ID   SCRK1_ORYSI             Reviewed;         323 AA.
AC   A2WXV8; Q5JLV6; Q7F445; Q944F4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Fructokinase-1;
DE            EC=2.7.1.4;
DE   AltName: Full=Fructokinase I;
DE   AltName: Full=OsFKI;
GN   Name=FRK1; Synonyms=FKI; ORFNames=OsI_004651;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. Zhenshan 773; TISSUE=Immature seed;
RX   PubMed=12475618; DOI=10.1016/s0031-9422(02)00428-4;
RA   Jiang H., Dian W., Liu F., Wu P.;
RT   "Isolation and characterization of two fructokinase cDNA clones from
RT   rice.";
RL   Phytochemistry 62:47-52(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC       towards starch formation. May also be involved in a sugar-sensing
CC       pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC   -!- ACTIVITY REGULATION: Not inhibited at high fructose concentration.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=3.3 mM for fructose {ECO:0000269|PubMed:12475618};
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, endosperms and leaves.
CC       {ECO:0000269|PubMed:12475618}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
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DR   EMBL; AF429948; AAL26574.1; -; mRNA.
DR   EMBL; CM000126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A2WXV8; -.
DR   SMR; A2WXV8; -.
DR   STRING; 39946.A2WXV8; -.
DR   SABIO-RK; A2WXV8; -.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000007015; Chromosome 1.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR002139; Ribo/fructo_kinase.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   PRINTS; PR00990; RIBOKINASE.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
DR   PROSITE; PS00584; PFKB_KINASES_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..323
FT                   /note="Fructokinase-1"
FT                   /id="PRO_0000300857"
FT   CONFLICT        192
FT                   /note="H -> Q (in Ref. 1; AAL26574)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   323 AA;  34729 MW;  6DFF33D544CCEDC4 CRC64;
     MAGRSELVVS FGEMLIDFVP TVAGVSLAEA PAFVKAPGGA PANVAIAVAR LGGGAAFVGK
     LGDDEFGRML AAILRDNGVD DGGVVFDAGA RTALAFVTLR ADGEREFMFY RNPSADMLLT
     HAELNVELIK RAAVFHYGSI SLIAEPCRSA HLRAMEIAKE AGALLSYDPN LREALWPSRE
     EARTKILSIW DHADIVKVSE VELEFLTGID SVEDDVVMKL WRPTMKLLLV TLGDQGCKYY
     ARDFRGAVPS YKVQQVDTTG AGDAFVGALL RRIVQDPSSL QDQKKLEEAI KFANACGAIT
     ATKKGAIPSL PTEVEVLKLM ESA