ID   SCRK2_MAIZE             Reviewed;         335 AA.
AC   Q6XZ78;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Fructokinase-2;
DE            EC=2.7.1.4;
DE   AltName: Full=ZmFRK2;
GN   Name=FRK2;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RA   Zhang S., Nichols S.E., Dong J.G.;
RT   "Cloning and characterization of two fructokinases from maize.";
RL   Plant Sci. 165:1051-1058(2003).
CC   -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC       towards starch formation. May also be involved in a sugar-sensing
CC       pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC   -!- ACTIVITY REGULATION: Inhibited at high fructose.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.109 mM for fructose {ECO:0000269|Ref.1};
CC         KM=0.187 mM for ATP {ECO:0000269|Ref.1};
CC         KM=0.222 mM for UTP {ECO:0000269|Ref.1};
CC         Vmax=835 umol/min/mg enzyme {ECO:0000269|Ref.1};
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, at higher levels in stems, and
CC       hardly detectable in leaves. {ECO:0000269|Ref.1}.
CC   -!- DEVELOPMENTAL STAGE: High expression 7 days after pollination (DAP),
CC       gradually declines after 15 DAP and becomes undetectable by 29 DAP.
CC       {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
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DR   EMBL; AY197773; AAP42806.1; -; mRNA.
DR   RefSeq; NP_001105211.1; NM_001111741.2.
DR   AlphaFoldDB; Q6XZ78; -.
DR   SMR; Q6XZ78; -.
DR   STRING; 4577.GRMZM2G051677_P02; -.
DR   PaxDb; Q6XZ78; -.
DR   PRIDE; Q6XZ78; -.
DR   EnsemblPlants; Zm00001eb260710_T001; Zm00001eb260710_P001; Zm00001eb260710.
DR   GeneID; 542108; -.
DR   Gramene; Zm00001eb260710_T001; Zm00001eb260710_P001; Zm00001eb260710.
DR   KEGG; zma:542108; -.
DR   eggNOG; KOG2855; Eukaryota.
DR   HOGENOM; CLU_027634_6_1_1; -.
DR   OMA; AIVKQGP; -.
DR   OrthoDB; 918144at2759; -.
DR   BRENDA; 2.7.1.4; 6752.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000007305; Chromosome 6.
DR   ExpressionAtlas; Q6XZ78; baseline and differential.
DR   Genevisible; Q6XZ78; ZM.
DR   GO; GO:0005829; C:cytosol; IDA:AgBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IDA:AgBase.
DR   GO; GO:0046835; P:carbohydrate phosphorylation; IDA:AgBase.
DR   GO; GO:0006000; P:fructose metabolic process; IBA:GO_Central.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..335
FT                   /note="Fructokinase-2"
FT                   /id="PRO_0000234067"
SQ   SEQUENCE   335 AA;  35482 MW;  346F455D2B3D1C5A CRC64;
     MAPLGDGGAA AAAASNNLVV SFGEMLIDFV PDVAGLSLAE SGGFVKAPGG APANVACAIA
     KLGGSSAFVG KFGDDEFGHM LVNILKQNNV NAEGCLFDKH ARTALAFVTL KHDGEREFMF
     YRNPSADMLL TEAELDLGLV RRARVFHYGS ISLISEPCRS AHMAAMRAAK AAGVLCSYDP
     NVRLPLWPSP DAAREGILSI WKEADFIKVS DDEVAFLTRG DANDEKNVLS LWFDGLKLLV
     VTDGDKGCRY FTKDFKGSVP GFKVDTVDTT GAGDAFVGSL LVNVAKDDSI FHNEEKLREA
     LKFSNACGAI CTTKKGAIPA LPTVATAQDL IAKAN