SCPDL_HUMAN
ID SCPDL_HUMAN Reviewed; 429 AA.
AC Q8NBX0; Q8TAR0; Q9Y363;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Saccharopine dehydrogenase-like oxidoreductase;
DE EC=1.-.-.-;
GN Name=SCCPDH; ORFNames=CGI-49;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 2-29; 63-103; 141-167; 199-211; 215-228; 243-257;
RP 330-353; 366-394 AND 417-429, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION
RP AT ALA-2, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Ovarian carcinoma;
RA Bienvenut W.V.;
RL Submitted (JAN-2010) to UniProtKB.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- SIMILARITY: Belongs to the saccharopine dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; AF151807; AAD34044.1; -; mRNA.
DR EMBL; AK075178; BAC11453.1; -; mRNA.
DR EMBL; AL591848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC026185; AAH26185.1; -; mRNA.
DR CCDS; CCDS31084.1; -.
DR RefSeq; NP_057086.2; NM_016002.2.
DR AlphaFoldDB; Q8NBX0; -.
DR BioGRID; 119286; 172.
DR IntAct; Q8NBX0; 49.
DR MINT; Q8NBX0; -.
DR STRING; 9606.ENSP00000355467; -.
DR iPTMnet; Q8NBX0; -.
DR PhosphoSitePlus; Q8NBX0; -.
DR SwissPalm; Q8NBX0; -.
DR BioMuta; SCCPDH; -.
DR DMDM; 73919294; -.
DR EPD; Q8NBX0; -.
DR jPOST; Q8NBX0; -.
DR MassIVE; Q8NBX0; -.
DR MaxQB; Q8NBX0; -.
DR PaxDb; Q8NBX0; -.
DR PeptideAtlas; Q8NBX0; -.
DR PRIDE; Q8NBX0; -.
DR ProteomicsDB; 72832; -.
DR TopDownProteomics; Q8NBX0; -.
DR Antibodypedia; 34722; 131 antibodies from 25 providers.
DR DNASU; 51097; -.
DR Ensembl; ENST00000366510.4; ENSP00000355467.3; ENSG00000143653.10.
DR GeneID; 51097; -.
DR KEGG; hsa:51097; -.
DR MANE-Select; ENST00000366510.4; ENSP00000355467.3; NM_016002.3; NP_057086.2.
DR UCSC; uc001ibr.4; human.
DR CTD; 51097; -.
DR GeneCards; SCCPDH; -.
DR HGNC; HGNC:24275; SCCPDH.
DR HPA; ENSG00000143653; Low tissue specificity.
DR neXtProt; NX_Q8NBX0; -.
DR OpenTargets; ENSG00000143653; -.
DR PharmGKB; PA142670949; -.
DR VEuPathDB; HostDB:ENSG00000143653; -.
DR eggNOG; KOG2733; Eukaryota.
DR GeneTree; ENSGT00390000004799; -.
DR HOGENOM; CLU_031002_1_0_1; -.
DR InParanoid; Q8NBX0; -.
DR OMA; KQIGHKP; -.
DR OrthoDB; 887497at2759; -.
DR PhylomeDB; Q8NBX0; -.
DR TreeFam; TF314904; -.
DR PathwayCommons; Q8NBX0; -.
DR Reactome; R-HSA-114608; Platelet degranulation.
DR SignaLink; Q8NBX0; -.
DR BioGRID-ORCS; 51097; 8 hits in 1085 CRISPR screens.
DR ChiTaRS; SCCPDH; human.
DR GenomeRNAi; 51097; -.
DR Pharos; Q8NBX0; Tbio.
DR PRO; PR:Q8NBX0; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q8NBX0; protein.
DR Bgee; ENSG00000143653; Expressed in left testis and 195 other tissues.
DR ExpressionAtlas; Q8NBX0; baseline and differential.
DR Genevisible; Q8NBX0; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005811; C:lipid droplet; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0031093; C:platelet alpha granule lumen; TAS:Reactome.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0009247; P:glycolipid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032095; Sacchrp_dh-like_C.
DR InterPro; IPR005097; Sacchrp_dh_NADP.
DR Pfam; PF16653; Sacchrp_dh_C; 1.
DR Pfam; PF03435; Sacchrp_dh_NADP; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Acetylation; Direct protein sequencing; Oxidoreductase; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.5"
FT CHAIN 2..429
FT /note="Saccharopine dehydrogenase-like oxidoreductase"
FT /id="PRO_0000212840"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.5"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 418
FT /note="G -> R (in dbSNP:rs7779)"
FT /id="VAR_034486"
FT CONFLICT 37..43
FT /note="SSRLPWA -> TQPALG (in Ref. 1; AAD34044)"
FT /evidence="ECO:0000305"
FT CONFLICT 187
FT /note="P -> H (in Ref. 4; AAH26185)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="Q -> R (in Ref. 4; AAH26185)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 429 AA; 47151 MW; A93EBEDDF128A548 CRC64;
MATEQRPFHL VVFGASGFTG QFVTEEVARE QVDPERSSRL PWAVAGRSRE KLQRVLEKAA
LKLGRPTLSS EVGIIICDIA NPASLDEMAK QATVVLNCVG PYRFYGEPVI KACIENGASC
IDISGEPQFL ELMQLKYHEK AADKGVYIIG SSGFDSIPAD LGVIYTRNKM NGTLTAVESF
LTIHSGPEGL SIHDGTWKSA IYGFGDQSNL RKLRNVSNLK PVPLIGPKLK RRWPISYCRE
LKGYSIPFMG SDVSVVRRTQ RYLYENLEES PVQYAAYVTV GGITSVIKLM FAGLFFLFFV
RFGIGRQLLI KFPWFFSFGY FSKQGPTQKQ IDAASFTLTF FGQGYSQGTG TDKNKPNIKI
CTQVKGPEAG YVATPIAMVQ AAMTLLSDAS HLPKAGGVFT PGAAFSKTKL IDRLNKHGIE
FSVISSSEV
