ID   SCRB_VIBC3              Reviewed;         546 AA.
AC   A5EZZ8; C3M5S8;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Probable sucrose-6-phosphate hydrolase;
DE            Short=Sucrase;
DE            EC=3.2.1.26;
DE   AltName: Full=Invertase;
GN   Name=cscA; OrderedLocusNames=VC0395_0599, VC395_A0657;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Enables the bacterium to metabolize sucrose as a sole carbon
CC       source. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC   -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
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DR   EMBL; CP000626; ABQ18842.1; -; Genomic_DNA.
DR   EMBL; CP001236; ACP11491.1; -; Genomic_DNA.
DR   RefSeq; WP_000923294.1; NZ_JAACZH010000013.1.
DR   AlphaFoldDB; A5EZZ8; -.
DR   SMR; A5EZZ8; -.
DR   STRING; 345073.VC395_A0657; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   EnsemblBacteria; ABQ18842; ABQ18842; VC0395_0599.
DR   KEGG; vco:VC0395_0599; -.
DR   KEGG; vcr:VC395_A0657; -.
DR   PATRIC; fig|345073.21.peg.3393; -.
DR   eggNOG; COG1621; Bacteria.
DR   HOGENOM; CLU_001528_7_1_6; -.
DR   OMA; WMGVPDG; -.
DR   UniPathway; UPA00238; -.
DR   Proteomes; UP000000249; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR018053; Glyco_hydro_32_AS.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   InterPro; IPR006232; Suc6P_hydrolase.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
DR   TIGRFAMs; TIGR01322; scrB_fam; 1.
DR   PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycosidase; Hydrolase.
FT   CHAIN           1..546
FT                   /note="Probable sucrose-6-phosphate hydrolase"
FT                   /id="PRO_0000341299"
FT   ACT_SITE        108
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT   BINDING         105..108
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         124
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         167..168
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         228..229
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         283
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   546 AA;  62312 MW;  8F7360DCCEC6B2CC CRC64;
     MLLDTLLELA GGINNVTRIL APQGQVVLAL KHPPHAPHLP DDVSLQSVLG EWQLSVQRTA
     EVSDQQLAAI GKAIAERQKR ATLPYQTALD CPYRPQWHIS PPQGLLNDPN GFIYHQEEYH
     LFYQWHPFAC EHKDKYWVHL KSLDLVHWQW QSVALTPSDW FDSHGVFSGH AVSHQQDLWL
     FYTGNTRLGT ERQRQTMQCA ARMNANGEFE KLGPVIRCLP EGVTEHIRDP KVIYTQGKWQ
     MLLGAQTLAH QGRLAVYHSD DLLHWHFDKL YGDELGDYGY MWECPDWFEL QGEAFFVFGP
     QGIASANPHH TIEHQNRIFR ATQNAQGEIA LLQGWPLDEG FDFYAPQTAQ TADGRRVLCG
     WMGLPDETQH PSCDQGWIHQ LTALRELEWR EGKIYQHPLR ELDTLQSEPH TLLLSDTVTE
     LKTKSFDLQV TLPWGCELRL MQNAQYCVTL TLDAENRLLR LDRSATQIRQ GDTIRELKLD
     SPTVELRILA DQSSLEIFIN QGEHVMTSRI FTPLDATGIS LHGASVDAKL YYMAPASAPF
     NLEVNV