SCRK1_ORYSJ
ID SCRK1_ORYSJ Reviewed; 323 AA.
AC Q0JGZ6; A0A0P0VBF8; B7E523; Q5JLV6; Q7F445; Q944F4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Fructokinase-1 {ECO:0000305};
DE EC=2.7.1.4 {ECO:0000269|PubMed:12475618};
DE AltName: Full=Fructokinase I {ECO:0000303|PubMed:12475618};
DE AltName: Full=OsFKI {ECO:0000303|PubMed:12475618};
GN Name=FRK1 {ECO:0000305}; Synonyms=FKI {ECO:0000303|PubMed:12475618};
GN OrderedLocusNames=Os01g0894300 {ECO:0000312|EMBL:BAS75687.1},
GN LOC_Os01g66940;
GN ORFNames=B1078G07.45-1 {ECO:0000312|EMBL:BAB90210.1},
GN B1078G07.45-2 {ECO:0000312|EMBL:BAD87551.1},
GN P0696G06.9 {ECO:0000312|EMBL:BAC06252.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ALTERNATIVE
RP SPLICING, ACTIVITY REGULATION, AND TISSUE SPECIFICITY.
RX PubMed=12475618; DOI=10.1016/s0031-9422(02)00428-4;
RA Jiang H., Dian W., Liu F., Wu P.;
RT "Isolation and characterization of two fructokinase cDNA clones from
RT rice.";
RL Phytochemistry 62:47-52(2003).
CC -!- FUNCTION: Fructokinase that may play an important role in maintaining
CC the flux of carbon towards starch formation. May also be involved in a
CC sugar-sensing pathway. {ECO:0000269|PubMed:12475618}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC Evidence={ECO:0000269|PubMed:12475618};
CC -!- ACTIVITY REGULATION: Completely inhibited at 50 mM ATP, but not
CC inhibited at high fructose concentration.
CC {ECO:0000269|PubMed:12475618}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=3.3 mM for fructose {ECO:0000269|PubMed:12475618};
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0JGZ6-1, Q944F4-1;
CC Sequence=Displayed;
CC Name=2;
CC IsoId=Q0JGZ6-2, Q944F4-2;
CC Sequence=VSP_018208;
CC -!- TISSUE SPECIFICITY: Expressed in root, endosperm and leaf tissues.
CC {ECO:0000269|PubMed:12475618}.
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC {ECO:0000305}.
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DR EMBL; AP003316; BAC06252.1; -; Genomic_DNA.
DR EMBL; AP003407; BAB90210.1; -; Genomic_DNA.
DR EMBL; AP003407; BAD87551.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06982.2; -; Genomic_DNA.
DR EMBL; AP014957; BAS75686.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75687.1; -; Genomic_DNA.
DR EMBL; AK060500; BAG87470.1; -; mRNA.
DR EMBL; AK120887; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015621961.1; XM_015766475.1. [Q0JGZ6-1]
DR AlphaFoldDB; Q0JGZ6; -.
DR SMR; Q0JGZ6; -.
DR STRING; 4530.OS01T0894300-01; -.
DR PaxDb; Q0JGZ6; -.
DR PRIDE; Q0JGZ6; -.
DR EnsemblPlants; Os01t0894300-01; Os01t0894300-01; Os01g0894300. [Q0JGZ6-1]
DR EnsemblPlants; Os01t0894300-02; Os01t0894300-02; Os01g0894300. [Q0JGZ6-2]
DR GeneID; 4324962; -.
DR Gramene; Os01t0894300-01; Os01t0894300-01; Os01g0894300. [Q0JGZ6-1]
DR Gramene; Os01t0894300-02; Os01t0894300-02; Os01g0894300. [Q0JGZ6-2]
DR KEGG; osa:4324962; -.
DR eggNOG; KOG2855; Eukaryota.
DR HOGENOM; CLU_027634_6_1_1; -.
DR InParanoid; Q0JGZ6; -.
DR OMA; GPYNTAV; -.
DR OrthoDB; 918144at2759; -.
DR BRENDA; 2.7.1.4; 4460.
DR UniPathway; UPA00152; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q0JGZ6; baseline and differential.
DR Genevisible; Q0JGZ6; OS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008865; F:fructokinase activity; IBA:GO_Central.
DR GO; GO:0006000; P:fructose metabolic process; IBA:GO_Central.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1190.20; -; 1.
DR InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR InterPro; IPR011611; PfkB_dom.
DR InterPro; IPR002139; Ribo/fructo_kinase.
DR InterPro; IPR029056; Ribokinase-like.
DR Pfam; PF00294; PfkB; 1.
DR PRINTS; PR00990; RIBOKINASE.
DR SUPFAM; SSF53613; SSF53613; 1.
DR PROSITE; PS00583; PFKB_KINASES_1; 1.
DR PROSITE; PS00584; PFKB_KINASES_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Carbohydrate metabolism; Kinase;
KW Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..323
FT /note="Fructokinase-1"
FT /id="PRO_0000234064"
FT VAR_SEQ 18..95
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_018208"
SQ SEQUENCE 323 AA; 34720 MW; 6DE00EDD49DBDDC4 CRC64;
MAGRSELVVS FGEMLIDFVP TVAGVSLAEA PAFVKAPGGA PANVAIAVAR LGGGAAFVGK
LGDDEFGRML AAILRDNGVD DGGVVFDAGA RTALAFVTLR ADGEREFMFY RNPSADMLLT
HAELNVELIK RAAVFHYGSI SLIAEPCRSA HLRAMEIAKE AGALLSYDPN LREALWPSRE
EARTKILSIW DQADIVKVSE VELEFLTGID SVEDDVVMKL WRPTMKLLLV TLGDQGCKYY
ARDFRGAVPS YKVQQVDTTG AGDAFVGALL RRIVQDPSSL QDQKKLEEAI KFANACGAIT
ATKKGAIPSL PTEVEVLKLM ESA
