ID   SCRK2_ORYSI             Reviewed;         336 AA.
AC   A2YQL4; Q7XXS9; Q944F5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Fructokinase-2;
DE            EC=2.7.1.4;
DE   AltName: Full=Fructokinase II;
DE   AltName: Full=OsFKII;
GN   Name=FRK2; Synonyms=FKII; ORFNames=OsI_026607;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Zhenshan 773; TISSUE=Immature seed;
RX   PubMed=12475618; DOI=10.1016/s0031-9422(02)00428-4;
RA   Jiang H., Dian W., Liu F., Wu P.;
RT   "Isolation and characterization of two fructokinase cDNA clones from
RT   rice.";
RL   Phytochemistry 62:47-52(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: May play an important role in maintaining the flux of carbon
CC       towards starch formation in endosperm. May also be involved in a sugar-
CC       sensing pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC         Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.4;
CC   -!- ACTIVITY REGULATION: Strongly inhibited at high fructose concentration.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.34 mM for fructose {ECO:0000269|PubMed:12475618};
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- TISSUE SPECIFICITY: Predominantly in roots and endosperms and low
CC       levels in mature leaves. {ECO:0000269|PubMed:12475618}.
CC   -!- DEVELOPMENTAL STAGE: Expressed 3 days after flowering, decreases at 8
CC       days after flowering and then remains constant.
CC       {ECO:0000269|PubMed:12475618}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
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DR   EMBL; AF429947; AAL26573.1; -; mRNA.
DR   EMBL; CM000133; EAZ05375.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2YQL4; -.
DR   SMR; A2YQL4; -.
DR   STRING; 39946.A2YQL4; -.
DR   HOGENOM; CLU_027634_6_1_1; -.
DR   BRENDA; 2.7.1.4; 4460.
DR   SABIO-RK; A2YQL4; -.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000007015; Chromosome 8.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008865; F:fructokinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Carbohydrate metabolism; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..336
FT                   /note="Fructokinase-2"
FT                   /id="PRO_0000300858"
FT   CONFLICT        14
FT                   /note="Missing (in Ref. 2; EAZ05375)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   336 AA;  35517 MW;  FFC33297A2AC1A92 CRC64;
     MAPLGDGAAA AAAAEPNLVV SFGEMLIDFV PDVAGVSLAE SGGFVKAPGG APANVACAIS
     KLGGSSAFVG KFGDDEFGHM LVDILKKNGV NAEGCLFDEH ARTALAFVTL KSNGEREFMF
     YRNPSADMLL TEAELNLDLI RRAKIFHYGS ISLITEPCRS AHVAAMRAAK SAGILCSYDP
     NVRLPLWPSE DAARAGILSI WKEADFIKVS DDEVAFLTQG DANDEKNVLS LWFDGLKLLI
     VTDGEKGCRY FTKDFKGSVP GFSVNTVDTT GAGDAFVGSL LVNVAKDDSI FHNEEKLREA
     LKFSNACGAI CTTKKGAIPA LPTVAVAQEL ISKAAN