SCRIB_DROME
ID SCRIB_DROME Reviewed; 1851 AA.
AC Q7KRY7; A0A0B4K7L2; A4V3G5; Q59DT7; Q59DT8; Q6AWK8; Q7K9X1; Q7KA36; Q86QS7;
AC Q86QS8; Q86QS9; Q86QT0; Q8IMR8; Q8IMR9; Q8T003; Q95SV4; Q9VBE4;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Protein lap4;
DE AltName: Full=Protein scribble;
DE AltName: Full=Protein smell-impaired;
GN Name=scrib {ECO:0000312|EMBL:AAF56598.2};
GN Synonyms=smi {ECO:0000303|PubMed:12930751},
GN vart {ECO:0000312|EMBL:CAB71137.1}; ORFNames=CG5462;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAF26357.2}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo {ECO:0000269|PubMed:10688207};
RX PubMed=10688207; DOI=10.1038/35001108;
RA Bilder D., Perrimon N.;
RT "Localization of apical epithelial determinants by the basolateral PDZ
RT protein Scribble.";
RL Nature 403:676-680(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAB70601.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), FUNCTION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11578873; DOI=10.1016/s0925-4773(01)00482-8;
RA Li M., Marhold J., Gatos A., Torok I., Mechler B.M.;
RT "Differential expression of two scribble isoforms during Drosophila
RT embryogenesis.";
RL Mech. Dev. 108:185-190(2001).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAO32792.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; C; D; E; G AND J), NUCLEOTIDE
RP SEQUENCE [MRNA] OF 639-1851 (ISOFORM F), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Head {ECO:0000269|PubMed:12930751};
RX PubMed=12930751; DOI=10.1093/genetics/164.4.1447;
RA Ganguly I., Mackay T.F.C., Anholt R.R.H.;
RT "Scribble is essential for olfactory behavior in Drosophila melanogaster.";
RL Genetics 164:1447-1457(2003).
RN [4] {ECO:0000312|EMBL:AAF56598.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5] {ECO:0000305, ECO:0000312|EMBL:AAF56598.2}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6] {ECO:0000305, ECO:0000312|EMBL:AAL39806.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL39806.2}; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000305, ECO:0000312|EMBL:AAL25508.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1647-1851 (ISOFORMS A/C/D/E/G/H).
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [8] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=10884224; DOI=10.1126/science.289.5476.113;
RA Bilder D., Li M., Perrimon N.;
RT "Cooperative regulation of cell polarity and growth by Drosophila tumor
RT suppressors.";
RL Science 289:113-116(2000).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-433; SER-435; SER-700;
RP SER-702; SER-705; SER-834; SER-837; SER-1031; SER-1041; SER-1475; SER-1477;
RP SER-1478 AND THR-1599, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for polarization of the embryonic, imaginal disk and
CC follicular epithelia. Specifically restricts apical membrane
CC determinants to the apical cell surface; acts to exclude crb from the
CC basolateral domain and define adherens junction position. Regulates
CC cellular growth and differentiation; acts as a tumor suppressor.
CC Essential for odor guided behavior. {ECO:0000269|PubMed:10688207,
CC ECO:0000269|PubMed:10884224, ECO:0000269|PubMed:11578873,
CC ECO:0000269|PubMed:12930751}.
CC -!- INTERACTION:
CC Q7KRY7; Q4AB30: gukh; NbExp=2; IntAct=EBI-419788, EBI-8282973;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane
CC protein. Cell junction, tight junction. Note=Localized to epithelial
CC septate junction at the boundary of the apical and basolateral cell
CC surfaces.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=11;
CC Name=D {ECO:0000269|PubMed:12930751};
CC IsoId=Q7KRY7-1; Sequence=Displayed;
CC Name=A {ECO:0000269|PubMed:11578873}; Synonyms=B
CC {ECO:0000303|PubMed:10731132}, Scrib1 {ECO:0000303|PubMed:11578873},
CC Vartul-1 {ECO:0000312|EMBL:CAB70601.1};
CC IsoId=Q7KRY7-2; Sequence=VSP_050879;
CC Name=C {ECO:0000269|PubMed:11578873}; Synonyms=Scrib2
CC {ECO:0000303|PubMed:11578873}, Vartul-2 {ECO:0000312|EMBL:CAB71137.1};
CC IsoId=Q7KRY7-3; Sequence=VSP_050880, VSP_050881;
CC Name=E {ECO:0000269|PubMed:12930751};
CC IsoId=Q7KRY7-4; Sequence=VSP_050877, VSP_050878, VSP_018595;
CC Name=F {ECO:0000269|PubMed:12930751};
CC IsoId=Q7KRY7-5; Sequence=VSP_050882, VSP_050883, VSP_050884;
CC Name=G {ECO:0000269|PubMed:12930751};
CC IsoId=Q7KRY7-6; Sequence=VSP_050876, VSP_050878, VSP_050882;
CC Name=H;
CC IsoId=Q7KRY7-7; Sequence=VSP_035210, VSP_018595;
CC Name=I;
CC IsoId=Q7KRY7-8; Sequence=VSP_050883, VSP_050884;
CC Name=J;
CC IsoId=Q7KRY7-9; Sequence=VSP_018595;
CC Name=K;
CC IsoId=Q7KRY7-10; Sequence=VSP_050879, VSP_042867;
CC Name=P {ECO:0000312|FlyBase:FBgn0263289};
CC IsoId=Q7KRY7-11; Sequence=VSP_058487, VSP_058488, VSP_058489;
CC -!- TISSUE SPECIFICITY: During germ band extension, expression of isoform A
CC occurs predominantly in neuroblasts derived from the neuro-ectoderm and
CC later is restricted to CNS neurons and pole cells. Isoform C is
CC strongly expressed in PNS and a subset of CNS neurons. In the adult,
CC expressed in third antennal segment and maxillary palps, major
CC olfactory organs and in Johnstons organ in the second antennal segment.
CC Expression is also observed in cortical regions of the brain. Isoforms
CC expressed in epithelia are coexpressed with dlg1 throughout
CC development. {ECO:0000269|PubMed:10884224, ECO:0000269|PubMed:11578873,
CC ECO:0000269|PubMed:12930751}.
CC -!- DEVELOPMENTAL STAGE: Expressed both zygotically and maternally in
CC embryos. Isoform A and isoform C have high expression throughout
CC embryonic development and very low expression in later developmental
CC stages. In adults, isoform A is a male-specific isoform and isoform C a
CC female specific. {ECO:0000269|PubMed:10688207,
CC ECO:0000269|PubMed:11578873, ECO:0000269|PubMed:12930751}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit aberrant cell shape and loss of the
CC monolayer organization of embryonic epithelia creating a corrugated
CC cuticular surface that is riddled with holes hence the gene name
CC scribble. Misdistribution of apical proteins and adherens junctions to
CC the basolateral cell surface is also exhibited.
CC {ECO:0000269|PubMed:10688207}.
CC -!- SIMILARITY: Belongs to the LAP (LRR and PDZ) protein family.
CC {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL25508.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AF190774; AAF26357.2; -; mRNA.
DR EMBL; AJ252084; CAB70601.1; -; mRNA.
DR EMBL; AJ271647; CAB71137.1; -; mRNA.
DR EMBL; AY167903; AAO32791.1; -; mRNA.
DR EMBL; AY167904; AAO32792.1; -; mRNA.
DR EMBL; AY167905; AAO32793.1; -; mRNA.
DR EMBL; AY167906; AAO32794.1; -; mRNA.
DR EMBL; AE014297; AAF56598.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN14076.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14077.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN14078.1; -; Genomic_DNA.
DR EMBL; AE014297; AAX52995.2; -; Genomic_DNA.
DR EMBL; AE014297; AAX52996.1; -; Genomic_DNA.
DR EMBL; AE014297; ABI31210.3; -; Genomic_DNA.
DR EMBL; AE014297; AFH06635.1; -; Genomic_DNA.
DR EMBL; AY069661; AAL39806.2; -; mRNA.
DR EMBL; BT015240; AAT94469.1; -; mRNA.
DR EMBL; AY060469; AAL25508.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001014669.1; NM_001014669.3. [Q7KRY7-8]
DR RefSeq; NP_001014670.2; NM_001014670.4. [Q7KRY7-7]
DR RefSeq; NP_001036761.3; NM_001043296.3. [Q7KRY7-2]
DR RefSeq; NP_001247318.1; NM_001260389.1. [Q7KRY7-10]
DR RefSeq; NP_524754.2; NM_080015.4. [Q7KRY7-1]
DR RefSeq; NP_733154.1; NM_170275.3. [Q7KRY7-2]
DR RefSeq; NP_733155.2; NM_170276.3. [Q7KRY7-11]
DR RefSeq; NP_733156.1; NM_170277.4. [Q7KRY7-3]
DR PDB; 5WOU; X-ray; 1.55 A; A=726-820.
DR PDBsum; 5WOU; -.
DR AlphaFoldDB; Q7KRY7; -.
DR SMR; Q7KRY7; -.
DR BioGRID; 69066; 58.
DR IntAct; Q7KRY7; 3.
DR MINT; Q7KRY7; -.
DR STRING; 7227.FBpp0300852; -.
DR iPTMnet; Q7KRY7; -.
DR SwissPalm; Q7KRY7; -.
DR PaxDb; Q7KRY7; -.
DR PeptideAtlas; Q7KRY7; -.
DR PRIDE; Q7KRY7; -.
DR EnsemblMetazoa; FBtr0308620; FBpp0300844; FBgn0263289. [Q7KRY7-2]
DR EnsemblMetazoa; FBtr0308621; FBpp0300845; FBgn0263289. [Q7KRY7-2]
DR EnsemblMetazoa; FBtr0308622; FBpp0300846; FBgn0263289. [Q7KRY7-7]
DR EnsemblMetazoa; FBtr0308623; FBpp0300847; FBgn0263289. [Q7KRY7-8]
DR EnsemblMetazoa; FBtr0308624; FBpp0300848; FBgn0263289. [Q7KRY7-3]
DR EnsemblMetazoa; FBtr0308625; FBpp0300849; FBgn0263289. [Q7KRY7-1]
DR EnsemblMetazoa; FBtr0308627; FBpp0300851; FBgn0263289. [Q7KRY7-10]
DR EnsemblMetazoa; FBtr0308632; FBpp0300856; FBgn0263289. [Q7KRY7-11]
DR GeneID; 44448; -.
DR KEGG; dme:Dmel_CG43398; -.
DR UCSC; CG5462-RB; d. melanogaster.
DR CTD; 23513; -.
DR FlyBase; FBgn0263289; scrib.
DR VEuPathDB; VectorBase:FBgn0263289; -.
DR eggNOG; KOG0619; Eukaryota.
DR InParanoid; Q7KRY7; -.
DR PhylomeDB; Q7KRY7; -.
DR SignaLink; Q7KRY7; -.
DR BioGRID-ORCS; 44448; 0 hits in 3 CRISPR screens.
DR ChiTaRS; scrib; fly.
DR GenomeRNAi; 44448; -.
DR PRO; PR:Q7KRY7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0263289; Expressed in eye disc (Drosophila) and 48 other tissues.
DR ExpressionAtlas; Q7KRY7; baseline and differential.
DR Genevisible; Q7KRY7; DM.
DR GO; GO:0005912; C:adherens junction; IBA:GO_Central.
DR GO; GO:0016327; C:apicolateral plasma membrane; IDA:FlyBase.
DR GO; GO:1990794; C:basolateral part of cell; IDA:FlyBase.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:FlyBase.
DR GO; GO:0005923; C:bicellular tight junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005938; C:cell cortex; IDA:FlyBase.
DR GO; GO:0030054; C:cell junction; IBA:GO_Central.
DR GO; GO:0045169; C:fusome; IDA:FlyBase.
DR GO; GO:0016328; C:lateral plasma membrane; IDA:FlyBase.
DR GO; GO:0031594; C:neuromuscular junction; IDA:FlyBase.
DR GO; GO:0014069; C:postsynaptic density; IBA:GO_Central.
DR GO; GO:0005918; C:septate junction; IDA:FlyBase.
DR GO; GO:0030714; P:anterior/posterior axis specification, follicular epithelium; IMP:BHF-UCL.
DR GO; GO:0045175; P:basal protein localization; IMP:FlyBase.
DR GO; GO:0060581; P:cell fate commitment involved in pattern specification; IMP:BHF-UCL.
DR GO; GO:0001708; P:cell fate specification; IMP:BHF-UCL.
DR GO; GO:0000902; P:cell morphogenesis; IMP:FlyBase.
DR GO; GO:0098609; P:cell-cell adhesion; IBA:GO_Central.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0007391; P:dorsal closure; IMP:FlyBase.
DR GO; GO:0045198; P:establishment of epithelial cell apical/basal polarity; IMP:FlyBase.
DR GO; GO:0001737; P:establishment of imaginal disc-derived wing hair orientation; IMP:FlyBase.
DR GO; GO:0042067; P:establishment of ommatidial planar polarity; IMP:FlyBase.
DR GO; GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IMP:FlyBase.
DR GO; GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IBA:GO_Central.
DR GO; GO:0016332; P:establishment or maintenance of polarity of embryonic epithelium; TAS:FlyBase.
DR GO; GO:0016334; P:establishment or maintenance of polarity of follicular epithelium; IMP:FlyBase.
DR GO; GO:0016336; P:establishment or maintenance of polarity of larval imaginal disc epithelium; IMP:FlyBase.
DR GO; GO:0072002; P:Malpighian tubule development; IMP:FlyBase.
DR GO; GO:0007613; P:memory; IMP:FlyBase.
DR GO; GO:0001738; P:morphogenesis of a polarized epithelium; TAS:FlyBase.
DR GO; GO:0016331; P:morphogenesis of embryonic epithelium; TAS:FlyBase.
DR GO; GO:0016333; P:morphogenesis of follicular epithelium; IMP:FlyBase.
DR GO; GO:0016335; P:morphogenesis of larval imaginal disc epithelium; IMP:FlyBase.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IMP:FlyBase.
DR GO; GO:0045571; P:negative regulation of imaginal disc growth; IMP:FlyBase.
DR GO; GO:0098968; P:neurotransmitter receptor transport postsynaptic membrane to endosome; IBA:GO_Central.
DR GO; GO:0098887; P:neurotransmitter receptor transport, endosome to postsynaptic membrane; IBA:GO_Central.
DR GO; GO:0042048; P:olfactory behavior; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:BHF-UCL.
DR GO; GO:0007318; P:pole plasm protein localization; IMP:BHF-UCL.
DR GO; GO:0007464; P:R3/R4 cell fate commitment; IMP:FlyBase.
DR GO; GO:0043113; P:receptor clustering; IBA:GO_Central.
DR GO; GO:0097120; P:receptor localization to synapse; IBA:GO_Central.
DR GO; GO:0030100; P:regulation of endocytosis; IMP:FlyBase.
DR GO; GO:0050803; P:regulation of synapse structure or activity; IMP:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IEA:UniProtKB-KW.
DR GO; GO:0019991; P:septate junction assembly; IMP:FlyBase.
DR GO; GO:0048863; P:stem cell differentiation; IMP:FlyBase.
DR GO; GO:0072089; P:stem cell proliferation; IMP:FlyBase.
DR GO; GO:0007472; P:wing disc morphogenesis; IMP:FlyBase.
DR GO; GO:0045186; P:zonula adherens assembly; IMP:FlyBase.
DR Gene3D; 2.30.42.10; -; 4.
DR Gene3D; 3.80.10.10; -; 4.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR Pfam; PF13855; LRR_8; 3.
DR Pfam; PF00595; PDZ; 4.
DR SMART; SM00369; LRR_TYP; 13.
DR SMART; SM00228; PDZ; 4.
DR SUPFAM; SSF50156; SSF50156; 4.
DR PROSITE; PS51450; LRR; 15.
DR PROSITE; PS50106; PDZ; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell junction; Cell membrane;
KW Coiled coil; Cytoplasm; Developmental protein; Differentiation;
KW Leucine-rich repeat; Membrane; Neurogenesis; Olfaction; Phosphoprotein;
KW Reference proteome; Repeat; Sensory transduction; Tight junction.
FT CHAIN 1..1851
FT /note="Protein lap4"
FT /id="PRO_0000188307"
FT REPEAT 38..59
FT /note="LRR 1"
FT REPEAT 61..82
FT /note="LRR 2"
FT REPEAT 84..105
FT /note="LRR 3"
FT REPEAT 107..128
FT /note="LRR 4"
FT REPEAT 130..152
FT /note="LRR 5"
FT REPEAT 153..174
FT /note="LRR 6"
FT REPEAT 176..197
FT /note="LRR 7"
FT REPEAT 199..220
FT /note="LRR 8"
FT REPEAT 222..243
FT /note="LRR 9"
FT REPEAT 245..267
FT /note="LRR 10"
FT REPEAT 268..289
FT /note="LRR 11"
FT REPEAT 291..312
FT /note="LRR 12"
FT REPEAT 314..335
FT /note="LRR 13"
FT REPEAT 337..358
FT /note="LRR 14"
FT REPEAT 360..382
FT /note="LRR 15"
FT REPEAT 383..403
FT /note="LRR 16"
FT DOMAIN 731..818
FT /note="PDZ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 929..1019
FT /note="PDZ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 1239..1329
FT /note="PDZ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 1336..1428
FT /note="PDZ 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT REGION 427..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 584..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 656..719
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1067..1150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1448..1485
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1647..1751
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1772..1851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 471..492
FT /evidence="ECO:0000255"
FT COILED 677..693
FT /evidence="ECO:0000255"
FT COILED 1753..1790
FT /evidence="ECO:0000255"
FT COMPBIAS 432..459
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 601..623
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 656..674
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 686..712
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1067..1116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1132..1150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1448..1477
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1704..1751
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1772..1793
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1827..1851
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 433
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 435
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 700
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 702
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 705
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 834
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 837
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1031
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1041
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1475
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1477
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1478
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1599
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 97..137
FT /note="Missing (in isoform G)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050876"
FT VAR_SEQ 99..137
FT /note="Missing (in isoform E)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050877"
FT VAR_SEQ 427
FT /note="P -> PDYPPMTLYRSSEKFDSTEFFSYQQK (in isoform P)"
FT /evidence="ECO:0000305"
FT /id="VSP_058487"
FT VAR_SEQ 446..1051
FT /note="Missing (in isoform E and isoform G)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050878"
FT VAR_SEQ 716
FT /note="P -> PANQAMFNVKIKVKEGNNTLPRNGQRSNGNFSYSGAGSGRNSPAMSS
FT LRSSLASSNRSPSGSLQRKGKRVRIVTSYDPIDRDYERQRQHY (in isoform H)"
FT /evidence="ECO:0000305"
FT /id="VSP_035210"
FT VAR_SEQ 1144..1238
FT /note="Missing (in isoform A and isoform K)"
FT /evidence="ECO:0000303|PubMed:10688207,
FT ECO:0000303|PubMed:11578873, ECO:0000303|PubMed:12930751,
FT ECO:0000303|Ref.6"
FT /id="VSP_050879"
FT VAR_SEQ 1238
FT /note="Missing (in isoform E, isoform H and isoform J)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_018595"
FT VAR_SEQ 1239..1247
FT /note="EVVLPKNQG -> VTISTSRIH (in isoform C)"
FT /evidence="ECO:0000303|PubMed:11578873,
FT ECO:0000303|PubMed:12930751"
FT /id="VSP_050880"
FT VAR_SEQ 1248..1851
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:11578873,
FT ECO:0000303|PubMed:12930751"
FT /id="VSP_050881"
FT VAR_SEQ 1468..1471
FT /note="Missing (in isoform F and isoform G)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050882"
FT VAR_SEQ 1697..1711
FT /note="STPASRDGAEEQQEE -> VCTHLTVDARLLSWL (in isoform F and
FT isoform I)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050883"
FT VAR_SEQ 1697..1704
FT /note="STPASRDG -> LTAEQRIA (in isoform P)"
FT /evidence="ECO:0000305"
FT /id="VSP_058488"
FT VAR_SEQ 1705..1851
FT /note="Missing (in isoform P)"
FT /evidence="ECO:0000305"
FT /id="VSP_058489"
FT VAR_SEQ 1712..1851
FT /note="Missing (in isoform F and isoform I)"
FT /evidence="ECO:0000303|PubMed:12930751"
FT /id="VSP_050884"
FT VAR_SEQ 1839..1851
FT /note="RNPLDEIEAVFRS -> SAEKRASWRAARLRSLEQGAVEAQDVIKNMRKMTD
FT DLITHESRASEVETCKERIISLELQVPETEDEGKLDDTQPPLELEADDFQDDEDDDDDD
FT DDEDDFEDEDEEEDTASIVTVQANNEKLFLRHDSDTYGPSSIESVASGKVRIKPTPLSL
FT HQTLAKETTIVEVLNPVIGGDGSARTIHVSGGLPFDEDAFEQGAVAVLRSETFVLPGAA
FT GGGRSELSLNAKMKTVLEELLENERVKLNLQKSLEGEEGEDDDDEDENGSAYGDARDEE
FT ESAYGDAIDDVVDFGSATVTSRAANDKDDVKNGNQQLLEELIKDSNRNTIIAKLAGQLS
FT DDDEEDDDEEESSDSSDDDSDDSDEEDNAQQQLNVTYVQGAQVIENLNTLATGGKLQKS
FT QTYTAIKQAEEPKEEDTKLEEDPSAEKPCGADTLAKLQAEQRALAEISKQLRKSVEDLL
FT SADGETVVETQRTYTLPAPSDSSAVVTVTEVVKKQKKKKSETGEELFNRLLNAAESERQ
FT VFDRQQGETITTTTTTTEAVSSEDNEPSTSVVTTTRTVSNIVTSGIPRPETSKQNNRSS
FT SSSNSNSSNNNRNKNKRKGKKK (in isoform K)"
FT /evidence="ECO:0000305"
FT /id="VSP_042867"
FT CONFLICT 19
FT /note="D -> N (in Ref. 2; CAB70601/CAB71137 and 3;
FT AAO32791/AAO32792/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="E -> K (in Ref. 2; CAB70601/CAB71137 and 3;
FT AAO32791/AAO32792/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="G -> D (in Ref. 6; AAT94469)"
FT /evidence="ECO:0000305"
FT CONFLICT 313
FT /note="T -> I (in Ref. 6; AAT94469)"
FT /evidence="ECO:0000305"
FT CONFLICT 1108
FT /note="S -> T (in Ref. 2; CAB70601/CAB71137 and 3;
FT AAO32791/AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1239
FT /note="Missing (in Ref. 3; AAO32791/AAO32792)"
FT /evidence="ECO:0000305"
FT CONFLICT 1326
FT /note="Q -> P (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1340
FT /note="S -> N (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1361
FT /note="N -> Y (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1388
FT /note="K -> Q (in Ref. 1; AAF26357)"
FT /evidence="ECO:0000305"
FT CONFLICT 1540
FT /note="A -> P (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1688
FT /note="D -> Y (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32793/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1700
FT /note="A -> S (in Ref. 7; AAL25508)"
FT /evidence="ECO:0000305"
FT CONFLICT 1741
FT /note="K -> R (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1769
FT /note="I -> F (in Ref. 2; CAB70601 and 3; AAO32791/
FT AAO32792/AAO32794)"
FT /evidence="ECO:0000305"
FT CONFLICT 1833
FT /note="D -> V (in Ref. 6; AAL39806)"
FT /evidence="ECO:0000305"
FT STRAND 726..735
FT /evidence="ECO:0007829|PDB:5WOU"
FT STRAND 742..747
FT /evidence="ECO:0007829|PDB:5WOU"
FT STRAND 760..766
FT /evidence="ECO:0007829|PDB:5WOU"
FT HELIX 771..775
FT /evidence="ECO:0007829|PDB:5WOU"
FT STRAND 782..786
FT /evidence="ECO:0007829|PDB:5WOU"
FT HELIX 796..805
FT /evidence="ECO:0007829|PDB:5WOU"
FT STRAND 808..818
FT /evidence="ECO:0007829|PDB:5WOU"
SQ SEQUENCE 1851 AA; 200440 MW; B744F9BA3827742C CRC64;
MFKCIPIFKG CNRQVEFVDK RHCSLPQVPE EILRYSRTLE ELFLDANHIR DLPKNFFRLH
RLRKLGLSDN EIGRLPPDIQ NFENLVELDV SRNDIPDIPD DIKHLQSLQV ADFSSNPIPK
LPSGFSQLKN LTVLGLNDMS LTTLPADFGS LTQLESLELR ENLLKHLPET ISQLTKLKRL
DLGDNEIEDL PPYLGYLPGL HELWLDHNQL QRLPPELGLL TKLTYLDVSE NRLEELPNEI
SGLVSLTDLD LAQNLLEALP DGIAKLSRLT ILKLDQNRLQ RLNDTLGNCE NMQELILTEN
FLSELPASIG QMTKLNNLNV DRNALEYLPL EIGQCANLGV LSLRDNKLKK LPPELGNCTV
LHVLDVSGNQ LLYLPYSLVN LQLKAVWLSE NQSQPLLTFQ PDTDAETGEQ VLSCYLLPQQ
EYQPITPARD LESDSEPFEE REPSRTVVKF SEEATQEKET PFVRQNTPHP KDLKAKAQKL
KVERSRNEEH ANLVTLPEEN GTKLAETPTE TRTIANNHQQ QPHPVQQPIV GVNSKQPVVV
GVVTPTTTTV APTGVQGGSE GASSTANNVK AATAAVVAEL AATVGGSDEV QDDDEQEDEF
ESDRRVGFQV EGEDDDFYKR PPKLHRRDTP HHLKNKRVQH LTDKQASEIL ANALASQERN
DTTPQHSLSG KVTSPIEEEE QLEVEQEQQQ QQQQHPFDSS LSPISAGKTA EASTDPDNLD
GVTELRLEQY EIHIERTAAG LGLSIAGGKG STPFKGDDDG IFISRVTEAG PADLAGLKVG
DKVIKVNGIV VVDADHYQAV QVLKACGAVL VLVVQREVTR LIGHPVFSED GSVSQISVET
RPLVADAPPA ASISHERYIP APIEIVPQQQ HLQQQQQQPI QQVAPTHSYS GNVFATPTAA
QTVQPAVSAA PNGLLLNGRE APLSYIQLHT TLIRDQIGQG LGFSIAGGKG SPPFKDDCDG
IFISRITEGG LAYRDGKIMV GDRVMAINGN DMTEAHHDAA VACLTEPQRF VRLVLQREYR
GPLEPPTSPR SPAVLNSLSP SGYLANRPAN FSRSVVEVEQ PYKYNTLATT TPTPKPTVPA
SISNNNNTLP SSKTNGFATA AAATIDSSTG QPVPAPRRTN SVPMGDGDIG AGSTTSGDSG
EAQPSSLRPL TSDDFQAMIP AHFLSGGSQH QVHVARPNEV GVSAVTVNVN KPQPDLPMFP
AAPTELGRVT ETITKSTFTE TVMTRITDNQ LAEPLISEEV VLPKNQGSLG FSIIGGTDHS
CVPFGTREPG IFISHIVPGG IASKCGKLRM GDRILKVNEA DVSKATHQDA VLELLKPGDE
IKLTIQHDPL PPGFQEVLLS KAEGERLGMH IKGGLNGQRG NPADPSDEGV FVSKINSVGA
ARRDGRLKVG MRLLEVNGHS LLGASHQDAV NVLRNAGNEI QLVVCKGYDK SNLIHSIGQA
GGMSTGFNSS ASCSGGSRQG SRASETGSEL SQSQSVSSLD HEEDERLRQD FDVFASQKPD
AQQPTGPSVL AAAAAMVHGA SSPTPPAATS NITPLPTAAA VASADLTAPD TPATQTVALI
HAEQQAHQQQ QQTQLAPLGQ EKSTQEKVLE IVRAADAFTT VPPKSPSEHH EQDKIQKTTT
VVISKHTLDT NPTTPTTPAA PLSIAGAESA NSAGAPSPAV PASTPGSAPV LPAVAVQTQT
QTTSTEKDEE EESQLQSTPA SRDGAEEQQE EVRAKPTPTK VPKSVSDKKR FFESAMEDQH
KPTQKTDKVF SFLSKDEVEK LRQEEERKIA TLRRDKNSRL LDAANDNIDK DAAQQRTKSN
SNSSSGDDND DSDQEEGIAR GDSVDNAALG HFDDAEDMRN PLDEIEAVFR S
