GN Name=SCPL50; OrderedLocusNames=At1g15000; ORFNames=T15D22.4, T15D22.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, TISSUE SPECIFICITY, AND NOMENCLATURE.
RX PubMed=15908604; DOI=10.1104/pp.104.057950;
RA Fraser C.M., Rider L.W., Chapple C.;
RT "An expression and bioinformatics analysis of the Arabidopsis serine
RT carboxypeptidase-like gene family.";
RL Plant Physiol. 138:1136-1148(2005).
CC -!- FUNCTION: Probable carboxypeptidase. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:15908604}.
CC -!- SIMILARITY: Belongs to the peptidase S10 family. {ECO:0000305}.
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DR EMBL; AC012189; AAF31022.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29252.1; -; Genomic_DNA.
DR EMBL; AY075612; AAL91626.1; -; mRNA.
DR EMBL; BT002620; AAO11536.1; -; mRNA.
DR PIR; D86283; D86283.
DR RefSeq; NP_172953.1; NM_101369.2.
DR AlphaFoldDB; Q9M9Q6; -.
DR SMR; Q9M9Q6; -.
DR STRING; 3702.AT1G15000.1; -.
DR ESTHER; arath-SCP50; Carboxypeptidase_S10.
DR MEROPS; S10.003; -.
DR PaxDb; Q9M9Q6; -.
DR PRIDE; Q9M9Q6; -.
DR ProteomicsDB; 226606; -.
DR EnsemblPlants; AT1G15000.1; AT1G15000.1; AT1G15000.
DR GeneID; 838065; -.
DR Gramene; AT1G15000.1; AT1G15000.1; AT1G15000.
DR KEGG; ath:AT1G15000; -.
DR Araport; AT1G15000; -.
DR TAIR; locus:2196199; AT1G15000.
DR eggNOG; KOG1282; Eukaryota.
DR HOGENOM; CLU_008523_10_1_1; -.
DR OMA; PFHDLDK; -.
DR OrthoDB; 625787at2759; -.
DR PhylomeDB; Q9M9Q6; -.
DR PRO; PR:Q9M9Q6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9Q6; differential.
DR Genevisible; Q9M9Q6; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0004185; F:serine-type carboxypeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001563; Peptidase_S10.
DR InterPro; IPR033124; Ser_caboxypep_his_AS.
DR PANTHER; PTHR11802; PTHR11802; 1.
DR Pfam; PF00450; Peptidase_S10; 1.
DR PRINTS; PR00724; CRBOXYPTASEC.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00560; CARBOXYPEPT_SER_HIS; 1.
PE 2: Evidence at transcript level;
KW Carboxypeptidase; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..444
FT /note="Serine carboxypeptidase-like 50"
FT /id="PRO_0000274664"
FT ACT_SITE 170
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10075"
FT ACT_SITE 345
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10075"
FT ACT_SITE 403
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10075"
FT CARBOHYD 263
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 361
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 79..308
FT /evidence="ECO:0000250"
SQ SEQUENCE 444 AA; 49222 MW; E9E769B30DB492B8 CRC64;
MEQATTLFIL LSTLLLAVSV ESPQPPLFPD EALPTKSGYL PVKPAPGSSM FYAFYEAQEP
TTPLPDTPLL VWLQGGPGCS SMIGNFYELG PWRVVSRATD LERNPGAWNR LFGLLFVDNP
IGVGFSIAAS QQDIPTNQRQ VAEHLYAALV EFLEQNPSFE NRPVYFTGES YAGKYVPAIG
YYILKEKPNG KVNLKGLAIG NGLTDPVTQV QTHAVNVYYS GLVNAKQRVE LQKAQEISVA
LVKSQKWREA ADARTELLTL LSNMTGLATL YNTARAIPYR TDLVVDLLNQ REAKRVLGVS
ETVRFEECSD EVEDVLRADV MKSVKFMVEY ALERTQVLLY QGMLDLRDGV VSTEEWMKTM
NWSGLGMFST AERRVWKDED GVVAGYVQRW GNLCHVAVTG AGHFVPTDKA VNSRDMIEGW
VLGKGLFGGK DVKQTTSSSL YHSI
