SCRB2_RAT
ID SCRB2_RAT Reviewed; 478 AA.
AC P27615;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Lysosome membrane protein 2;
DE AltName: Full=85 kDa lysosomal membrane sialoglycoprotein;
DE Short=LGP85;
DE AltName: Full=CD36 antigen-like 2;
DE AltName: Full=Lysosome membrane protein II;
DE Short=LIMP II;
DE AltName: Full=Scavenger receptor class B member 2;
DE AltName: CD_antigen=CD36;
GN Name=Scarb2; Synonyms=Cd36l2, Limpii;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-13 AND 41-63, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Liver;
RX PubMed=1715871; DOI=10.1016/s0021-9258(18)55375-8;
RA Vega M.A., Segui-Real B., Alcalde Garcia J., Cales C., Rodriquez F.,
RA Vanderkerckhove J., Sandoval I.V.;
RT "Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a
RT novel 74-kDa lysosomal membrane protein related to the surface adhesion
RT protein CD36.";
RL J. Biol. Chem. 266:16818-16824(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Liver;
RX PubMed=1859403; DOI=10.1016/0006-291x(91)90127-s;
RA Fujita H., Ezaki J., Noguchi Y., Kono A., Himeno M., Kato K.;
RT "Isolation and sequencing of a cDNA clone encoding 85kDa sialoglycoprotein
RT in rat liver lysosomal membranes.";
RL Biochem. Biophys. Res. Commun. 178:444-452(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 32-48; 116-121; 135-161; 228-234; 263-294; 331-348;
RP 361-378; 382-402 AND 460-472, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RA Lubec G., Kang S.U., Lubec S.;
RL Submitted (SEP-2007) to UniProtKB.
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-122 AND ASN-412, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24090084; DOI=10.1021/pr400783j;
RA Parker B.L., Thaysen-Andersen M., Solis N., Scott N.E., Larsen M.R.,
RA Graham M.E., Packer N.H., Cordwell S.J.;
RT "Site-specific glycan-peptide analysis for determination of N-glycoproteome
RT heterogeneity.";
RL J. Proteome Res. 12:5791-5800(2013).
CC -!- FUNCTION: Acts as a lysosomal receptor for glucosylceramidase (GBA)
CC targeting. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GBA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:1715871,
CC ECO:0000269|PubMed:1859403}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:1715871, ECO:0000269|PubMed:1859403}.
CC -!- PTM: Acylated by palmitic acid group(s).
CC -!- SIMILARITY: Belongs to the CD36 family. {ECO:0000305}.
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DR EMBL; M68965; AAA41531.1; -; mRNA.
DR EMBL; D10587; BAA01444.1; -; mRNA.
DR EMBL; BC061853; AAH61853.1; -; mRNA.
DR PIR; JH0241; JH0241.
DR RefSeq; NP_446453.1; NM_054001.2.
DR AlphaFoldDB; P27615; -.
DR SMR; P27615; -.
DR IntAct; P27615; 3.
DR STRING; 10116.ENSRNOP00000003052; -.
DR GlyGen; P27615; 11 sites, 6 N-linked glycans (2 sites).
DR iPTMnet; P27615; -.
DR SwissPalm; P27615; -.
DR jPOST; P27615; -.
DR PaxDb; P27615; -.
DR PRIDE; P27615; -.
DR Ensembl; ENSRNOT00000103647; ENSRNOP00000097524; ENSRNOG00000002225.
DR GeneID; 117106; -.
DR KEGG; rno:117106; -.
DR UCSC; RGD:621882; rat.
DR CTD; 950; -.
DR RGD; 621882; Scarb2.
DR eggNOG; KOG3776; Eukaryota.
DR GeneTree; ENSGT00940000153372; -.
DR HOGENOM; CLU_019853_3_0_1; -.
DR InParanoid; P27615; -.
DR OMA; DVFGMRP; -.
DR OrthoDB; 1106566at2759; -.
DR PhylomeDB; P27615; -.
DR TreeFam; TF317925; -.
DR Reactome; R-RNO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-RNO-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:P27615; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000002225; Expressed in lung and 19 other tissues.
DR Genevisible; P27615; RN.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030666; C:endocytic vesicle membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043202; C:lysosomal lumen; ISO:RGD.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0038024; F:cargo receptor activity; ISO:RGD.
DR GO; GO:0051087; F:chaperone binding; ISO:RGD.
DR GO; GO:0015485; F:cholesterol binding; ISO:RGD.
DR GO; GO:0019899; F:enzyme binding; ISO:RGD.
DR GO; GO:0031210; F:phosphatidylcholine binding; ISO:RGD.
DR GO; GO:0001786; F:phosphatidylserine binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0005044; F:scavenger receptor activity; ISO:RGD.
DR GO; GO:0015917; P:aminophospholipid transport; ISO:RGD.
DR GO; GO:0010467; P:gene expression; ISO:RGD.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IDA:ParkinsonsUK-UCL.
DR GO; GO:0006622; P:protein targeting to lysosome; ISO:RGD.
DR GO; GO:0006898; P:receptor-mediated endocytosis; ISO:RGD.
DR GO; GO:0043471; P:regulation of cellular carbohydrate catabolic process; ISO:RGD.
DR GO; GO:1905123; P:regulation of glucosylceramidase activity; ISO:RGD.
DR GO; GO:0007605; P:sensory perception of sound; ISO:RGD.
DR InterPro; IPR002159; CD36_fam.
DR InterPro; IPR005429; LimpII.
DR PANTHER; PTHR11923; PTHR11923; 1.
DR Pfam; PF01130; CD36; 1.
DR PRINTS; PR01609; CD36FAMILY.
DR PRINTS; PR01611; LIMPII.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; Lipoprotein;
KW Lysosome; Membrane; Palmitate; Receptor; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1715871"
FT CHAIN 2..478
FT /note="Lysosome membrane protein 2"
FT /id="PRO_0000144157"
FT TOPO_DOM 2..4
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..433
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..478
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 155..191
FT /note="Important for interaction with GBA"
FT /evidence="ECO:0000250"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 105
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0007744|PubMed:24090084"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 224
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 249
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 304
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 325
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0007744|PubMed:24090084"
FT CARBOHYD 430
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 274..329
FT /evidence="ECO:0000250"
FT DISULFID 312..318
FT /evidence="ECO:0000250"
SQ SEQUENCE 478 AA; 54091 MW; 3EA6E653C38E3002 CRC64;
MARCCFYTAG TLSLLLLVTS VTLLVARVFQ KAVDQTIEKN MVLQNGTKVF DSWEKPPLPV
YIQFYFFNVT NPEEILQGEI PLLEEVGPYT YRELRNKANV QFGENGTTIS AVTNKAYIFE
RNQSVGDPTV DLIRTINIPL LTVVEMAQQP FLREIIEAML KAYQQTLFVT HTVHELLWGY
KDEVLSLVHI FRPDVSPNFG LFYERNGTND GEYVFLTGED NYLNFTKIVE WNGKTSLDWW
TTDTCNMING TDGDSFHPLI SKDETLYIFP SDFCRSVYIT FSSFENVEGL PAFRYKVPAE
ILANSSENAG FCIPEGNCMD AGVLNVSICK NGAPIIMSFP HFYQADEKFV SAIKGMRPNK
EEHESFVDIN PLTGIILRGA KRFQINTYVK KLDDFVETGN IRTMVFPVMY LNESVLIDKE
TASQLKSVIN TTLIVTNIPY IIMALGVFFG LIFTWLACRG QGSTDEGTAD ERAPLIRT
