SCRA_ARATH
ID SCRA_ARATH Reviewed; 62 AA.
AC P0CG07;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Defensin-like protein A;
DE Short=AtSCRA;
DE AltName: Full=S locus cysteine-rich-like protein A;
DE AltName: Full=S locus protein 11;
DE Flags: Precursor;
GN Name=SCRA; Synonyms=SCR1, SP11; OrderedLocusNames=At4g21364;
GN ORFNames=T6K22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND MUTAGENESIS OF
RP 56-ASP--ALA-62.
RC STRAIN=cv. Columbia;
RX PubMed=20400945; DOI=10.1038/nature08927;
RA Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P.,
RA Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K.;
RT "Evolution of self-compatibility in Arabidopsis by a mutation in the male
RT specificity gene.";
RL Nature 464:1342-1346(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION.
RX PubMed=15505209; DOI=10.1073/pnas.0406970101;
RA Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.;
RT "Natural variation in expression of self-incompatibility in Arabidopsis
RT thaliana: implications for the evolution of selfing.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004).
RN [5]
RP FUNCTION.
RX PubMed=16782760; DOI=10.1093/molbev/msl042;
RA Bechsgaard J.S., Castric V., Charlesworth D., Vekemans X., Schierup M.H.;
RT "The transition to self-compatibility in Arabidopsis thaliana and evolution
RT within S-haplotypes over 10 Myr.";
RL Mol. Biol. Evol. 23:1741-1750(2006).
RN [6]
RP FUNCTION.
RX PubMed=19300485; DOI=10.1371/journal.pgen.1000426;
RA Boggs N.A., Nasrallah J.B., Nasrallah M.E.;
RT "Independent S-locus mutations caused self-fertility in Arabidopsis
RT thaliana.";
RL PLoS Genet. 5:E1000426-E1000426(2009).
CC -!- FUNCTION: Truncated and inactivated form of SCRA, a protein involved in
CC male-mediated self-incompatibility when active. Most A.thaliana
CC cultivars contain such an inactive form and thus, are self-fertiles.
CC {ECO:0000269|PubMed:15505209, ECO:0000269|PubMed:16782760,
CC ECO:0000269|PubMed:19300485, ECO:0000269|PubMed:20400945}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- MISCELLANEOUS: By contrast to A.lyrata and A.halleri, self-incompatible
CC species with full-length SCRA isoforms, A.thaliana is self-compatible
CC with impaired SCRA protein.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Do it yourself - Issue 128
CC of May 2011;
CC URL="https://web.expasy.org/spotlight/back_issues/128";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL031187; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; P0CG07; -.
DR PRIDE; P0CG07; -.
DR Araport; AT4G21364; -.
DR PRO; PR:P0CG07; -.
DR Proteomes; UP000006548; Chromosome 4.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0060320; P:rejection of self pollen; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR InterPro; IPR010682; SCRL.
DR PANTHER; PTHR34450; PTHR34450; 1.
DR Pfam; PF06876; SCRL; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Reference proteome; Secreted; Self-incompatibility; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..62
FT /note="Defensin-like protein A"
FT /id="PRO_0000394667"
FT DISULFID 42..52
FT /evidence="ECO:0000250"
FT MUTAGEN 56..62
FT /note="DAKKTLA->IAKNFRPSRPFECNCQTFDKGGICYCKKCLV: Confers
FT male-driven self-incompatibility."
FT /evidence="ECO:0000269|PubMed:20400945"
SQ SEQUENCE 62 AA; 7226 MW; 863A76970BCF46A9 CRC64;
MRCVVLFMVS CLLIVLLINH FEEVEAQKWN KCFLRDIFPG KCEHDANAKL RCKEDDAKKT
LA
