ID   SCRB_FUSMR              Reviewed;          33 AA.
AC   Q09122;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Sucrose-6-phosphate hydrolase;
DE            Short=Sucrase;
DE            EC=3.2.1.26;
DE   AltName: Full=Invertase;
DE   Flags: Fragment;
OS   Fusobacterium mortiferum.
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=850;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES,
RP   PATHWAY, AND INDUCTION.
RC   STRAIN=ATCC 25557 / DSM 19809 / CCUG 14475 / VPI 4123A;
RX   PubMed=1533618; DOI=10.1128/jb.174.10.3227-3235.1992;
RA   Thompson J., Nguyen N.Y., Robrish S.A.;
RT   "Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport,
RT   catabolism, and products.";
RL   J. Bacteriol. 174:3227-3235(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10067,
CC         ECO:0000269|PubMed:1533618};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=40 mM for sucrose {ECO:0000269|PubMed:1533618};
CC         KM=0.28 mM for sucrose-6-phosphate {ECO:0000269|PubMed:1533618};
CC         Vmax=43 umol/min/mg enzyme for sucrose {ECO:0000269|PubMed:1533618};
CC         Vmax=270 umol/min/mg enzyme for sucrose 6-phosphate
CC         {ECO:0000269|PubMed:1533618};
CC       pH dependence:
CC         Optimum pH is 7.0. {ECO:0000269|PubMed:1533618};
CC   -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC       {ECO:0000269|PubMed:1533618}.
CC   -!- INDUCTION: Induced by sucrose. {ECO:0000269|PubMed:1533618}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC       {ECO:0000269|PubMed:1533618}.
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DR   SABIO-RK; Q09122; -.
DR   UniPathway; UPA00238; -.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IDA:UniProtKB.
DR   GO; GO:0044275; P:cellular carbohydrate catabolic process; IDA:UniProtKB.
DR   GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Direct protein sequencing; Glycosidase; Hydrolase.
FT   CHAIN           1..>33
FT                   /note="Sucrose-6-phosphate hydrolase"
FT                   /id="PRO_0000169871"
FT   ACT_SITE        18
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT   BINDING         15..18
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   NON_TER         33
FT                   /evidence="ECO:0000303|PubMed:1533618"
SQ   SEQUENCE   33 AA;  4143 MW;  54C9FAF53856B597 CRC64;
     MDKKEYINRL NEQKPLQEMX SRDRYLXNFH LIP