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BETI_ECOLI
ID   BETI_ECOLI              Reviewed;         195 AA.
AC   P17446; Q2MCB0;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 157.
DE   RecName: Full=HTH-type transcriptional regulator BetI {ECO:0000255|HAMAP-Rule:MF_00768};
GN   Name=betI {ECO:0000255|HAMAP-Rule:MF_00768};
GN   OrderedLocusNames=b0313, JW0305;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1956285; DOI=10.1111/j.1365-2958.1991.tb01877.x;
RA   Lamark T., Kaasen E., Eshoo M.W., Falkenberg P., McDougall J., Strom A.R.;
RT   "DNA sequence and analysis of the bet genes encoding the osmoregulatory
RT   choline-glycine betaine pathway of Escherichia coli.";
RL   Mol. Microbiol. 5:1049-1064(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=8626294; DOI=10.1128/jb.178.6.1655-1662.1996;
RA   Lamark T., Rokenes T.P., McDougall J., Strom A.R.;
RT   "The complex bet promoters of Escherichia coli: regulation by oxygen
RT   (ArcA), choline (BetI), and osmotic stress.";
RL   J. Bacteriol. 178:1655-1662(1996).
RN   [6]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=8626295; DOI=10.1128/jb.178.6.1663-1670.1996;
RA   Rkenes T.P., Lamark T., Strom A.R.;
RT   "DNA-binding properties of the BetI repressor protein of Escherichia coli:
RT   the inducer choline stimulates BetI-DNA complex formation.";
RL   J. Bacteriol. 178:1663-1670(1996).
CC   -!- FUNCTION: Repressor involved in the biosynthesis of the osmoprotectant
CC       glycine betaine. It represses transcription of the choline transporter
CC       BetT and the genes of BetAB involved in the synthesis of glycine
CC       betaine. {ECO:0000269|PubMed:8626294, ECO:0000269|PubMed:8626295}.
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis via
CC       choline pathway [regulation].
CC   -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:8626295}.
CC   -!- DISRUPTION PHENOTYPE: In cells lacking this gene, expression of the bet
CC       promoters is stimulated by osmotic stress but not by choline.
CC       {ECO:0000269|PubMed:8626294}.
CC   -!- MISCELLANEOUS: BetI is an unusual repressor because it remains bound to
CC       DNA during induction. {ECO:0000305|PubMed:8626295}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB18039.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; X52905; CAA37091.1; -; Genomic_DNA.
DR   EMBL; U73857; AAB18039.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC73416.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76096.1; -; Genomic_DNA.
DR   PIR; S15180; S10899.
DR   RefSeq; NP_414847.3; NC_000913.3.
DR   RefSeq; WP_001335745.1; NZ_SSZK01000067.1.
DR   AlphaFoldDB; P17446; -.
DR   SMR; P17446; -.
DR   BioGRID; 4262808; 6.
DR   BioGRID; 849378; 1.
DR   IntAct; P17446; 10.
DR   STRING; 511145.b0313; -.
DR   PaxDb; P17446; -.
DR   PRIDE; P17446; -.
DR   EnsemblBacteria; AAC73416; AAC73416; b0313.
DR   EnsemblBacteria; BAE76096; BAE76096; BAE76096.
DR   GeneID; 944981; -.
DR   KEGG; ecj:JW0305; -.
DR   KEGG; eco:b0313; -.
DR   PATRIC; fig|1411691.4.peg.1964; -.
DR   EchoBASE; EB0109; -.
DR   eggNOG; COG1309; Bacteria.
DR   HOGENOM; CLU_069356_15_4_6; -.
DR   InParanoid; P17446; -.
DR   OMA; DFWSSSM; -.
DR   PhylomeDB; P17446; -.
DR   BioCyc; EcoCyc:PD00251; -.
DR   UniPathway; UPA00529; -.
DR   PRO; PR:P17446; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEP:EcoCyc.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0006970; P:response to osmotic stress; IEP:EcoCyc.
DR   HAMAP; MF_00768; HTH_type_BetI; 1.
DR   InterPro; IPR039538; BetI_C.
DR   InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   InterPro; IPR017757; Tscrpt_rep_BetI.
DR   Pfam; PF13977; TetR_C_6; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   TIGRFAMs; TIGR03384; betaine_BetI; 1.
DR   PROSITE; PS01081; HTH_TETR_1; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..195
FT                   /note="HTH-type transcriptional regulator BetI"
FT                   /id="PRO_0000070580"
FT   DOMAIN          8..68
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00768"
FT   DNA_BIND        31..50
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00768"
SQ   SEQUENCE   195 AA;  21815 MW;  B52A77B3A605E354 CRC64;
     MPKLGMQSIR RRQLIDATLE AINEVGMHDA TIAQIARRAG VSTGIISHYF RDKNGLLEAT
     MRDITSQLRD AVLNRLHALP QGSAEQRLQA IVGGNFDETQ VSSAAMKAWL AFWASSMHQP
     MLYRLQQVSS RRLLSNLVSE FRRELPREQA QEAGYGLAAL IDGLWLRAAL SGKPLDKTRA
     NSLTRHFITQ HLPTD
 
 
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