ID   SCRK1_MAIZE             Reviewed;         323 AA.
AC   Q6XZ79;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Fructokinase-1;
DE            EC=2.7.1.4;
DE   AltName: Full=ZmFRK1;
GN   Name=FRK1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RA   Zhang S., Nichols S.E., Dong J.G.;
RT   "Cloning and characterization of two fructokinases from maize.";
RL   Plant Sci. 165:1051-1058(2003).
CC   -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC       towards starch formation in endosperm. May also be involved in a sugar-
CC       sensing pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC   -!- ACTIVITY REGULATION: Inhibited at high fructose.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.159 mM for fructose {ECO:0000269|Ref.1};
CC         KM=0.024 mM for ATP {ECO:0000269|Ref.1};
CC         KM=0.491 mM for UTP {ECO:0000269|Ref.1};
CC         Vmax=15400 umol/min/mg enzyme {ECO:0000269|Ref.1};
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- TISSUE SPECIFICITY: Expressed in stems, at higher levels in roots, and
CC       hardly detectable in leaves. {ECO:0000269|Ref.1}.
CC   -!- DEVELOPMENTAL STAGE: Only detected at and after 15 days after
CC       pollination and remains at moderate level. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
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DR   EMBL; AY197772; AAP42805.1; -; mRNA.
DR   RefSeq; NP_001105210.1; NM_001111740.1.
DR   AlphaFoldDB; Q6XZ79; -.
DR   SMR; Q6XZ79; -.
DR   STRING; 4577.GRMZM2G086845_P01; -.
DR   PaxDb; Q6XZ79; -.
DR   PRIDE; Q6XZ79; -.
DR   ProMEX; Q6XZ79; -.
DR   EnsemblPlants; Zm00001eb144910_T001; Zm00001eb144910_P001; Zm00001eb144910.
DR   GeneID; 542107; -.
DR   Gramene; Zm00001eb144910_T001; Zm00001eb144910_P001; Zm00001eb144910.
DR   KEGG; zma:542107; -.
DR   eggNOG; KOG2855; Eukaryota.
DR   OrthoDB; 918144at2759; -.
DR   BRENDA; 2.7.1.4; 6752.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000007305; Chromosome 3.
DR   ExpressionAtlas; Q6XZ79; baseline and differential.
DR   Genevisible; Q6XZ79; ZM.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR   GO; GO:0006000; P:fructose metabolic process; IBA:GO_Central.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR002139; Ribo/fructo_kinase.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   PRINTS; PR00990; RIBOKINASE.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
DR   PROSITE; PS00584; PFKB_KINASES_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..323
FT                   /note="Fructokinase-1"
FT                   /id="PRO_0000234066"
SQ   SEQUENCE   323 AA;  34691 MW;  6D704E78E51B0920 CRC64;
     MAAGRELVVS FGEMLIDFVP TVAGVSLAEA PAFLKAPGGA PANVAIAVSR LGGGAAFVGK
     LGDDEFGRML AAILRDNGVD DGGVVFDSGA RTALAFVTLR ADGEREFMFY RNPSADMLLT
     ADELNVELIK RAAVFHYGSI SLIAEPCRTA HLRAMEIAKE AGALLSYDPN LREALWPSRE
     EARTQILSIW DQADIVKVSE VELEFLTGID SVEDDVVMKL WRPTMKLLLV TLGDQGCKYY
     ARDFHGAVPS FKVQQVDTTG AGDAFVGALL QRIVKDPSSL QDEKKLVESI KFANACGAIT
     TTKKGAIPSL PTEAEVLQLI EKA